Genomic insights into the potent antifungal activity of B. ambifaria T16

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Burkholderia ambifaria T16 is a bacterial strain isolated from the rhizosphere of barley plants, which showed a potent antifungal activity against several Fusarium species. In this work, the construction of a mini-Tn5 insertional library in this strain allowed us the identification of several genes...

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Detalles Bibliográficos
Otros Autores: Simonetti, Ester, Alvarez, Florencia, Feldman, Nicolás, Vinacour, Matías, Roberts, Irma N., Ruiz, Jimena A.
Formato: Artículo
Lenguaje:Inglés
Materias:
BURKHOLDERIA AMBIFARIA
LIPOPEPTIDES
BIOSYNTHESIS CLUSTERS
OCCIDIOFUNGINS
FUSARIUM
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2021simonetti.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 |a Genomic insights into the potent antifungal activity of B. ambifaria T16 
520 |a Burkholderia ambifaria T16 is a bacterial strain isolated from the rhizosphere of barley plants, which showed a potent antifungal activity against several Fusarium species. In this work, the construction of a mini-Tn5 insertional library in this strain allowed us the identification of several genes involved in the antifungal activity. In particular, several mutants with mini-Tn5 insertions located in a biosynthetic gene cluster (BGC) involved in the production of cyclic glycolipopeptides known as occidiofungins (Ocfs) were completely unable to inhibit the in vitro growth of F. oxysporum. Tandem mass spectrometry analysis of lipopeptide extracts obtained from culture supernatants of the wild-type strain allowed the identification of eight Ocfs structural variants, that were not detected in extracts of the mutants with insertions in the ocf gene cluster. These results indicate that the antifungal activity of B. ambifaria T16 mainly relies on Ocfs production. Moreover, an insertion in a BGC predicted to be involved in the biosynthesis of a membrane-associated lipopeptide designated AFC-BC11 reduced the antifungal activity of B. ambifaria T16 by approximately 50%. Accordingly, membrane extracts from the insertional mutant in the afc-BC11 cluster showed a marked reduction in the antifungal activity against several fungi. In contrast, the antifungal activity of the membrane fraction of mutants unable to produce Ocfs was not affected. Furthermore, a detailed bioinformatic analysis of the proteins encoded by the ocf and afc-BC11 gene clusters revealed that the biosynthesis of these lipopeptides would be initiated by fatty acyl-AMP ligase (FAAL) domain containing enzymes and continued by a hybrid non-ribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) or a type III PKS, respectively. These results provide new insights into lipopeptide biosynthesis, which may be valuable in the field of novel antifungal compound design. 
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653 |a BURKHOLDERIA AMBIFARIA 
653 |a LIPOPEPTIDES 
653 |a BIOSYNTHESIS CLUSTERS 
653 |a OCCIDIOFUNGINS 
653 |a FUSARIUM 
700 1 |a Simonetti, Ester  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |9 50328 
700 1 |a Alvarez, Florencia  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |9 69756 
700 1 |a Feldman, Nicolás  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |9 73570 
700 1 |a Vinacour, Matías  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |9 73571 
700 1 |a Roberts, Irma N.  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |9 46844 
700 1 |9 72778  |a Ruiz, Jimena A.  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. 
773 0 |t Biological control  |w SECS000498  |g Vol.155 (2021), art.104530, 12 p., il., grafs., tbls., fot. 
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976 |a AAG 

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