O - glycosylated cell wall proteins are essential in root hair growth

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Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins [HRGPs] that include extensins [EXTs] and arabinogalactan-proteins [AGPs]. Proline hydroxylation, an early...

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Detalles Bibliográficos
Otros Autores: Velasquez, Silvia M., Ricardi, Martiniano María, Gloazzo Dorosz, Javier, Fernández, Paula Virginia, Nadra, Alejandra D., Pol Fachin, Laercio, Egelund, Jack, Gille, Sasch, Harholt, Jespe, Ciancia, Marina, Verli, Hugo, Pauly, Markus, Bacic, Antony, Olsen, Carl Erik, Ulvskov, Peter, Petersen, Bent Larsen, Somerville, Chris, Iusem, Norberto D., Estévez, José Manuel
Formato: Artículo
Lenguaje:Inglés
Materias:
ARABINOGALACTAN
ARABINOSYLTRANSFERASE
CELL MEMBRANE PROTEIN
EXTENSIN
GLYCAN
GLYCOPROTEIN
GREEN FLUORESCENT PROTEIN
HYDROXYPROLINE RICH GLYCOPROTEIN
PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE
PROLINE RICH PROTEIN
TRANSFERASE
UNCLASSIFIED DRUG
ADSORPTION
DICOTYLEDON
ENZYME ACTIVITY
HAIR
INHIBITION
POLYSACCHARIDE
PROTEIN
ARABIDOPSIS
ARABINOSYLATION
CELL GROWTH
CELL WALL
CHEMICAL ANALYSIS
CHEMICAL REACTION
GLYCOSYLATION
HYDROXYLATION
NONHUMAN
PROTEIN EXPRESSION
PROTEIN FUNCTION
ROOT HAIR
ARABIDOPSIS PROTEINS
ARABINOSE
CARBOHYDRATE CONFORMATION
GENE EXPRESSION REGULATION, PLANT
GENES, PLANT
GLYCOPROTEINS
GLYCOSYLTRANSFERASES
HYDROXYPROLINE
MODELS, BIOLOGICAL
MUTATION
PENTOSYLTRANSFERASES
PHENOTYPE
PLANT PROTEINS
PLANT ROOTS
POLYSACCHARIDES
PROCOLLAGEN-PROLINE DIOXYGENASE
PROLINE
PROTEIN CONFORMATION
PROTEIN PROCESSING, POST-TRANSLATIONAL
PROTEIN STRUCTURE, SECONDARY
ARABIDOPSIS THALIANA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2011Velasquez.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a O - glycosylated cell wall proteins are essential in root hair growth 
520 |a Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins [HRGPs] that include extensins [EXTs] and arabinogalactan-proteins [AGPs]. Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases [P4Hs], defines the subsequent O-glycosylation sites in EXTs [which are mainly arabinosylated] and AGPs [which are mainly arabinogalactosylated]. We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana. 
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653 0 |a GLYCAN 
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653 0 |a PROTEIN FUNCTION 
653 0 |a ROOT HAIR 
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653 0 |a ARABINOSE 
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653 0 |a PROTEIN STRUCTURE, SECONDARY 
653 0 |a ARABIDOPSIS THALIANA 
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700 1 |9 67546  |a Ricardi, Martiniano María 
700 1 |9 69900  |a Gloazzo Dorosz, Javier 
700 1 |9 67318  |a Fernández, Paula Virginia 
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700 1 |a Gille, Sasch  |9 69904 
700 1 |a Harholt, Jespe  |9 69905 
700 1 |9 46099  |a Ciancia, Marina 
700 1 |a Verli, Hugo  |9 69906 
700 1 |a Pauly, Markus   |9 69907 
700 1 |a Bacic, Antony  |9 69908 
700 1 |a Olsen, Carl Erik  |9 69909 
700 1 |a Ulvskov, Peter  |9 69910 
700 1 |a Petersen, Bent Larsen  |9 69911 
700 1 |a Somerville, Chris  |9 69912 
700 1 |a Iusem, Norberto D.   |9 69913 
700 1 |a Estévez, José Manuel  |9 67772 
773 |t Science  |g Vol.332, no.6036 (2011), p.1401-1403 
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900 |a ^aIusem^bN. D. 
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900 |a ^aVelasquez^bS.M.^tInst. de Fisiol., Biologia Molec. Y Neurociencias-Consejo Nac. de Invest. Cientificas Y Tec., Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina 
900 |a ^aRicardi^bM.M.^tDepto. de Biologia Aplicada Y Alimentos [Centro de Investigaciones en Hidratos de Carbono-CONICET], Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires CD1417DSE, Argentina 
900 |a ^aDorosz^bJ.G.^tDepartamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina 
900 |a ^aFernandez^bP.V.^tPrograma de Pos-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, 91500-979, United Kingdom 
900 |a ^aNadra^bA.D.^tFaculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Brazil 
900 |a ^aPol-Fachin^bL.^tDepartment of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Copenhagen 1871, Denmark 
900 |a ^aEgelund^bJ.^tEnergy Biosciences Institute, University of California, Berkeley, Berkeley, CA 94720, United States 
900 |a ^aGille^bS.^tPlant Cell Biology Research Centre, School of Botany, University of Melbourne, Melbourne, VIC 3010, Australia 
900 |a ^aHarholt^bJ.^tDepartment of Natural Sciences, Faculty of Life Sciences, University of Copenhagen, 1871, Denmark 
900 |a ^aCiancia^bM.^tDepartamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina 
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900 |a PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE 
900 |a PROLINE RICH PROTEIN 
900 |a TRANSFERASE 
900 |a UNCLASSIFIED DRUG 
900 |a ADSORPTION 
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900 |a INHIBITION 
900 |a POLYSACCHARIDE 
900 |a PROTEIN 
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900 |a CELL GROWTH 
900 |a CELL WALL 
900 |a CHEMICAL ANALYSIS 
900 |a CHEMICAL REACTION 
900 |a GLYCOSYLATION 
900 |a HYDROXYLATION 
900 |a NONHUMAN 
900 |a PROTEIN EXPRESSION 
900 |a PROTEIN FUNCTION 
900 |a ROOT HAIR 
900 |a ARABIDOPSIS PROTEINS 
900 |a ARABINOSE 
900 |a CARBOHYDRATE CONFORMATION 
900 |a GENE EXPRESSION REGULATION, PLANT 
900 |a GENES, PLANT 
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900 |a PROTEIN STRUCTURE, SECONDARY 
900 |a ARABIDOPSIS THALIANA 
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