A systemic vaccine based on Escherichia coli O157:H7 bacterial ghosts [BGs] reduces the excretion of E. coli O157:H7 in calves

Cattle are the main reservoir of enterohemorrhagic . Escherichia coli O157:H7, a bacterium that, in humans, causes hemorrhagic colitis and hemolytic uremic syndrome [HUS], a life-threatening disease, especially in children and older people. Therefore, the development of vaccines preventing colonizat...

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Detalles Bibliográficos
Otros Autores: Vilte, Daniel A., Larzábal, Mariano, Mayr, U. B., Garbaccio, Sergio, Gammella, Mariela, Rabinovitz, Bettina C., Delgado, Fernando, Meikle, Virginia, Cantet, Rodolfo Juan Carlos, Lubitz, P., Lubitz, W., Cataldi, Angel Adrián, Mercado, Elsa C.
Formato: Artículo
Lenguaje:Inglés
Materias:
BACTERIAL GHOSTS
CATTLE
E. COLI O157:H7
VACCINE
BACTERIAL VACCINE
IMMUNOGLOBULIN A
IMMUNOGLOBULIN G
ANIMAL EXPERIMENT
ANIMAL TISSUE
BACTERIAL COLONIZATION
BACTERIAL GHOST
CONTROLLED STUDY
CYTOPLASM
ESCHERICHIA COLI
HUMORAL IMMUNITY
IMMUNIZATION
IMMUNOGLOBULIN BLOOD LEVEL
MALE
NONHUMAN
PROTEIN EXPRESSION
SALIVA ANALYSIS
VACCINATION
WESTERN BLOTTING
ANIMALS
ANTIBODIES, BACTERIAL
BACTERIAL SHEDDING
CATTLE DISEASES
ENZYME-LINKED IMMUNOSORBENT ASSAY
ESCHERICHIA COLI INFECTIONS
ESCHERICHIA COLI O157
ESCHERICHIA COLI VACCINES
RANDOM ALLOCATION
ANIMALIA
BACTERIA [MICROORGANISMS]
BOS
BOVINAE
NEGIBACTERIA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012Vilte.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
Descripción
Sumario:Cattle are the main reservoir of enterohemorrhagic . Escherichia coli O157:H7, a bacterium that, in humans, causes hemorrhagic colitis and hemolytic uremic syndrome [HUS], a life-threatening disease, especially in children and older people. Therefore, the development of vaccines preventing colonization of cattle by . E. coli O157:H7 could be a main tool for an HUS control program. In the present study, we evaluated bacterial ghosts [BGs] of . E. coli O157:H7 as an experimental vaccine against this pathogen. BGs are empty envelopes of Gram-negative bacteria, which retain the morphological surface make-up of their living counterparts and are produced by controlled expression of the cloned protein E, which causes loss of all the cytoplasm content. In this work, . E. coli O157:H7 BGs were used for subcutaneous immunization of calves. The vaccinated animals elicited significant levels of BG-specific IgG but not IgA antibodies in serum. Low levels of IgA and IgG antibodies against BGs were detected in saliva from vaccinated animals. Following oral challenge with . E. coli O157:H7, a significant reduction in both the duration and total bacterial shedding was observed in vaccinated calves compared to the nonimmunized group. We demonstrated that systemic vaccination with . E. coli O157 BGs provides protection in a bovine experimental model. Further research is needed to reach a higher mucosal immune response leading to an optimal vaccine.
ISSN:0165-2427

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