A Japanese plum Alpha - L- arabinofuranosidase / Beta - D- xylosidase gene is developmentally regulated by alternative splicing
A full-length cDNA clone named PsARF/XYL was obtained from Prunus salicina Lindl., and determined to encode a putative Alpha-L-arabinofuranosidase/Beta-D-xylosidase belonging to glycoside hydrolase [GH, EC 3.2.1.-] family 3. Two related PsARF/XYL cDNAs were amplified, one from a fully-spliced transc...
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| Otros Autores: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Español |
| Materias: | |
| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2015disanto.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
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| 245 | 1 | 0 | |a A Japanese plum Alpha - L- arabinofuranosidase / Beta - D- xylosidase gene is developmentally regulated by alternative splicing |
| 520 | |a A full-length cDNA clone named PsARF/XYL was obtained from Prunus salicina Lindl., and determined to encode a putative Alpha-L-arabinofuranosidase/Beta-D-xylosidase belonging to glycoside hydrolase [GH, EC 3.2.1.-] family 3. Two related PsARF/XYL cDNAs were amplified, one from a fully-spliced transcript [PsARF/XYLa] and another one from an intron-retained transcript [PsARF/XYLb]. The protein deduced from PsARF/XYLb is a truncated peptide at C-terminus that conserves the active-site amino acid sequence. High levels of PsARF/XYLa and PsARF/XYLb transcripts are detectable in several plant tissues. PsARF/XYLb transcripts accumulate progressively during the phase of exponential fruit growth but they become barely noticeable during on-tree ripening, or after a 6-h exposure of preclimacteric full-size plums to ethylene. In contrast, PsARF/XYLa is expressed throughout fruit development, and transcript accumulation parallels the climacteric rise in ethylene production during ripening. PsARF/XYLa expression is strongly induced in preclimacteric full-size plums after a 6-h treatment with physiologically active concentrations of ethylene. These findings suggest that PsARF/XYL gene is post-transcriptionally regulated by alternative splicing during development and that ethylene may be involved in this regulation. The isolation of a partial cDNA clone, PsARF1, is also reported. It encodes a putative cell-wall Alpha-L-arabinofuranosidase, and its transcription is rapidly inhibited by ethylene in mature green plums. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | 0 | |a SPLICING EVENT | |
| 653 | 0 | |a RIPENING | |
| 653 | 0 | |a PRUNUS SALICINA | |
| 653 | 0 | |a INTRON RETENTION | |
| 653 | 0 | |a ETHYLENE | |
| 700 | 1 | |a Di Santo, María Carolina |9 71398 | |
| 700 | 1 | |9 34110 |a Ilina, Natalia | |
| 700 | 1 | |9 6303 |a Pagano, Eduardo Antonio | |
| 700 | 1 | |9 10078 |a Sozzi, Gabriel Oscar | |
| 773 | |t Plant Science |g vol.231 (2015), p.173-183 | ||
| 856 | |u http://ri.agro.uba.ar/files/intranet/articulo/2015disanto.pdf |i En reservorio |q application/pdf |f 2015disanto |x MIGRADOS2018 | ||
| 856 | |u https://www.elsevier.com/ |x MIGRADOS2018 |z LINK AL EDITOR | ||
| 942 | 0 | 0 | |c ARTICULO |
| 942 | 0 | 0 | |c ENLINEA |
| 976 | |a AAG | ||