Cytotoxic effects of argentinean plant extracts on tumour and normal cell lines

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In the search for possible new anti-cancer agents, we investigated the effects of 75 aqueous and methanol extracts from 41 Argentinean plant species. The effect in cell growth was evaluated in the LM2 mammary adenocarcinoma cells. In a second stage, the highly active selected extracts were assayed i...

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Detalles Bibliográficos
Otros Autores: Mamone, L., Di Venosa, G., Valla, Juan José ( - 2020), Rodríguez, L., Gándara, L., Batlle, A., Heinrich, M., Juarranz, A., Sanz Rodriguez, F., Casas, A.
Formato: Artículo
Lenguaje:Inglés
Materias:
ALKALOIDS
CELL LINES
MICROTUBULES
PLANT EXTRACT
AMARANTHUS QUITENSIS EXTRACT
ANTINEOPLASTIC AGENT
BROMELIA BALANSAE EXTRACT
COLLAEA ARGENTINA EXTRACT
COLLETIA PARADOXA EXTRACT
COMBRETUM FRUTICOSUM EXTRACT
COMMELINA ERECTA EXTRACT
EPHEDRA TWEEDIANA EXTRACT
ERYTHRINA FALCATA EXTRACT
IOCHROMA AUSTRALE EXTRACT
IPOMOEA BONARIENSIS EXTRACT
JACARANDA MIMOSIFOLIA EXTRACT
LANTANA CAMARA EXTRACT
OXALIS LATIFOLIA EXTRACT
PARKINSONIA ACULEATA EXTRACT
PAVONIA SEPIUM EXTRACT
PRUNUS SUBCORIACEA EXTRACT
PYROSTEGIA VENUSTA EXTRACT
RIVINA HUMILIS EXTRACT
RUELLIA BRITTONIANA EXTRACT
SCUTIA BUXIFOLIA EXTRACT
SOLANUM AMYGDALIFOLIUM EXTRACT
SOLANUM CHACOENSE EXTRACT
SOLANUM SISYMBRIIFOLIUM EXTRACT
SOLANUM VERBASCIFOLIUM EXTRACT
TABEBUIA IMPETIGINOSA EXTRACT
UNCLASSIFIED DRUG
UNINDEXED DRUG
VINBLASTINE
XANTHIUM CAVANILLESII EXTRACT
AMARANTHUS QUITENSIS
ANIMAL CELL
APOPTOSIS
ARGENTINA
BROMELIA BALANSAE
CANCER INHIBITION
CELL GROWTH
CELL STRUCTURE
CELL VIABILITY
COLLAEA ARGENTINA
COLLETIA PARADOXA
COMBRETUM FRUTICOSUM
COMMELINA ERECTA
CYTOTOXICITY
EPHEDRA TWEEDIANA
ERYTHRINA FALCATA
FLOWER
HUMAN
HUMAN CELL
IC 50
IOCHROMA AUSTRALE
IPOMOEA BONARIENSIS
KERATINOCYTE
MEDICINAL PLANT
MELANOMA CELL
MICROTUBULE
NONHUMAN
OXALIS LATIFOLIA
PARKINSONIA ACULEATA
PAVONIA SEPIUM
PLANT LEAF
PRUNUS SUBCORIACEA
PYROSTEGIA VENUSTA
RIVINA HUMILIS
RUELLIA BRITTONIANA
SCUTIA BUXIFOLIA
SOLANUM CHACOENSE
SOLANUM SISYMBRIIFOLIUM
SOLANUM VERBASCIFOLIUM
TABEBUIA IMPETIGINOSA
TUMOR CELL
XANTHIUM CAVANILLESII
ANTINEOPLASTIC AGENTS
CELL LINE, TUMOR
CELL SURVIVAL
FLOWERS
HUMANS
INHIBITORY CONCENTRATION 50
IPOMOEA
LAMIACEAE
PHASEOLUS
PHYSALIS
PLANT EXTRACTS
PLANT LEAVES
TUBULIN
IOCHROMA
JACARANDA MIMOSIFOLIA
MURINAE
SOLANUM
SOLANUM AMYGDALIFOLIUM
VINCA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2011Mamone.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Cytotoxic effects of argentinean plant extracts on tumour and normal cell lines 
