Disruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina
Plant resistance to necrotrophic fungi is regulated by a complex set of signaling pathways that includes those mediated by the hormones salicylic acid [SA], ethylene [ET], jasmonic acid [JA], and abscisic acid [ABA]. The role of ABA in plant resistance remains controversial, as positive and negative...
| Otros Autores: | , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Español |
| Materias: | |
| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2012SanchezVallet.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 10577cab a22021977a 4500 | ||
|---|---|---|---|
| 001 | AR-BaUFA000258 | ||
| 003 | AR-BaUFA | ||
| 005 | 20220418145043.0 | ||
| 008 | 181208t2012 |||||o|||||00||||spa d | ||
| 999 | |c 46692 |d 46692 | ||
| 022 | |a 0032-0889 | ||
| 024 | |a 10.1104/pp.112.200154 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | 0 | |a Disruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina |
| 520 | |a Plant resistance to necrotrophic fungi is regulated by a complex set of signaling pathways that includes those mediated by the hormones salicylic acid [SA], ethylene [ET], jasmonic acid [JA], and abscisic acid [ABA]. The role of ABA in plant resistance remains controversial, as positive and negative regulatory functions have been described depending on the plant-pathogen interaction analyzed. Here, we show that ABA signaling negatively regulates Arabidopsis [Arabidopsis thaliana] resistance to the necrotrophic fungus Plectosphaerella cucumerina. Arabidopsis plants impaired in ABA biosynthesis, such as the aba1-6 mutant, or in ABA signaling, like the quadruple pyr/pyl mutant [pyr1pyl1pyl2pyl4], were more resistant to P. cucumerina than wild-type plants. In contrast, the hab1-1abi1-2abi2-2 mutant impaired in three phosphatases that negatively regulate ABA signaling displayed an enhanced susceptibility phenotype to this fungus. Comparative transcriptomic analyses of aba1-6 and wild-type plants revealed that the ABA pathway negatively regulates defense genes, many of which are controlled by the SA, JA, or ET pathway. In line with these data, we found that aba1-6 resistance to P. cucumerina was partially compromised when the SA, JA, or ET pathway was disrupted in this mutant. Additionally, in the aba1-6 plants, some genes encoding cell wall-related proteins were misregulated. Fourier transform infrared spectroscopy and biochemical analyses of cell walls from aba1-6 and wild-type plants revealed significant differences in their Fourier transform infrared spectratypes and uronic acid and cellulose contents. All these data suggest that ABA signaling has a complex function in Arabidopsis basal resistance, negatively regulating SA/JA/ET- mediated resistance to necrotrophic fungi. | ||
| 653 | 0 | |a ABSCISIC ACID | |
| 653 | 0 | |a CYCLOPENTANE DERIVATIVE | |
| 653 | 0 | |a ETHYLENE | |
| 653 | 0 | |a ETHYLENE DERIVATIVE | |
| 653 | 0 | |a JASMONIC ACID | |
| 653 | 0 | |a OXYLIPIN | |
| 653 | 0 | |a PHYTOHORMONE | |
| 653 | 0 | |a SALICYLIC ACID | |
| 653 | 0 | |a ARABIDOPSIS | |
| 653 | 0 | |a ASCOMYCETES | |
| 653 | 0 | |a BIOLOGICAL MODEL | |
| 653 | 0 | |a CELL WALL | |
| 653 | 0 | |a CLUSTER ANALYSIS | |
| 653 | 0 | |a DISEASE RESISTANCE | |
| 653 | 0 | |a DRUG EFFECT | |
| 653 | 0 | |a GENE EXPRESSION PROFILING | |
| 653 | 0 | |a GENE EXPRESSION REGULATION | |
| 653 | 0 | |a GENETICS | |
| 653 | 0 | |a IMMUNOLOGY | |
| 653 | 0 | |a INFRARED SPECTROSCOPY | |
| 653 | 0 | |a METABOLISM | |
| 653 | 0 | |a MICROBIOLOGY | |
| 653 | 0 | |a MUTATION | |
| 653 | 0 | |a PHYSIOLOGICAL STRESS | |
| 653 | 0 | |a PHYSIOLOGY | |
| 653 | 0 | |a PLANT DISEASE | |
| 653 | 0 | |a PLANT GENE | |
