Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons

There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato [Solanum tuberosum L., cv. Spunta] stolons and to identify phosphory...

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Detalles Bibliográficos
Otros Autores: Cenzano, Ana Madrid, Cantoro, Renata, Hernández Sotomayor, S. M. Teresa, Abdala, Guillermina I., Racagni, Graciela E.
Formato: Artículo
Lenguaje:Inglés
Materias:
ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY
GALACTOLIPID
KINASE
PHOSPHOLIPID
POTATO
SOLANUM TUBEROSUM
BIOCHEMISTRY
ENZYMES
FATTY ACIDS
GLYCEROL
IONIZATION
MASS SPECTROMETRY
METABOLISM
PHOSPHOLIPIDS
PHOSPHORYLATION
ELECTROSPRAY IONIZATION
PHOSPHOTRANSFERASE
VEGETABLE PROTEIN
CHEMISTRY
ELECTROSPRAY MASS SPECTROMETRY
ENZYMOLOGY
EVALUATION
GROWTH, DEVELOPMENT AND AGING
LIPID METABOLISM
METHODOLOGY
PLANT TUBER
PHOSPHOTRANSFERASES
PLANT PROTEINS
PLANT TUBERS
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012Cenzano.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons 
520 |a There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato [Solanum tuberosum L., cv. Spunta] stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid [PA]; digalactosyldiacylglycerol [DGDG] was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine [PC], phosphatidylinositol [PI], and phosphatidylethanolamine [PE]. The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate [DGPP] by phosphatidic acid kinase [PAK]. This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. 
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773 |t Journal of Agricultural and Food Chemistry  |g Vol.60, no.1 (2012), p.418-426 
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