520 |a In the search for possible new anti-cancer agents, we investigated the effects of 75 aqueous and methanol extracts from 41 Argentinean plant species. The effect in cell growth was evaluated in the LM2 mammary adenocarcinoma cells. In a second stage, the highly active selected extracts were assayed in 3 other tumour cell lines: melanoma B16, bladder MB49 and lung A549; and 3 normal cell lines: mammary Hb4a and keratinocytes PAM212 and HaCat. Eight methanol extracts were found to be highly cytotoxic: Collaea argentina leaf, Iochroma australe leaf, Ipomoea bonariensis flower, Jacaranda mimosifolia flower, Solanum amygdalifolium flower, Solanum chacoense leaf, Solanum sisymbriifolium flower and Solanum verbascifolium flower. However, extract inhibition on cell growth was highly dependent on cell type. In general, except for the highly resistant cell lines, the inhibitory concentrations 50 percent were in the range of 10-150 ug/ml The eight extracts highly inhibited cell growth in a concentration-dependent manner, and in general the methanol extracts were always more active than the aqueous. Murine cells appear to be more sensitive than human cells to the cytotoxic action of the plant extracts. The human melanoma B16 line was the most resistant to four of the extracts. In terms of selectivity, S. verbascifolium was the species which showed most selectivity for tumour cells. Overall, this is one of the first studies focusing on southern South American native plants and their biological effects. Since some species of 5 genera analyzed have been reported to possess different degrees of alkaloid content, we examined microtubule structures after extract treatments. The eight extracts induced destabilization, condensation and aggregation of microtubules in LM2 cells, although no depolarization, typical of Vinca alkaloids damage was observed. In a near future, antitumour activity of purified fractions of the extracts administered at non-toxic doses will be assayed in transplantable murine tumour models. 
653 0 |a ALKALOIDS 
653 0 |a CELL LINES 
653 0 |a MICROTUBULES 
653 0 |a PLANT EXTRACT 
653 0 |a AMARANTHUS QUITENSIS EXTRACT 
653 0 |a ANTINEOPLASTIC AGENT 
653 0 |a BROMELIA BALANSAE EXTRACT 
653 0 |a COLLAEA ARGENTINA EXTRACT 
653 0 |a COLLETIA PARADOXA EXTRACT 
653 0 |a COMBRETUM FRUTICOSUM EXTRACT 
653 0 |a COMMELINA ERECTA EXTRACT 
653 0 |a EPHEDRA TWEEDIANA EXTRACT 
653 0 |a ERYTHRINA FALCATA EXTRACT 
653 0 |a IOCHROMA AUSTRALE EXTRACT 
653 0 |a IPOMOEA BONARIENSIS EXTRACT 
653 0 |a JACARANDA MIMOSIFOLIA EXTRACT 
653 0 |a LANTANA CAMARA EXTRACT 
653 0 |a OXALIS LATIFOLIA EXTRACT 
653 0 |a PARKINSONIA ACULEATA EXTRACT 
653 0 |a PAVONIA SEPIUM EXTRACT 
653 0 |a PRUNUS SUBCORIACEA EXTRACT 
653 0 |a PYROSTEGIA VENUSTA EXTRACT 
653 0 |a RIVINA HUMILIS EXTRACT 
653 0 |a RUELLIA BRITTONIANA EXTRACT 
653 0 |a SCUTIA BUXIFOLIA EXTRACT 
653 0 |a SOLANUM AMYGDALIFOLIUM EXTRACT 
653 0 |a SOLANUM CHACOENSE EXTRACT 
653 0 |a SOLANUM SISYMBRIIFOLIUM EXTRACT 
653 0 |a SOLANUM VERBASCIFOLIUM EXTRACT 
653 0 |a TABEBUIA IMPETIGINOSA EXTRACT 
653 0 |a UNCLASSIFIED DRUG 
653 0 |a UNINDEXED DRUG 
653 0 |a VINBLASTINE 
653 0 |a XANTHIUM CAVANILLESII EXTRACT 
653 0 |a AMARANTHUS QUITENSIS 
653 0 |a ANIMAL CELL 
653 0 |a APOPTOSIS 
653 0 |a ARGENTINA 
653 0 |a BROMELIA BALANSAE 
653 0 |a CANCER INHIBITION 
653 0 |a CELL GROWTH 
653 0 |a CELL STRUCTURE 
653 0 |a CELL VIABILITY 
653 0 |a COLLAEA ARGENTINA 
653 0 |a COLLETIA PARADOXA 
653 0 |a COMBRETUM FRUTICOSUM 
653 0 |a COMMELINA ERECTA 
