Assessment of the immune responses induced in cattle after inoculation of a Mycobacterium bovis strain deleted in two mce2 genes

Mostrar otras versiones (1)

The generation of efficient candidate vaccines against bovine tuberculosis will contribute to the control of this zoonotic disease. Rationally attenuated Mycobacterium bovis strains generated by knockout of virulence genes are promising candidate vaccines. However, to be effective, these candidate v...

Descripción completa

Guardado en:
Detalles Bibliográficos
Otros Autores: Blanco, Federico Carlos, Soria, Marcelo Abel, Gravisaco, María José, Bianco, María Verónica, Meikle, Virginia, Garbaccio, Sergio, Vagnoni, Lucas, Cataldi, Angel Adrián, Bigi, Fabiana
Formato: Artículo
Lenguaje:Español
Materias:
ANIMAL
ANIMALIA
ANIMALS
ANTIGENS, BACTERIAL
BACTERIAL ANTIGEN
BACTERIAL PROTEIN
BACTERIAL PROTEINS
BACTERIAL STRAIN
BCG VACCINE
BOS
BOVINAE
BOVINE TUBERCULOSIS
CATTLE
CD4+ T LYMPHOCYTE
CD4-POSITIVE T-LYMPHOCYTES
CD8+ T LYMPHOCYTE
CD8-POSITIVE T-LYMPHOCYTES
CELL CULTURE
CELLS, CULTURED
CYTOKINE
CYTOKINES
GENE
GENE DELETION
GENE INACTIVATION
GENE KNOCKOUT TECHNIQUES
GENE MUTATION
GENETICS
HOST PATHOGEN INTERACTION
HOST-PATHOGEN INTERACTIONS
IMMUNE RESPONSE
IMMUNOLOGY
IN VITRO STUDY
INOCULATION
LEUKOCYTES, MONONUCLEAR
LYMPHOCYTE ACTIVATION
MCE2 GENE
MESSENGER RNA
METABOLISM
MONONUCLEAR CELL
MYCOBACTERIUM BOVIS
PERIPHERAL BLOOD MONONUCLEAR CELL
POLYMERASE CHAIN REACTION
RNA, MESSENGER
TH1 CELL
TUBERCULOSIS VACCINES
TUBERCULOSIS, BOVINE
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012blanco1.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
LEADER 03744cab a22008897a 4500
001 AR-BaUFA000748
003 AR-BaUFA
005 20230405123902.0
008 181208t2012 |||||o|||||00||||spa d
999 |c 47144  |d 47144 
999 |d 47144 
022 |a 1110-7243 
024 |a 10.1155/2012/258353 
040 |a AR-BaUFA 
245 1 0 |a Assessment of the immune responses induced in cattle after inoculation of a Mycobacterium bovis strain deleted in two mce2 genes 
520 |a The generation of efficient candidate vaccines against bovine tuberculosis will contribute to the control of this zoonotic disease. Rationally attenuated Mycobacterium bovis strains generated by knockout of virulence genes are promising candidate vaccines. However, to be effective, these candidate vaccines should at least maintain the immunological properties of their virulent parental M. bovis strains. Therefore, the aim of this study was to obtain an M. bovis strain deleted in the mce2 genes and evaluate the effect of the mutation on the immunological profile elicited by the bacteria in cattle. We showed that the activation of CD4+ T cells in cattle inoculated with the mutant strain was equivalent to that in animals inoculated with the parental strain. Moreover, after in vitro stimulation, peripheral blood mononuclear cells from animals inoculated with the mutant produced higher levels of mRNA Th-1 cytokines than the parental strain. Therefore, these results indicate that the mce2 mutant is a promising candidate vaccine against bovine tuberculosis. 
653 0 |a ANIMAL 
653 0 |a ANIMALIA 
653 0 |a ANIMALS 
653 0 |a ANTIGENS, BACTERIAL 
653 0 |a BACTERIAL ANTIGEN 
653 0 |a BACTERIAL PROTEIN 
653 0 |a BACTERIAL PROTEINS 
653 0 |a BACTERIAL STRAIN 
653 0 |a BCG VACCINE 
653 0 |a BOS 
653 0 |a BOVINAE 
653 0 |a BOVINE TUBERCULOSIS 
653 0 |a CATTLE 
653 0 |a CD4+ T LYMPHOCYTE 
653 0 |a CD4-POSITIVE T-LYMPHOCYTES 
653 0 |a CD8+ T LYMPHOCYTE 
653 0 |a CD8-POSITIVE T-LYMPHOCYTES 
653 0 |a CELL CULTURE 
653 0 |a CELLS, CULTURED 
653 0 |a CYTOKINE 
653 0 |a CYTOKINES 
653 0 |a GENE 
653 0 |a GENE DELETION 
653 0 |a GENE INACTIVATION 
653 0 |a GENE KNOCKOUT TECHNIQUES 
653 0 |a GENE MUTATION 
653 0 |a GENETICS 
653 0 |a HOST PATHOGEN INTERACTION 
653 0 |a HOST-PATHOGEN INTERACTIONS 
653 0 |a IMMUNE RESPONSE 
653 0 |a IMMUNOLOGY 
653 0 |a IN VITRO STUDY 
653 0 |a INOCULATION 
653 0 |a LEUKOCYTES, MONONUCLEAR 
653 0 |a LYMPHOCYTE ACTIVATION 
653 0 |a MCE2 GENE 
653 0 |a MESSENGER RNA 
653 0 |a METABOLISM 
653 0 |a MONONUCLEAR CELL 
653 0 |a MYCOBACTERIUM BOVIS 
653 0 |a PERIPHERAL BLOOD MONONUCLEAR CELL 
653 0 |a POLYMERASE CHAIN REACTION 
653 0 |a RNA, MESSENGER 
653 0 |a TH1 CELL 
653 0 |a TUBERCULOSIS VACCINES 
653 0 |a TUBERCULOSIS, BOVINE 
700 1 |9 70699  |a Blanco, Federico Carlos 
700 1 |9 49057  |a Soria, Marcelo Abel 
700 1 |9 69464  |a Gravisaco, María José 
700 1 |a Bianco, María Verónica  |9 48056 
700 1 |a Meikle, Virginia  |9 48057 
700 1 |a Garbaccio, Sergio  |9 44669 
700 1 |a Vagnoni, Lucas  |9 48058 
700 1 |a Cataldi, Angel Adrián  |9 38812 
700 1 |9 48059  |a Bigi, Fabiana 
773 |t Journal of Biomedicine and Biotechnology  |g vol.2012 (2012), p.1-8 
856 |u http://ri.agro.uba.ar/files/intranet/articulo/2012blanco1.pdf  |i En reservorio  |q application/pdf  |f 2012blanco1  |x MIGRADOS2018 
856 |u http://www.hindawi.com/  |x MIGRADOS2018  |z LINK AL EDITOR 
942 0 0 |c ARTICULO 
942 0 0 |c ENLINEA 
976 |a AAG 

Ejemplares similares