Transcriptional response of bovine monocyte - derived macrophages after the infection with different argentinean mycobacterium bovis isolates

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Infection of bovines with Mycobacterium bovis causes important financial hardship in many countries presenting also a risk for humans. M. bovis is known to be adapted to survive and thrive within the intramacrophage environment. In spite of its relevance, at present the information about macrophage...

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Detalles Bibliográficos
Otros Autores: Caimi, Karina, Blanco, Federico Carlos, Soria, Marcelo Abel, Bigi, Fabiana
Formato: Artículo
Lenguaje:Inglés
Materias:
ANKYRIN
ANTHRAX TOXIN
CYTOSKELETON PROTEIN
TRANSCRIPTION FACTOR
APOPTOSIS
BACTERIAL STRAIN
BACTERIUM ISOLATE
BOVINE TUBERCULOSIS
CELL DIFFERENTIATION
CELL DIVISION
GENE EXPRESSION
MICROARRAY ANALYSIS
MYCOBACTERIUM BOVIS
NONHUMAN
PERIPHERAL BLOOD MONONUCLEAR CELL
REAL TIME POLYMERASE CHAIN REACTION
TRANSCRIPTOMICS
CATTLE
GENE EXPRESSION REGULATION
GENETIC TRANSCRIPTION
IMMUNOLOGY
ISOLATION AND PURIFICATION
METABOLISM
MICROBIOLOGY
ANIMALS
ARGENTINA
MACROPHAGES
MONOCYTES
TRANSCRIPTION, GENETIC
TUBERCULOSIS, BOVINE
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2013caimi.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Transcriptional response of bovine monocyte - derived macrophages after the infection with different argentinean mycobacterium bovis isolates 
520 |a Infection of bovines with Mycobacterium bovis causes important financial hardship in many countries presenting also a risk for humans. M. bovis is known to be adapted to survive and thrive within the intramacrophage environment. In spite of its relevance, at present the information about macrophage expression patterns is scarce, particularly regarding the bovine host. In this study, transcriptomic analysis was used to detect genes differentially expressed in macrophages derived from peripheral blood mononuclear cells at early stages of infection with two Argentinean strains of M. bovis, a virulent and an attenuated strains. The results showed that the number of differentially expressed genes in the cells infected with the virulent strain [5] was significantly lower than those in the cells infected with the attenuated strain [172]. Several genes were more strongly expressed in infected macrophages. Among them, we detected encoding transcription factors, anthrax toxin receptor, cell division and apoptosis regulator, ankyrin proteins, cytoskeleton proteins, protein of cell differentiation, and regulators of endocytic traffic of membrane. Quantitative real-time PCR of a selected group of differentially expressed genes confirmed the microarrays results. Altogether, the present results contribute to understanding the mechanisms involved in the early interaction of M. bovis with the bovine macrophage. 
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773 |t BioMed Research International  |g vol.2013 (2013), p.1-9 
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900 |a ^aCaimi, K.^tBiotechnology Institute, INTA Castelar, N. Repetto y Los Reseros s/n (1686), 1712 Hurlingham, Buenos Aires, Argentina 
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900 |a ^aSoria, M.^tCollege of Agronomy, University of Buenos Aires, INBA-CONICET, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina 
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900 |a CELL DIFFERENTIATION 
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900 |a TRANSCRIPTOMICS 
900 |a CATTLE 
900 |a GENE EXPRESSION REGULATION 
900 |a GENETIC TRANSCRIPTION 
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900 |a ISOLATION AND PURIFICATION 
900 |a METABOLISM 
900 |a MICROBIOLOGY 
900 |a ANIMALS 
900 |a ARGENTINA 
900 |a MACROPHAGES 
900 |a MONOCYTES 
900 |a TRANSCRIPTION, GENETIC 
900 |a TUBERCULOSIS, BOVINE 
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