A systemic vaccine based on Escherichia coli O157:H7 bacterial ghosts [BGs] reduces the excretion of E. coli O157:H7 in calves

Cattle are the main reservoir of enterohemorrhagic . Escherichia coli O157:H7, a bacterium that, in humans, causes hemorrhagic colitis and hemolytic uremic syndrome [HUS], a life-threatening disease, especially in children and older people. Therefore, the development of vaccines preventing colonizat...

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Detalles Bibliográficos
Otros Autores: Vilte, Daniel A., Larzábal, Mariano, Mayr, U. B., Garbaccio, Sergio, Gammella, Mariela, Rabinovitz, Bettina C., Delgado, Fernando, Meikle, Virginia, Cantet, Rodolfo Juan Carlos, Lubitz, P., Lubitz, W., Cataldi, Angel Adrián, Mercado, Elsa C.
Formato: Artículo
Lenguaje:Inglés
Materias:
BACTERIAL GHOSTS
CATTLE
E. COLI O157:H7
VACCINE
BACTERIAL VACCINE
IMMUNOGLOBULIN A
IMMUNOGLOBULIN G
ANIMAL EXPERIMENT
ANIMAL TISSUE
BACTERIAL COLONIZATION
BACTERIAL GHOST
CONTROLLED STUDY
CYTOPLASM
ESCHERICHIA COLI
HUMORAL IMMUNITY
IMMUNIZATION
IMMUNOGLOBULIN BLOOD LEVEL
MALE
NONHUMAN
PROTEIN EXPRESSION
SALIVA ANALYSIS
VACCINATION
WESTERN BLOTTING
ANIMALS
ANTIBODIES, BACTERIAL
BACTERIAL SHEDDING
CATTLE DISEASES
ENZYME-LINKED IMMUNOSORBENT ASSAY
ESCHERICHIA COLI INFECTIONS
ESCHERICHIA COLI O157
ESCHERICHIA COLI VACCINES
RANDOM ALLOCATION
ANIMALIA
BACTERIA [MICROORGANISMS]
BOS
BOVINAE
NEGIBACTERIA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012Vilte.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a A systemic vaccine based on Escherichia coli O157:H7 bacterial ghosts [BGs] reduces the excretion of E. coli O157:H7 in calves 
520 |a Cattle are the main reservoir of enterohemorrhagic . Escherichia coli O157:H7, a bacterium that, in humans, causes hemorrhagic colitis and hemolytic uremic syndrome [HUS], a life-threatening disease, especially in children and older people. Therefore, the development of vaccines preventing colonization of cattle by . E. coli O157:H7 could be a main tool for an HUS control program. In the present study, we evaluated bacterial ghosts [BGs] of . E. coli O157:H7 as an experimental vaccine against this pathogen. BGs are empty envelopes of Gram-negative bacteria, which retain the morphological surface make-up of their living counterparts and are produced by controlled expression of the cloned protein E, which causes loss of all the cytoplasm content. In this work, . E. coli O157:H7 BGs were used for subcutaneous immunization of calves. The vaccinated animals elicited significant levels of BG-specific IgG but not IgA antibodies in serum. Low levels of IgA and IgG antibodies against BGs were detected in saliva from vaccinated animals. Following oral challenge with . E. coli O157:H7, a significant reduction in both the duration and total bacterial shedding was observed in vaccinated calves compared to the nonimmunized group. We demonstrated that systemic vaccination with . E. coli O157 BGs provides protection in a bovine experimental model. Further research is needed to reach a higher mucosal immune response leading to an optimal vaccine. 
653 0 |a BACTERIAL GHOSTS 
653 0 |a CATTLE 
653 0 |a E. COLI O157:H7 
653 0 |a VACCINE 
653 0 |a BACTERIAL VACCINE 
653 0 |a IMMUNOGLOBULIN A 
653 0 |a IMMUNOGLOBULIN G 
653 0 |a ANIMAL EXPERIMENT 
653 0 |a ANIMAL TISSUE 
653 0 |a BACTERIAL COLONIZATION 
653 0 |a BACTERIAL GHOST 
653 0 |a CONTROLLED STUDY 
653 0 |a CYTOPLASM 
653 0 |a ESCHERICHIA COLI 
653 0 |a HUMORAL IMMUNITY 
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653 0 |a MALE 
653 0 |a NONHUMAN 
653 0 |a PROTEIN EXPRESSION 
653 0 |a SALIVA ANALYSIS 
653 0 |a VACCINATION 
653 0 |a WESTERN BLOTTING 
653 0 |a ANIMALS 
653 0 |a ANTIBODIES, BACTERIAL 
653 0 |a BACTERIAL SHEDDING 
653 0 |a CATTLE DISEASES 
653 0 |a ENZYME-LINKED IMMUNOSORBENT ASSAY 
653 0 |a ESCHERICHIA COLI INFECTIONS 
653 0 |a ESCHERICHIA COLI O157 
653 0 |a ESCHERICHIA COLI VACCINES 
653 0 |a RANDOM ALLOCATION 
653 0 |a ANIMALIA 
653 0 |a BACTERIA [MICROORGANISMS] 
653 0 |a BOS 
653 0 |a BOVINAE 
653 0 |a NEGIBACTERIA 
700 1 |9 70008  |a Vilte, Daniel A. 
