Isolation, purification and some properties of an extracellular phbv depolymerase from Streptomyces SP. SSM 5670

Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates [PHAs], showed a high activity of the extracellular poly [3-hydrox butyrate-co-3-hydroxyvalerate] [PHBV] depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The ext...

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Detalles Bibliográficos
Autor principal: Yashchuk, O.
Otros Autores: Miyazaki, Silvia Susana, Hermida, E. B.
Formato: Artículo
Lenguaje:Español
Materias:
2 DIETHYLAMINOETHANOL
ANION EXCHANGE
BACTERIUM ISOLATION
COMPOST
ENZYME ACTIVITY
ENZYME DEGRADATION
ENZYME ELECTROPHORESIS
ENZYME ISOLATION
ENZYME PURIFICATION
GEL FILTRATION
ION EXCHANGE CHROMATOGRAPHY
MOLECULAR WEIGHT
NONHUMAN
PHBV DEPOLYMERASE
POLY [3 HYDROXYBUTYRATE CO 3 HYDROXYVALERATE] DEPOLYMERASE
POLYACRYLAMIDE GEL ELECTROPHORESIS
POLYMER
POLYSORBATE 80
ROOM TEMPERATURE
SOIL
STREPTOMYCES
STREPTOMYCES SP.
ULTRAFILTRATION
UNCLASSIFIED DRUG
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2014yashchuk.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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100 1 |a Yashchuk, O.  |9 71620 
245 0 0 |a Isolation, purification and some properties of an extracellular phbv depolymerase from Streptomyces SP. SSM 5670 
520 |a Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates [PHAs], showed a high activity of the extracellular poly [3-hydrox butyrate-co-3-hydroxyvalerate] [PHBV] depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5 percent. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30°C, the activity was lost at temperatures above 45°C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature. 
650 |2 Agrovoc  |9 26 
653 0 |a 2 DIETHYLAMINOETHANOL 
653 0 |a ANION EXCHANGE 
653 0 |a BACTERIUM ISOLATION 
653 0 |a COMPOST 
653 0 |a ENZYME ACTIVITY 
653 0 |a ENZYME DEGRADATION 
653 0 |a ENZYME ELECTROPHORESIS 
653 0 |a ENZYME ISOLATION 
653 0 |a ENZYME PURIFICATION 
653 0 |a GEL FILTRATION 
653 0 |a ION EXCHANGE CHROMATOGRAPHY 
653 0 |a MOLECULAR WEIGHT 
653 0 |a NONHUMAN 
653 0 |a PHBV DEPOLYMERASE 
653 0 |a POLY [3 HYDROXYBUTYRATE CO 3 HYDROXYVALERATE] DEPOLYMERASE 
653 0 |a POLYACRYLAMIDE GEL ELECTROPHORESIS 
653 0 |a POLYMER 
653 0 |a POLYSORBATE 80 
653 0 |a ROOM TEMPERATURE 
653 0 |a SOIL 
653 0 |a STREPTOMYCES 
653 0 |a STREPTOMYCES SP. 
653 0 |a ULTRAFILTRATION 
653 0 |a UNCLASSIFIED DRUG 
700 |a Miyazaki, Silvia Susana  |9 4827 
700 1 |a Hermida, E. B.  |9 71621 
773 |t Asian Journal of Microbiology, Biotechnology and Environmental Sciences  |g vol.16, no.1 (2014), p.29-33 
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