653 0 |a CYTOTOXICITY 
653 0 |a EPHEDRA TWEEDIANA 
653 0 |a ERYTHRINA FALCATA 
653 0 |a FLOWER 
653 0 |a HUMAN 
653 0 |a HUMAN CELL 
653 0 |a IC 50 
653 0 |a IOCHROMA AUSTRALE 
653 0 |a IPOMOEA BONARIENSIS 
653 0 |a KERATINOCYTE 
653 0 |a MEDICINAL PLANT 
653 0 |a MELANOMA CELL 
653 0 |a MICROTUBULE 
653 0 |a NONHUMAN 
653 0 |a OXALIS LATIFOLIA 
653 0 |a PARKINSONIA ACULEATA 
653 0 |a PAVONIA SEPIUM 
653 0 |a PLANT LEAF 
653 0 |a PRUNUS SUBCORIACEA 
653 0 |a PYROSTEGIA VENUSTA 
653 0 |a RIVINA HUMILIS 
653 0 |a RUELLIA BRITTONIANA 
653 0 |a SCUTIA BUXIFOLIA 
653 0 |a SOLANUM CHACOENSE 
653 0 |a SOLANUM SISYMBRIIFOLIUM 
653 0 |a SOLANUM VERBASCIFOLIUM 
653 0 |a TABEBUIA IMPETIGINOSA 
653 0 |a TUMOR CELL 
653 0 |a XANTHIUM CAVANILLESII 
653 0 |a ANTINEOPLASTIC AGENTS 
653 0 |a CELL LINE, TUMOR 
653 0 |a CELL SURVIVAL 
653 0 |a FLOWERS 
653 0 |a HUMANS 
653 0 |a INHIBITORY CONCENTRATION 50 
653 0 |a IPOMOEA 
653 0 |a LAMIACEAE 
653 0 |a PHASEOLUS 
653 0 |a PHYSALIS 
653 0 |a PLANT EXTRACTS 
653 0 |a PLANT LEAVES 
653 0 |a TUBULIN 
653 0 |a IOCHROMA 
653 0 |a IPOMOEA 
653 0 |a JACARANDA MIMOSIFOLIA 
653 0 |a MURINAE 
653 0 |a SOLANUM 
653 0 |a SOLANUM AMYGDALIFOLIUM 
653 0 |a VINCA 
700 1 |a Mamone, L.  |9 72085 
700 1 |a Di Venosa, G.  |9 72086 
700 |9 6127  |a Valla, Juan José  |d ( - 2020) 
700 1 |a Rodríguez, L.  |9 67239 
700 1 |a Gándara, L.  |9 72087 
700 1 |a Batlle, A.  |9 72088 
700 1 |a Heinrich, M.  |9 72089 
700 1 |a Juarranz, A.  |9 72090 
700 1 |a Sanz Rodriguez, F.  |9 72091 
700 |a Casas, A.  |9 72092 
773 |t Cellular and Molecular Biology  |g Vol.57, suppl. (2011), p.1487-1499 
856 |u http://ri.agro.uba.ar/files/intranet/articulo/2011Mamone.pdf  |i En reservorio  |q application/pdf  |f 2011Mamone  |x MIGRADOS2018 
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900 |a ^aMamone^bL.^tCentro de Investigaciones sobre Porfirinas y Porfirias [CIPYP], CONICET and Hospital de Clínicas José de San Martín, University of Buenos Aires, Córdoba 2351 1er subsuelo, Ciudad de Buenos Aires, CP1120AAF, Argentina 
900 |a ^aDi Venosa^bG.^tDepartment of Botany, School of Agronomy, University of Buenos Aires, Argentina 
900 |a ^aValla^bJ.J.^tCentre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London and Southern Cross Plant Science, NSW, Australia 
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900 |a ANTINEOPLASTIC AGENT 
900 |a BROMELIA BALANSAE EXTRACT 
900 |a COLLAEA ARGENTINA EXTRACT 
900 |a COLLETIA PARADOXA EXTRACT 
900 |a COMBRETUM FRUTICOSUM EXTRACT 
900 |a COMMELINA ERECTA EXTRACT 
900 |a EPHEDRA TWEEDIANA EXTRACT 
900 |a ERYTHRINA FALCATA EXTRACT 
900 |a IOCHROMA AUSTRALE EXTRACT 
900 |a IPOMOEA BONARIENSIS EXTRACT 
900 |a JACARANDA MIMOSIFOLIA EXTRACT 
900 |a LANTANA CAMARA EXTRACT 
900 |a OXALIS LATIFOLIA EXTRACT 
900 |a PARKINSONIA ACULEATA EXTRACT 
900 |a PAVONIA SEPIUM EXTRACT 
900 |a PRUNUS SUBCORIACEA EXTRACT 
900 |a PYROSTEGIA VENUSTA EXTRACT 
900 |a RIVINA HUMILIS EXTRACT 
900 |a RUELLIA BRITTONIANA EXTRACT 
900 |a SCUTIA BUXIFOLIA EXTRACT 
900 |a SOLANUM AMYGDALIFOLIUM EXTRACT 
