Effect of phytase supplementation of a plant - based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata

To assess to what extent addition of phytase to a plant based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stoma...

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Otros Autores: Morales, Gabriel A., Márquez, Lorenzo, Saenz de Rodrigañez, M., Bermúdez, L., Robles, R., Moyano, Francisco J.
Formato: Artículo
Lenguaje:Inglés
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Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2014morales.pdf
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245 1 |a Effect of phytase supplementation of a plant - based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata 
520 |a To assess to what extent addition of phytase to a plant based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P minor to 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH mayor to 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P minor 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture. 
653 |a AMINO ACIDS 
653 |a DIGESTIVE PROTEASES 
653 |a PHOSPHORUS 
653 |a PHYTASE 
653 |a PHYTATE 
653 |a SPARUS AURATA 
700 1 |a Morales, Gabriel A.  |u Universidad de Almería. Escuela Politécnica Superior. Departamento de biología aplicada. Almería, España.  |9 14613 
700 1 |a Márquez, Lorenzo  |u Universidad de Almería. Escuela Politécnica Superior. Departamento de biología aplicada. Almería, España.  |9 67731 
700 1 |a Saenz de Rodrigañez, M.  |u Universidad de Almería. Escuela Politécnica Superior. Departamento de biología aplicada. Almería, España.  |9 69526 
700 1 |a Bermúdez, L.  |u Fundación Centro Tecnológico de Acuicultura de Andalucía. Cádiz, España.  |9 69527 
700 1 |a Robles, R.  |u Fundación Centro Tecnológico de Acuicultura de Andalucía. Cádiz, España.  |9 69528 
700 1 |a Moyano, Francisco J.  |u Universidad de Almería. Escuela Politécnica Superior. Departamento de biología aplicada. Almería, España.  |9 61024 
773 |g vol.20, no.2 (2014), p.172-182, grafs.  |t Aquaculture nutrition 
856 |f 2014morales  |i En Reservorio:  |q application/pdf  |u http://ri.agro.uba.ar/files/intranet/articulo/2014morales.pdf  |x ARTI201911 
856 |u https://www.wiley.com  |z LINK AL EDITOR 
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