In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2 - OXIDASE promoter possible role of this interaction in the expression of seed dormancy
The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum [Sorghum bicolor [L.] Moench] shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting [PHS]. Both embryo sensit...
| Otros Autores: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/download/articulo/2013cantoro.pdf LINK AL EDITOR |
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| 245 | 1 | 0 | |a In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2 - OXIDASE promoter |b possible role of this interaction in the expression of seed dormancy |
| 520 | |a The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum [Sorghum bicolor [L.] Moench] shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting [PHS]. Both embryo sensitivity to abscisic acid [ABA] and gibberellin [GA] metabolism play an important role in the expression of dormancy of the developing sorghum grain. In previous works, it was shown that, simultaneously with a greater embryo sensitivity to ABA and higher expression of SbABA-INSENSITIVE 4 [SbABI4] and SbABA-INSENSITIVE 5 [SbABI5], dormant grains accumulate less active GA4 due to a more active GA catabolism. In this work, it is demonstrated that the ABA signalling components SbABI4 and SbABI5 interact in vitro with a fragment of the SbGA 2-OXIDASE 3 [SbGA2ox3] promoter containing an ABA-responsive complex [ABRC]. Both transcription factors were able to bind the promoter, although not simultaneously, suggesting that they might compete for the same cis-acting regulatory sequences. A biological role for these interactions in the expression of dormancy of sorghum grains is proposed: either SbABI4 and/or SbABI5 activate transcription of the SbGA2ox3 gene in vivo and promote SbGA2ox3 protein accumulation; this would result in active degradation of GA4, thus preventing germination of dormant grains. A comparative analysis of the 5'-regulatory region of GA2oxs from both monocots and dicots is also presented; conservation of the ABRC in closely related GA2oxs from Brachypodium distachyon and rice suggest that these species might share the same regulatory mechanism as proposed for grain sorghum. | ||
| 653 | 0 | |a ABI4 | |
| 653 | 0 | |a ABI5 | |
| 653 | 0 | |a ABSCISIC ACID | |
| 653 | 0 | |a GA 2-OXIDASE | |
| 653 | 0 | |a GERMINATION | |
| 653 | 0 | |a GIBBERELLINS | |
| 653 | 0 | |a SEED DORMANCY | |
| 653 | 0 | |a SORGHUM BICOLOR | |
| 700 | 1 | |9 36043 |a Cantoro, Renata | |
| 700 | 1 | |9 37590 |a Crocco, Carlos Daniel | |
| 700 | 1 | |9 663 |a Benech Arnold, Roberto Luis | |
| 700 | 1 | |9 58187 |a Rodríguez, María Verónica | |
| 773 | |t Journal of Experimental Botany |g vol.64, no.18 (2013), p.5721-5735 | ||
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| 900 | |a ^tIn vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter^sPossible role of this interaction in the expression of seed dormancy | ||
| 900 | |a ^aCantoro^bR. | ||
| 900 | |a ^aCrocco^bC.D. | ||
| 900 | |a ^aBenech-Arnold^bR.L. | ||
| 900 | |a ^aRodríguez^bM.V. | ||
| 900 | |a ^aCantoro^bR. | ||
| 900 | |a ^aCrocco^bC. D. | ||
| 900 | |a ^aBenech Arnold^bR. L. | ||
| 900 | |a ^aRodríguez^bM. V. | ||
| 900 | |a ^aCantoro^bR.^tIFEVA, Facultad de AgronomÃa, Universidad de Buenos Aires, CONICET, Av. San MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aCrocco^bC.D.^tCátedra de Cultivos Industriales, Facultad de AgronomÃa, Universidad de Buenos Aires, Av. San MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aBenech-Arnold^bR.L. | ||
| 900 | |a ^aRodrÃguez^bM.V. | ||
| 900 | |a ^aCantoro, R^tIFEVA, Facultad de AgronomÃa, Universidad de Buenos Aires, CONICET, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aCantoro, R^tCátedra de Cultivos Industriales, Facultad de AgronomÃa, Universidad de Buenos Aires, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aCrocco, C.D^tIFEVA, Facultad de AgronomÃa, Universidad de Buenos Aires, CONICET, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aBenech-Arnold, R.L^tIFEVA, Facultad de AgronomÃa, Universidad de Buenos Aires, CONICET, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aBenech-Arnold, R.L^tCátedra de Cultivos Industriales, Facultad de AgronomÃa, Universidad de Buenos Aires, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^aRodrÃguez, M.V^tIFEVA, Facultad de AgronomÃa, Universidad de Buenos Aires, CONICET, Av^tSan MartÃn 4453, C1417DSE Caba, Argentina | ||
| 900 | |a ^tJournal of Experimental Botany^cJ. Exp. Bot. | ||
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| 900 | |a Vol. 64, no. 18 | ||
| 900 | |a 5735 | ||
| 900 | |a ABI4 | ||
| 900 | |a ABI5 | ||
| 900 | |a ABSCISIC ACID | ||
| 900 | |a GA 2-OXIDASE | ||
| 900 | |a GERMINATION | ||
| 900 | |a GIBBERELLINS | ||
| 900 | |a SEED DORMANCY | ||
| 900 | |a SORGHUM BICOLOR | ||
| 900 | |a The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum [Sorghum bicolor [L.] Moench] shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting [PHS]. Both embryo sensitivity to abscisic acid [ABA] and gibberellin [GA] metabolism play an important role in the expression of dormancy of the developing sorghum grain. In previous works, it was shown that, simultaneously with a greater embryo sensitivity to ABA and higher expression of SbABA-INSENSITIVE 4 [SbABI4] and SbABA-INSENSITIVE 5 [SbABI5], dormant grains accumulate less active GA4 due to a more active GA catabolism. In this work, it is demonstrated that the ABA signalling components SbABI4 and SbABI5 interact in vitro with a fragment of the SbGA 2-OXIDASE 3 [SbGA2ox3] promoter containing an ABA-responsive complex [ABRC]. Both transcription factors were able to bind the promoter, although not simultaneously, suggesting that they might compete for the same cis-acting regulatory sequences. A biological role for these interactions in the expression of dormancy of sorghum grains is proposed: either SbABI4 and/or SbABI5 activate transcription of the SbGA2ox3 gene in vivo and promote SbGA2ox3 protein accumulation; this would result in active degradation of GA4, thus preventing germination of dormant grains. A comparative analysis of the 5'-regulatory region of GA2oxs from both monocots and dicots is also presented; conservation of the ABRC in closely related GA2oxs from Brachypodium distachyon and rice suggest that these species might share the same regulatory mechanism as proposed for grain sorghum. | ||
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