Methods for detection of Alternaria padwickii in rice seeds

The fungus Alternaria padwickii has been frequently detected in seed tests of rice collected from commercial crops in Corrientes Province, Argentina. This pathogen causes germination inhibition, seedling death or spotted grains and is the causal agent of Alternaria leaf spot. The pathogen survives a...

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Autor principal: Gutiérrez, Susana Alejandra
Otros Autores: Carmona, Marcelo Aníbal, Reis, Erlei Melo
Formato: Artículo
Lenguaje:Inglés
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Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2010Gutierrez.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
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520 |a The fungus Alternaria padwickii has been frequently detected in seed tests of rice collected from commercial crops in Corrientes Province, Argentina. This pathogen causes germination inhibition, seedling death or spotted grains and is the causal agent of Alternaria leaf spot. The pathogen survives as mycelia and sclerotia on seeds, plant debris and soil. Four detection methods were compared in laboratory tests, to select the best for a quick identification of the fungus in seeds. The methods were [i] Blotter Test [ii] Potato glucose agar, [iii] Bean agar [BA] and [iv] Malt extract agar. Twenty seed samples of different varieties of rice collected from Empedrado, Goya, Itá Ibaté, La Cruz, Mercedes, Paso de los Libres and Perugorría localities [Corrientes, Argentina], were analyzed in the assays. The anova test and the Tukey multiple range test were applied on the data to compare the A. padwickii incidence among the varieties and detection methods. BA method was found more sensitive than other methods for A. padwickii. The incidence values ranged from 3.6 to 76 percent. The statistical analysis demonstrated that the BA method was the most efficient for the detection of seed pathogens, and it could be useful in studies of transmission and chemical control. 
653 0 |a ORYZA SATIVA 
653 0 |a PATHOGEN 
653 0 |a SEED HEALTH TESTING 
653 0 |a SEEDBORNE PATHOGENS 
653 0 |a BIOASSAY 
653 0 |a CHEMICAL CONTROL 
653 0 |a COMMERCIAL SPECIES 
653 0 |a DISEASE INCIDENCE 
653 0 |a DISEASE TRANSMISSION 
653 0 |a FUNGAL DISEASE 
653 0 |a HOST-PATHOGEN INTERACTION 
653 0 |a LABORATORY METHOD 
653 0 |a MONOCOTYLEDON 
653 0 |a RICE 
653 0 |a STATISTICAL ANALYSIS 
653 0 |a SURVIVAL 
653 0 |a ARGENTINA 
653 0 |a CORRIENTES 
653 0 |a ALTERNARIA 
653 0 |a ALTERNARIA PADWICKII 
653 0 |a FUNGI 
653 0 |a ORYZA SATIVA 
653 0 |a SOLANUM TUBEROSUM 
700 1 |9 3109  |a Carmona, Marcelo Aníbal 
700 1 |9 7504  |a Reis, Erlei Melo 
773 |t Journal of Phytopathology  |g vol.158, no.7-8 (2010), p.523-526 
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900 |a ^aGutiérrez^bS.A.^tCátedra de Fitopatología, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131 [3400], Corrientes, Argentina 
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900 |a ^aReis^bE.M.^tFitopatología, Facultade de Agronomia e Veterinaria, Universidad de Passo Fundo, Caixa Postal 611, 99001-970 Passo Fundo, Brazil 
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900 |a ORYZA SATIVA 
900 |a PATHOGEN 
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900 |a SEEDBORNE PATHOGENS 
900 |a BIOASSAY 
900 |a CHEMICAL CONTROL 
900 |a COMMERCIAL SPECIES 
900 |a DISEASE INCIDENCE 
900 |a DISEASE TRANSMISSION 
900 |a FUNGAL DISEASE 
900 |a HOST-PATHOGEN INTERACTION 
900 |a LABORATORY METHOD 
900 |a MONOCOTYLEDON 
900 |a RICE 
900 |a STATISTICAL ANALYSIS 
900 |a SURVIVAL 
900 |a ARGENTINA 
900 |a CORRIENTES 
900 |a ALTERNARIA 
900 |a ALTERNARIA PADWICKII 
900 |a FUNGI 
900 |a ORYZA SATIVA 
900 |a SOLANUM TUBEROSUM 
900 |a The fungus Alternaria padwickii has been frequently detected in seed tests of rice collected from commercial crops in Corrientes Province, Argentina. This pathogen causes germination inhibition, seedling death or spotted grains and is the causal agent of Alternaria leaf spot. The pathogen survives as mycelia and sclerotia on seeds, plant debris and soil. Four detection methods were compared in laboratory tests, to select the best for a quick identification of the fungus in seeds. The methods were [i] Blotter Test [ii] Potato glucose agar, [iii] Bean agar [BA] and [iv] Malt extract agar. Twenty seed samples of different varieties of rice collected from Empedrado, Goya, Itá Ibaté, La Cruz, Mercedes, Paso de los Libres and Perugorría localities [Corrientes, Argentina], were analyzed in the assays. The anova test and the Tukey multiple range test were applied on the data to compare the A. padwickii incidence among the varieties and detection methods. BA method was found more sensitive than other methods for A. padwickii. The incidence values ranged from 3.6 to 76 percent. The statistical analysis demonstrated that the BA method was the most efficient for the detection of seed pathogens, and it could be useful in studies of transmission and chemical control. 
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