The Arabidopsis B-BOX protein BBX25 interacts with HY5, negatively regulating BBX22 expression to suppress seedling photomorphogenesis

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ELONGATED HYPOCOTYL5 [HY5] is a basic domain/leucine zipper [bZIP] transcription factor, central for the regulation of seedling photomorphogenesis. Here, we identified a B-BOX [BBX]-containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an interacting partner of HY5, which has been previously found to...

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Detalles Bibliográficos
Otros Autores: Gangappa, Sreeramaiah N., Crocco, Carlos Daniel, Johansson, Henrik, Datta, Sourav, Hettiarachchi, Chamari, Holm, Magnus, Botto, Javier Francisco
Formato: Artículo
Lenguaje:Inglés
Materias:
ARABIDOPSIS PROTEIN
BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR
BBX24 PROTEIN, ARABIDOPSIS
BBX25 PROTEIN, ARABIDOPSIS
CONSTITUTIVE PHOTOMORPHOGENIC 1 PROTEIN, ARABIDOPSIS
HY5 PROTEIN, ARABIDOPSIS
LZF1 PROTEIN, ARABIDOPSIS
NUCLEAR PROTEIN
PHOTOPROTEIN
REPRESSOR PROTEIN
TRANSCRIPTION FACTOR
ARABIDOPSIS
BIOLOGICAL MODEL
FLUORESCENCE MICROSCOPY
GENE EXPRESSION REGULATION
GENETICS
GROWTH, DEVELOPMENT AND AGING
IMMUNOBLOTTING
LIGHT
METABOLISM
PLANT GROWTH
PROTEIN BINDING
PROTOPLAST
RADIATION EXPOSURE
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
SEEDLING
TWO HYBRID SYSTEM
ARABIDOPSIS PROTEINS
BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTORS
GENE EXPRESSION REGULATION, DEVELOPMENTAL
GENE EXPRESSION REGULATION, PLANT
HYPOCOTYL
LUMINESCENT PROTEINS
MICROSCOPY, FLUORESCENCE
MODELS, GENETIC
NUCLEAR PROTEINS
PROTOPLASTS
REPRESSOR PROTEINS
TRANSCRIPTION FACTORS
TWO-HYBRID SYSTEM TECHNIQUES
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2013gangappa.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 0 0 |a The Arabidopsis B-BOX protein BBX25 interacts with HY5, negatively regulating BBX22 expression to suppress seedling photomorphogenesis 
520 |a ELONGATED HYPOCOTYL5 [HY5] is a basic domain/leucine zipper [bZIP] transcription factor, central for the regulation of seedling photomorphogenesis. Here, we identified a B-BOX [BBX]-containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an interacting partner of HY5, which has been previously found to physically interact with CONSTITUTIVE PHOTOMORPHOGENIC1 [COP1]. BBX25 physically interacts with HY5 both in vitro and in vivo. By physiological and genetic approaches, we showed that BBX25 is a negative regulator of seedling photomorphogenesis. BBX25 and its homolog BBX24 regulate deetiolation processes and hypocotyl shade avoidance response in an additive manner. Moreover, genetic relationships of bbx25 and bbx24 with hy5 and cop1 revealed that BBX25 and BBX24 additively enhance COP1 and suppress HY5 functions. BBX25 accumulates in a light-dependent manner and undergoes COP1-mediated degradation in dark and light conditions. Furthermore, a protoplast cotransfection assay showed that BBX24 and BBX25 repress BBX22 expression by interfering with HY5 transcriptional activity. As HY5 binds to the BBX22 promoter and promotes its expression, our results identify a direct mechanism through which the expression of BBX22 is regulated. We suggest that BBX25 and BBX24 function as transcriptional corepressors, probably by forming inactive heterodimers with HY5, downregulating BBX22 expression for the fine-tuning of light-mediated seedling development. 
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700 1 |a Hettiarachchi, Chamari  |9 72636 
700 1 |9 69361  |a Holm, Magnus 
700 1 |9 65609  |a Botto, Javier Francisco 
773 |t The Plant Cell  |g vol.25, no.4 (2013), p.1243-1257 
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900 |a ^aGangappa^bS.N.^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aCrocco^bC.D.^tInstituto de Investigaciones Fisiológicas y Ecologicas Vinculadas a la Agricultura, Universidad de Buenos Aires y Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires 1417, Argentina 
900 |a ^aJohansson^bH.^tDepartment of Biotechnology, National Institute of Technology, Durgapur 713209, West Bengal, India 
900 |a ^aDatta^bS.^tDepartment of Plant Physiology, Justus-Liebig University, Sencken bergstr. 3, 35390 Giessen, Germany 
900 |a ^aHettiarachchi^bC.^tDepartment of Plant Sciences, University of Oxford, Oxford 0X1 3RB, United Kingdom 
900 |a ^aHolm^bM.^tDepartment of Chemistry, Colombo University, Colombo 03, Sri Lanka 
900 |a ^aBotto^bJ.F. 
900 |a ^aGangappa, S.N^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aGangappa, S.N^tDepartment of Biotechnology, National Institute of Technology, Durgapur 713209, West Bengal, India 
900 |a ^aCrocco, C.D^tInstituto de Investigaciones Fisiológicas y Ecologicas Vinculadas a la Agricultura, Universidad de Buenos Aires y Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires 1417, Argentina 
900 |a ^aJohansson, H^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aJohansson, H^tDepartment of Plant Physiology, Justus-Liebig University, Sencken bergstr^t3, 35390 Giessen, Germany 
900 |a ^aDatta, S^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aDatta, S^tDepartment of Plant Sciences, University of Oxford, Oxford 0X1 3RB, United Kingdom 
900 |a ^aHettiarachchi, C^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aHettiarachchi, C^tDepartment of Chemistry, Colombo University, Colombo 03, Sri Lanka 
900 |a ^aHolm, M^tDepartment of Biological and Environmental Sciences, Gothenburg University, Gothenburg SE 40530, Sweden 
900 |a ^aBotto, J.F^tInstituto de Investigaciones Fisiológicas y Ecologicas Vinculadas a la Agricultura, Universidad de Buenos Aires y Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires 1417, Argentina 
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900 |a ELONGATED HYPOCOTYL5 [HY5] is a basic domain/leucine zipper [bZIP] transcription factor, central for the regulation of seedling photomorphogenesis. Here, we identified a B-BOX [BBX]-containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an interacting partner of HY5, which has been previously found to physically interact with CONSTITUTIVE PHOTOMORPHOGENIC1 [COP1]. BBX25 physically interacts with HY5 both in vitro and in vivo. By physiological and genetic approaches, we showed that BBX25 is a negative regulator of seedling photomorphogenesis. BBX25 and its homolog BBX24 regulate deetiolation processes and hypocotyl shade avoidance response in an additive manner. Moreover, genetic relationships of bbx25 and bbx24 with hy5 and cop1 revealed that BBX25 and BBX24 additively enhance COP1 and suppress HY5 functions. BBX25 accumulates in a light-dependent manner and undergoes COP1-mediated degradation in dark and light conditions. Furthermore, a protoplast cotransfection assay showed that BBX24 and BBX25 repress BBX22 expression by interfering with HY5 transcriptional activity. As HY5 binds to the BBX22 promoter and promotes its expression, our results identify a direct mechanism through which the expression of BBX22 is regulated. We suggest that BBX25 and BBX24 function as transcriptional corepressors, probably by forming inactive heterodimers with HY5, downregulating BBX22 expression for the fine-tuning of light-mediated seedling development. 
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