Establishment of cell - based transposon - mediated transgenesis in cattle
Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac [PB] and sleeping beauty [SB] transposon systems were assessed for st...
| Otros Autores: | , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Materias: | |
| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2016alessio.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
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| 008 | 181208t2016 |||||o|||||00||||eng d | ||
| 999 | |c 47363 |d 47363 | ||
| 999 | |d 47363 | ||
| 022 | |a 0093-691X | ||
| 024 | |a 10.1016/j.theriogenology.2015.12.016 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | 0 | |a Establishment of cell - based transposon - mediated transgenesis in cattle |
| 520 | |a Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac [PB] and sleeping beauty [SB] transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes [fluorophore and neomycin resistance]. Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transposition is a feasible approach for genetic engineering in the cattle genome. | ||
| 653 | 0 | |a CATTLE | |
| 653 | 0 | |a PIGGY BAC | |
| 653 | 0 | |a SLEEPING BEAUTY | |
| 653 | 0 | |a TRANSGENESIS | |
| 653 | 0 | |a TRANSPOSON | |
| 700 | 1 | |a Alessio, A. P. |9 70155 | |
| 700 | 1 | |a Fili, Alejandro E. |9 70154 | |
| 700 | 1 | |a Garrels, Wiebke |9 70156 | |
| 700 | 1 | |a Forcato, Diego O. |9 70157 | |
| 700 | 1 | |a Olmos Nicotra, María F. |9 70158 | |
| 700 | 1 | |a Liaudat, Ana C. |9 70159 | |
| 700 | 1 | |a Bevacqua, Romina Jimena |9 67357 | |
| 700 | 1 | |a Savy, Virginia |9 68562 | |
| 700 | 1 | |9 37270 |a Hiriart, María Inés | |
| 700 | 1 | |a Talluri, Thirumala R. |9 70160 | |
| 700 | 1 | |a Owens, Jesse B. |9 70161 | |
| 700 | 1 | |a Ivics, Zoltán |9 70162 | |
| 700 | 1 | |9 61021 |a Salamone, Daniel Felipe | |
| 700 | 1 | |a Moisyadi, Stefan |9 70163 | |
| 700 | 1 | |a Kues, Wilfried A. |9 70164 | |
| 700 | 1 | |a Bosch, Pablo |9 70165 | |
| 773 | |t Theriogenology |g vol.85, no.7 (2016), p.1297-1311, tbls., grafs., fot. | ||
| 856 | |u http://ri.agro.uba.ar/files/intranet/articulo/2016alessio.pdf |i En reservorio |q application/pdf |f 2016alessio |x MIGRADOS2018 | ||
| 856 | |u https://www.elsevier.com |x MIGRADOS2018 |z LINK AL EDITOR | ||
| 942 | 0 | 0 | |c ARTICULO |
| 942 | 0 | 0 | |c ENLINEA |
| 976 | |a AAG | ||