| 653 | 0 | |a SIGNAL TRANSDUCTION | |
| 653 | 0 | |a ASCOMYCOTA | |
| 653 | 0 | |a CELL WALL | |
| 653 | 0 | |a CLUSTER ANALYSIS | |
| 653 | 0 | |a CYCLOPENTANES | |
| 653 | 0 | |a DISEASE RESISTANCE | |
| 653 | 0 | |a ETHYLENES | |
| 653 | 0 | |a GENE EXPRESSION PROFILING | |
| 653 | 0 | |a GENE EXPRESSION REGULATION, PLANT | |
| 653 | 0 | |a GENES, PLANT | |
| 653 | 0 | |a MODELS, BIOLOGICAL | |
| 653 | 0 | |a OXYLIPINS | |
| 653 | 0 | |a PLANT DISEASES | |
| 653 | 0 | |a PLANT GROWTH REGULATORS | |
| 653 | 0 | |a SALICYLIC ACID | |
| 653 | 0 | |a SIGNAL TRANSDUCTION | |
| 653 | 0 | |a SPECTROSCOPY, FOURIER TRANSFORM INFRARED | |
| 653 | 0 | |a STRESS, PHYSIOLOGICAL | |
| 700 | 1 | |a Sánchez Vallet, Andrea |9 71787 | |
| 700 | 1 | |a López, Gemma |9 71788 | |
| 700 | 1 | |a Ramos, Brisa |9 71789 | |
| 700 | 1 | |a Delgado Cerezo, Magdalena |9 71790 | |
| 700 | 1 | |a Riviere, Marie Pierre |9 71791 | |
| 700 | 1 | |a Llorente, Francisco |9 71792 | |
| 700 | 1 | |9 67318 |a Fernández, Paula Virginia | |
| 700 | 1 | |a Miedes, Eva |9 71793 | |
| 700 | 1 | |a Grant, Murray |9 71795 | |
| 700 | 1 | |a Molina, Antonio |9 70315 | |
| 773 | |t Plant Physiology |g Vol.160, no.4 (2012), p.2109-2124 | ||
| 856 | |u http://ri.agro.uba.ar/files/intranet/articulo/2012SanchezVallet.pdf |i En reservorio |q application/pdf |f 2012SanchezVallet |x MIGRADOS2018 | ||
| 856 | |u http://my.aspb.org/ |x MIGRADOS2018 |z LINK AL EDITOR | ||
| 900 | |a as | ||
| 900 | |a 20131220 | ||
| 900 | |a N | ||
| 900 | |a SCOPUS | ||
| 900 | |a EMBARGO | ||
| 900 | |a a | ||
| 900 | |a s | ||
| 900 | |a ARTICULO | ||
| 900 | |a EN LINEA | ||
| 900 | |a 00320889 | ||
| 900 | |a 10.1104/pp.112.200154 | ||
| 900 | |a ^tDisruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina | ||
| 900 | |a ^aSánchez-Vallet^bA. | ||
| 900 | |a ^aLópez^bG. | ||
| 900 | |a ^aRamos^bB. | ||
| 900 | |a ^aDelgado-Cerezo^bM. | ||
| 900 | |a ^aRiviere^bM.-P. | ||
| 900 | |a ^aLlorente^bF. | ||
| 900 | |a ^aFernández^bP.V. | ||
| 900 | |a ^aMiedes^bE. | ||
| 900 | |a ^aEstevez^bJ.M. | ||
| 900 | |a ^aGrant^bM. | ||
| 900 | |a ^aMolina^bA. | ||
| 900 | |a ^aSánchez Vallet^bA. | ||
| 900 | |a ^aLópez^bG. | ||
| 900 | |a ^aRamos^bB. | ||
| 900 | |a ^aDelgado Cerezo^bM. | ||
| 900 | |a ^aRiviere^bM. P. | ||
| 900 | |a ^aLlorente^bF. | ||
| 900 | |a ^aFernández^bP. V. | ||
| 900 | |a ^aMiedes^bE. | ||
| 900 | |a ^aEstevez^bJ. M. | ||
| 900 | |a ^aGrant^bM. | ||
| 900 | |a ^aMolina^bA. | ||
| 900 | |a ^aSánchez-Vallet^bA.^tCentro de BiotecnologÃa y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, E-28223 Madrid, Spain | ||
| 900 | |a ^aLópez^bG.^tDepartamento de BiotecnologÃa, Escuela Técnica Superior Ingenieros Agrónomos, Universidad Politécnica de Madrid, E-28040 Madrid, Spain | ||
| 900 | |a ^aRamos^bB.^tCatedra de Quimica de Biomoleculas, Departamento de BiologÃa Aplicada y Alimentos, Facultad de Agronomia, Universidad de Buenos Aires, C1417DSE Buenos Aires, Argentina | ||
| 900 | |a ^aDelgado-Cerezo^bM.^tCollege of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, United Kingdom | ||
| 900 | |a ^aRiviere^bM.-P.^tLaboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands | ||
| 900 | |a ^aLlorente^bF. | ||
| 900 | |a ^aFernández^bP.V. | ||
| 900 | |a ^aMiedes^bE. | ||
| 900 | |a ^aEstevez^bJ.M. | ||
| 900 | |a ^aGrant^bM. | ||
| 900 | |a ^aMolina^bA. | ||
| 900 | |a ^tPlant Physiology^cPlant Physiol. | ||
| 900 | |a eng | ||
| 900 | |a 2109 | ||
| 900 | |a ^i | ||
| 900 | |a Vol. 160, no. 4 | ||
| 900 | |a 2124 | ||
| 900 | |a ABSCISIC ACID | ||
| 900 | |a CYCLOPENTANE DERIVATIVE | ||
| 900 | |a ETHYLENE | ||
| 900 | |a ETHYLENE DERIVATIVE | ||
| 900 | |a JASMONIC ACID | ||
