Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol

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The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically o...

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Detalles Bibliográficos
Otros Autores: Galvagno, Miguel Angel, Iannone, Leopoldo J., Bianchi, Jorgelina, Kronberg, María Florencia, Rost, Enrique, Carstens, María R., Cerrutti, Patricia
Formato: Artículo
Lenguaje:Inglés
Materias:
FATTY ACIDS
GLYCEROL
LIPASE
YARROWIA LIPOLYTICA MUTANT
BIODIESEL
CARBON
FATTY ACID
OLIVE OIL
ORGANIC NITROGEN
TRIACYLGLYCEROL LIPASE
BATCH FERMENTATION
BATCH REACTOR
BIOMASS PRODUCTION
CONTROLLED STUDY
ENZYME ACTIVITY
ENZYME SYNTHESIS
FERMENTATION OPTIMIZATION
FUNGUS MUTANT
NONHUMAN
PROCESS OPTIMIZATION
RESPONSE SURFACE METHOD
YARROWIA LIPOLYTICA
BIOFUELS
BIOMASS
BIOREACTORS
CULTURE MEDIA
CULTURE MEDIA, CONDITIONED
DNA, FUNGAL
DNA, INTERGENIC
FERMENTATION
FUNGAL PROTEINS
GENES, FUNGAL
HYPHAE
INDUSTRIAL MICROBIOLOGY
MYCOLOGY
YARROWIA
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2011Galvagno.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol 
246 0 0 |a Optimización de la producción de biomasa usando glicerol crudo, de una cepa mutante de Yarrowia lipolytica con actividad incrementada de lipasa 
520 |a The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media containing biodiesel-derived glycerol as main carbon source. This strain exhibited distinctive morphological and fatty acid profile characteristics, and showed an increased extracellular lipase activity. An organic source of nitrogen and the addition of 1.0 g/l olive oil were necessary for significant lipase production. Plackett-Burman and Central Composite Statistical Designs were employed for screening and optimization of fermentation in shaken flasks cultures, and the maximum values obtained were 16.1 g/l for biomass and 12.2 Units/ml for lipase, respectively. Optimized batch bioprocess was thereafter scaled in aerated bioreactors and the values reached for lipase specific activity after 95 percent of the glycerol had been consumed, were three-fold higher than those obtained in shaken flasks cultures. A sustainable bioprocess to obtain biomass and extracellular lipase activity was attained by maximizing the use of the by-products of biodiesel industry. 
653 0 |a FATTY ACIDS 
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653 0 |a OLIVE OIL 
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653 0 |a BATCH FERMENTATION 
653 0 |a BATCH REACTOR 
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653 0 |a CONTROLLED STUDY 
653 0 |a ENZYME ACTIVITY 
653 0 |a ENZYME SYNTHESIS 
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653 0 |a FUNGUS MUTANT 
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653 0 |a PROCESS OPTIMIZATION 
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653 0 |a YARROWIA LIPOLYTICA 
653 0 |a BIOFUELS 
653 0 |a BIOMASS 
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653 0 |a CULTURE MEDIA, CONDITIONED 
653 0 |a DNA, FUNGAL 
653 0 |a DNA, INTERGENIC 
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700 1 |a Galvagno, Miguel Angel  |9 69520 
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700 1 |a Rost, Enrique  |9 71818 
700 1 |a Carstens, María R.  |9 71819 
700 1 |a Cerrutti, Patricia  |9 71820 
773 |t Revista argentina de microbiología  |g Vol.43, no.3 (2011), p.218-225 
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900 |a ^tOptimización de la producción de biomasa usando glicerol crudo, de una cepa mutante de Yarrowia lipolytica con actividad incrementada de lipasa 
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900 |a ^aGalvagno^bM.A.^tDepartamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Pabellón de Industrias, Ciudad Universitaria, [1428] Buenos Aires, Argentina 
900 |a ^aIannone^bL.J.^tInstituto de Investigaciones Biotecnológicas, CONICET, UNSAM, Av. General Paz 5445, Edificio 24, [1650], Buenos Aires, Argentina 
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900 |a FATTY ACIDS 
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900 |a BIODIESEL 
900 |a CARBON 
900 |a FATTY ACID 
900 |a OLIVE OIL 
900 |a ORGANIC NITROGEN 
900 |a TRIACYLGLYCEROL LIPASE 
900 |a BATCH FERMENTATION 
900 |a BATCH REACTOR 
900 |a BIOMASS PRODUCTION 
900 |a CONTROLLED STUDY 
900 |a ENZYME ACTIVITY 
900 |a ENZYME SYNTHESIS 
900 |a FERMENTATION OPTIMIZATION 
900 |a FUNGUS MUTANT 
900 |a NONHUMAN 
900 |a PROCESS OPTIMIZATION 
900 |a RESPONSE SURFACE METHOD 
900 |a YARROWIA LIPOLYTICA 
900 |a BIOFUELS 
900 |a BIOMASS 
900 |a BIOREACTORS 
900 |a CULTURE MEDIA 
900 |a CULTURE MEDIA, CONDITIONED 
900 |a DNA, FUNGAL 
900 |a DNA, INTERGENIC 
900 |a FERMENTATION 
900 |a FUNGAL PROTEINS 
900 |a GENES, FUNGAL 
900 |a HYPHAE 
900 |a INDUSTRIAL MICROBIOLOGY 
900 |a MYCOLOGY 
900 |a YARROWIA 
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