Optimization of donor cell cycle synchrony, maturation media and embryo culture system for somatic cell nuclear transfer in the critically endangered Vietnamese Ỉ pig
Our aim was to establish an efficient culture system to produce embryos by SCNT of the endangered Vietnamese Ỉ pig. Reducing the serum concentration from 10.0% to 0.2% during culture efficiently synchronized Ỉ pig fibroblasts used as donor cells at the G0/G1 stage. Oocyte maturation in a defined por...
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| Otros Autores: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Materias: | |
| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2021nguyen.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
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| 022 | |a 0093-691X | ||
| 024 | |a 10.1016/j.theriogenology.2021.02.008 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | |a Optimization of donor cell cycle synchrony, maturation media and embryo culture system for somatic cell nuclear transfer in the critically endangered Vietnamese Ỉ pig | |
| 520 | |a Our aim was to establish an efficient culture system to produce embryos by SCNT of the endangered Vietnamese Ỉ pig. Reducing the serum concentration from 10.0% to 0.2% during culture efficiently synchronized Ỉ pig fibroblasts used as donor cells at the G0/G1 stage. Oocyte maturation in a defined porcine oocyte medium (POM) supplemented with EGF and gonadotrophins resulted in higher cleavage and blastocyst rates compared with a non-defined POM containing pig follicular fluid (but without EGF) and both the defined and non-defined variants of NCSU-37. For embryo culture PZM3 and PZM5 media were superior to NCSU-37, in terms of the percentage of cleaved embryos. Addition of serum to PZM3 medium on Day 5 of culture (Day 0 ¼ SCNT) improved blastocyst development. When SCNT embryos were transferred at the blastocyst stage, 7 of 11 recipients became pregnant. However, live offspring were not obtained. In conclusion, we established a system for the production of Ỉ pig embryos by SCNT and achieved blastocyst production rate at 26.4% by improving culture systems for donor cells, oocytes and embryos culture. Transfer of embryos resulted in pregnancies; however, live offspring were not obtained. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | |a VIETNAMESE I PIG | ||
| 653 | |a SOMATIC CELL NUCLEAR TRANSFER | ||
| 653 | |a CULTURE SYSTEM | ||
| 653 | |a EMBRYO PRODUCTION | ||
| 653 | |a EMBRYO TRANSFER | ||
| 700 | 1 | |a Nguyen, Van Khanh |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73773 | |
| 700 | 1 | |a Somfai, Tamas |u Institute of Livestock and Grassland Science. Animal Breeding and Reproduction Research Division. NARO, Tsukuba, Japan. |9 73774 | |
| 700 | 1 | |a Salamone, Daniel Felipe |u Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina. |u CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina. |9 61021 | |
| 700 | 1 | |a Thu Huong, Vu Thi |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73775 | |
| 700 | 1 | |a Nguyen, Huong Le Thi |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73776 | |
| 700 | 1 | |a Huu, Quan Xuan |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73777 | |
| 700 | 1 | |a Hoang, Au Thi |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73778 | |
| 700 | 1 | |a Phan, Hieu Trung |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73779 | |
| 700 | 1 | |a Pham, Yen Kim Thi |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73868 | |
| 700 | 1 | |a Pham, Lan Doan |u National Institute of Animal Science. Key Lab of Animal Cell Biotechnology. Hanoi, Viet Nam. |9 73869 | |
| 773 | |t Theriogenology |g Vol.166 (2021), p.21-28, tbls., fot. | ||
| 856 | |f 2021nguyen |i en reservorio |q application/pdf |u http://ri.agro.uba.ar/files/intranet/articulo/2021nguyen.pdf |x ARTI202206 | ||
| 856 | |u http://www.elsevier.com/ |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||