Localization and survival of Azospirillum brasilense Az39 in soybean leaves
In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial microorganisms to crops under field conditions. Colonization efficiency by such microorganisms largely depends on their ability to survive when applied on the leaves. In this work, we ev...
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| Formato: | Artículo |
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2021puente.pdf LINK AL EDITOR |
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| 245 | 1 | |a Localization and survival of Azospirillum brasilense Az39 in soybean leaves | |
| 520 | |a In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial microorganisms to crops under field conditions. Colonization efficiency by such microorganisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron-m icroscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain. | ||
| 653 | |a AZOSPIRILLUM BRASILENSE | ||
| 653 | |a FOLIAR INOCULACION | ||
| 653 | |a LEAF LOCALIZATION | ||
| 653 | |a PGPR | ||
| 653 | |a SOYBEAN | ||
| 700 | 1 | |a Puente, Mariana Laura |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (INTA-IMYZA). Castelar, Argentina. |9 50357 | |
| 700 | 1 | |a Maronichea, Guillermo A. |u Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Balcarce, Buenos Aires, Argentina. |u CONICET - Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Balcarce, Buenos Aires, Argentina. |9 50358 | |
| 700 | 1 | |a Panepucci, M. |u Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina. |9 73625 | |
| 700 | 1 | |a Sabio y Garcıa, J. |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina. |u CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina. |9 73626 | |
| 700 | 1 | |a García, J. E. |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (INTA-IMYZA). Castelar, Argentina. |9 46014 | |
| 700 | 1 | |a Criado, María Victoria |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. |9 36121 | |
| 700 | |a Molina, R. |u Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta - Microorganismo (INIAB) (CONICET). Río Cuarto, Argentina. |9 46343 | ||
| 700 | 1 | |a Cassán, Fabricio Darío |u Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta - Microorganismo (INIAB) (CONICET). Río Cuarto, Argentina. |9 50353 | |
| 773 | |t Letters in Applied Microbiology |g Vol.72, no.5 (2020), p.626-633, tbls., fot. | ||
| 856 | |f 2021puente |i en reservorio |q application/pdf |u http://ri.agro.uba.ar/files/intranet/articulo/2021puente.pdf |x ARTI202206 | ||
| 856 | |u http://www.wiley.com/ |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||