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900 |a COMBRETUM FRUTICOSUM 
900 |a COMMELINA ERECTA 
900 |a CYTOTOXICITY 
900 |a EPHEDRA TWEEDIANA 
900 |a ERYTHRINA FALCATA 
900 |a FLOWER 
900 |a HUMAN 
900 |a HUMAN CELL 
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900 |a IOCHROMA AUSTRALE 
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900 |a SCUTIA BUXIFOLIA 
900 |a SOLANUM CHACOENSE 
900 |a SOLANUM SISYMBRIIFOLIUM 
900 |a SOLANUM VERBASCIFOLIUM 
900 |a TABEBUIA IMPETIGINOSA 
900 |a TUMOR CELL 
900 |a XANTHIUM CAVANILLESII 
900 |a ANTINEOPLASTIC AGENTS 
900 |a CELL LINE, TUMOR 
900 |a CELL SURVIVAL 
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900 |a INHIBITORY CONCENTRATION 50 
900 |a IPOMOEA 
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900 |a PHASEOLUS 
900 |a PHYSALIS 
900 |a PLANT EXTRACTS 
900 |a PLANT LEAVES 
900 |a TUBULIN 
900 |a IOCHROMA 
900 |a IPOMOEA 
900 |a JACARANDA MIMOSIFOLIA 
900 |a MURINAE 
900 |a SOLANUM 
900 |a SOLANUM AMYGDALIFOLIUM 
900 |a VINCA 
900 |a In the search for possible new anti-cancer agents, we investigated the effects of 75 aqueous and methanol extracts from 41 Argentinean plant species. The effect in cell growth was evaluated in the LM2 mammary adenocarcinoma cells. In a second stage, the highly active selected extracts were assayed in 3 other tumour cell lines: melanoma B16, bladder MB49 and lung A549; and 3 normal cell lines: mammary Hb4a and keratinocytes PAM212 and HaCat. Eight methanol extracts were found to be highly cytotoxic: Collaea argentina leaf, Iochroma australe leaf, Ipomoea bonariensis flower, Jacaranda mimosifolia flower, Solanum amygdalifolium flower, Solanum chacoense leaf, Solanum sisymbriifolium flower and Solanum verbascifolium flower. However, extract inhibition on cell growth was highly dependent on cell type. In general, except for the highly resistant cell lines, the inhibitory concentrations 50 percent were in the range of 10-150 ug/ml The eight extracts highly inhibited cell growth in a concentration-dependent manner, and in general the methanol extracts were always more active than the aqueous. Murine cells appear to be more sensitive than human cells to the cytotoxic action of the plant extracts. The human melanoma B16 line was the most resistant to four of the extracts. In terms of selectivity, S. verbascifolium was the species which showed most selectivity for tumour cells. Overall, this is one of the first studies focusing on southern South American native plants and their biological effects. Since some species of 5 genera analyzed have been reported to possess different degrees of alkaloid content, we examined microtubule structures after extract treatments. The eight extracts induced destabilization, condensation and aggregation of microtubules in LM2 cells, although no depolarization, typical of Vinca alkaloids damage was observed. In a near future, antitumour activity of purified fractions of the extracts administered at non-toxic doses will be assayed in transplantable murine tumour models. 
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