700 1 |9 70009  |a Larzábal, Mariano 
700 1 |a Mayr, U. B.  |9 70010 
700 1 |9 44669  |a Garbaccio, Sergio 
700 1 |9 70011  |a Gammella, Mariela 
700 1 |9 70012  |a Rabinovitz, Bettina C. 
700 1 |9 69467  |a Delgado, Fernando 
700 1 |9 48057  |a Meikle, Virginia 
700 1 |9 12817  |a Cantet, Rodolfo Juan Carlos 
700 1 |a Lubitz, P.  |9 70013 
700 1 |a Lubitz, W.  |9 70014 
700 1 |9 38812  |a Cataldi, Angel Adrián 
700 1 |9 70015  |a Mercado, Elsa C. 
773 |t Veterinary Immunology and Immunopathology  |g Vol.146, no.2 (2012), p.169-176 
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900 |a ^aMayr^bU.B. 
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900 |a ^aLubitz^bW. 
900 |a ^aCataldi^bA. A. 
900 |a ^aMercado^bE. C. 
900 |a ^aVilte^bD.A.^tInstituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria [INTA], Nicolás Repetto y De los Reseros, 1686 Hurlingham, Argentina 
900 |a ^aLarzábal^bM.^tInstituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria [INTA], Nicolás Repetto y De los Reseros, 1686 Hurlingham, Argentina 
900 |a ^aMayr^bU.B.^tBIRD-C GmbH and CoKEG, Haupstrasse 88, 3420 Kritzendorf, Austria 
900 |a ^aGarbaccio^bS.^tDepartamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires - CONICET, Av. San Martín 4453, 1417 Buenos Aires, Argentina 
900 |a ^aGammella^bM. 
900 |a ^aRabinovitz^bB.C. 
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900 |a ^tVeterinary Immunology and Immunopathology^cVet. Immunol. Immunopathol. 
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900 |a 169 
900 |a ^i 
900 |a Vol. 146, no. 2 
900 |a 176 
900 |a BACTERIAL GHOSTS 
900 |a CATTLE 
900 |a E. COLI O157:H7 
900 |a VACCINE 
900 |a BACTERIAL VACCINE 
900 |a IMMUNOGLOBULIN A 
900 |a IMMUNOGLOBULIN G 
900 |a ANIMAL EXPERIMENT 
900 |a ANIMAL TISSUE 
900 |a BACTERIAL COLONIZATION 
900 |a BACTERIAL GHOST 
900 |a CONTROLLED STUDY 
900 |a CYTOPLASM 
900 |a ESCHERICHIA COLI 
900 |a HUMORAL IMMUNITY 
900 |a IMMUNIZATION 
900 |a IMMUNOGLOBULIN BLOOD LEVEL 
900 |a MALE 
900 |a NONHUMAN 
900 |a PROTEIN EXPRESSION 
900 |a SALIVA ANALYSIS 
900 |a VACCINATION 
900 |a WESTERN BLOTTING 
900 |a ANIMALS 
900 |a ANTIBODIES, BACTERIAL 
900 |a BACTERIAL SHEDDING 
900 |a CATTLE DISEASES 
900 |a ENZYME-LINKED IMMUNOSORBENT ASSAY 
900 |a ESCHERICHIA COLI INFECTIONS 
900 |a ESCHERICHIA COLI O157 
900 |a ESCHERICHIA COLI VACCINES 
900 |a RANDOM ALLOCATION 
900 |a ANIMALIA 
900 |a BACTERIA [MICROORGANISMS] 
900 |a BOS 
900 |a BOVINAE 
900 |a NEGIBACTERIA 
900 |a Cattle are the main reservoir of enterohemorrhagic . Escherichia coli O157:H7, a bacterium that, in humans, causes hemorrhagic colitis and hemolytic uremic syndrome [HUS], a life-threatening disease, especially in children and older people. Therefore, the development of vaccines preventing colonization of cattle by . E. coli O157:H7 could be a main tool for an HUS control program. In the present study, we evaluated bacterial ghosts [BGs] of . E. coli O157:H7 as an experimental vaccine against this pathogen. BGs are empty envelopes of Gram-negative bacteria, which retain the morphological surface make-up of their living counterparts and are produced by controlled expression of the cloned protein E, which causes loss of all the cytoplasm content. In this work, . E. coli O157:H7 BGs were used for subcutaneous immunization of calves. The vaccinated animals elicited significant levels of BG-specific IgG but not IgA antibodies in serum. Low levels of IgA and IgG antibodies against BGs were detected in saliva from vaccinated animals. Following oral challenge with . E. coli O157:H7, a significant reduction in both the duration and total bacterial shedding was observed in vaccinated calves compared to the nonimmunized group. We demonstrated that systemic vaccination with . E. coli O157 BGs provides protection in a bovine experimental model. Further research is needed to reach a higher mucosal immune response leading to an optimal vaccine. 
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