| 900 | |a OXYLIPIN | ||
| 900 | |a PHYTOHORMONE | ||
| 900 | |a SALICYLIC ACID | ||
| 900 | |a ARABIDOPSIS | ||
| 900 | |a ASCOMYCETES | ||
| 900 | |a BIOLOGICAL MODEL | ||
| 900 | |a CELL WALL | ||
| 900 | |a CLUSTER ANALYSIS | ||
| 900 | |a DISEASE RESISTANCE | ||
| 900 | |a DRUG EFFECT | ||
| 900 | |a GENE EXPRESSION PROFILING | ||
| 900 | |a GENE EXPRESSION REGULATION | ||
| 900 | |a GENETICS | ||
| 900 | |a IMMUNOLOGY | ||
| 900 | |a INFRARED SPECTROSCOPY | ||
| 900 | |a METABOLISM | ||
| 900 | |a MICROBIOLOGY | ||
| 900 | |a MUTATION | ||
| 900 | |a PHYSIOLOGICAL STRESS | ||
| 900 | |a PHYSIOLOGY | ||
| 900 | |a PLANT DISEASE | ||
| 900 | |a PLANT GENE | ||
| 900 | |a SIGNAL TRANSDUCTION | ||
| 900 | |a ASCOMYCOTA | ||
| 900 | |a CELL WALL | ||
| 900 | |a CLUSTER ANALYSIS | ||
| 900 | |a CYCLOPENTANES | ||
| 900 | |a DISEASE RESISTANCE | ||
| 900 | |a ETHYLENES | ||
| 900 | |a GENE EXPRESSION PROFILING | ||
| 900 | |a GENE EXPRESSION REGULATION, PLANT | ||
| 900 | |a GENES, PLANT | ||
| 900 | |a MODELS, BIOLOGICAL | ||
| 900 | |a OXYLIPINS | ||
| 900 | |a PLANT DISEASES | ||
| 900 | |a PLANT GROWTH REGULATORS | ||
| 900 | |a SALICYLIC ACID | ||
| 900 | |a SIGNAL TRANSDUCTION | ||
| 900 | |a SPECTROSCOPY, FOURIER TRANSFORM INFRARED | ||
| 900 | |a STRESS, PHYSIOLOGICAL | ||
| 900 | |a Plant resistance to necrotrophic fungi is regulated by a complex set of signaling pathways that includes those mediated by the hormones salicylic acid [SA], ethylene [ET], jasmonic acid [JA], and abscisic acid [ABA]. The role of ABA in plant resistance remains controversial, as positive and negative regulatory functions have been described depending on the plant-pathogen interaction analyzed. Here, we show that ABA signaling negatively regulates Arabidopsis [Arabidopsis thaliana] resistance to the necrotrophic fungus Plectosphaerella cucumerina. Arabidopsis plants impaired in ABA biosynthesis, such as the aba1-6 mutant, or in ABA signaling, like the quadruple pyr/pyl mutant [pyr1pyl1pyl2pyl4], were more resistant to P. cucumerina than wild-type plants. In contrast, the hab1-1abi1-2abi2-2 mutant impaired in three phosphatases that negatively regulate ABA signaling displayed an enhanced susceptibility phenotype to this fungus. Comparative transcriptomic analyses of aba1-6 and wild-type plants revealed that the ABA pathway negatively regulates defense genes, many of which are controlled by the SA, JA, or ET pathway. In line with these data, we found that aba1-6 resistance to P. cucumerina was partially compromised when the SA, JA, or ET pathway was disrupted in this mutant. Additionally, in the aba1-6 plants, some genes encoding cell wall-related proteins were misregulated. Fourier transform infrared spectroscopy and biochemical analyses of cell walls from aba1-6 and wild-type plants revealed significant differences in their Fourier transform infrared spectratypes and uronic acid and cellulose contents. All these data suggest that ABA signaling has a complex function in Arabidopsis basal resistance, negatively regulating SA/JA/ET- mediated resistance to necrotrophic fungi. | ||
| 900 | |a 160 | ||
| 900 | |a 4 | ||
| 900 | |a 2012 | ||
| 900 | |a ^cH | ||
| 900 | |a AAG | ||
| 900 | |a AGROVOC | ||
| 900 | |a 2012SanchezVallet | ||
| 900 | |a AAG | ||
| 900 | |a http://ri.agro.uba.ar/files/intranet/articulo/2012SanchezVallet.pdf | ||
| 900 | |a 2012SanchezVallet.pdf | ||
| 900 | |a http://my.aspb.org/ | ||
| 900 | |a http://www.scopus.com/inward/record.url?eid=2-s2.0-84870795367&partnerID=40&md5=35a4287c1a63206c69984846c55906aa | ||
| 900 | |a ^a^b^c^d^e^f^g^h^i | ||
| 900 | |a OS | ||
| 942 | 0 | 0 | |c ARTICULO |2 udc |
| 942 | 0 | 0 | |c ENLINEA |2 udc |