Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

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The aim of this study was to identify the in vitro development stage at which the culture of a single or low number [n = 5 or 10] of oocytes/embryos could impair development in comparison with culture in group [n = 50]. In the Experiment 1, it was confirmed that single in vitro embryo production yie...

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Detalles Bibliográficos
Otros Autores: Salvador, I., Cebrian Serrano, A., Salamone, Daniel Felipe, Silvestre, M. A.
Formato: Artículo
Lenguaje:Inglés
Materias:
LOW EMBRYO CULTURE
SINGLE
BOVINAE
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2011Salvador.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine 
246 0 |a Efecto del número de ovocitos y embriones en la maduración, fertilización y desarrollo embrionario in vitro en vacuno 
520 |a The aim of this study was to identify the in vitro development stage at which the culture of a single or low number [n = 5 or 10] of oocytes/embryos could impair development in comparison with culture in group [n = 50]. In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group [49.4 vs. 83.0 percent; 0 percent vs. 37.8 percent, respectively; p less than 0.05]. In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture [2.9; 10.2-10.8; 33.2 percent in single, low number of embryos [5-10], and controlgrouped, respectively; p less than 0.05]. In Experiment 5, it was observed that the last part of the culture period [day 3 onwards] seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number [5-10] of embryos culture. 
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773 |t Spanish Journal of Agricultural Research  |g Vol.9, no.3 (2011), p.744-752 
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900 |a ^aSalvador^bI.^tCentro de Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias [CITA-IVIA], Apdo. 187, 12400 Segorbe [Castellón], Spain 
900 |a ^aCebrian-Serrano^bA.^tLaboratorio de Biotecnología Animal, Facultad de Agronomía, Universidad de Buenos Aires, Argentina 
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900 |a The aim of this study was to identify the in vitro development stage at which the culture of a single or low number [n = 5 or 10] of oocytes/embryos could impair development in comparison with culture in group [n = 50]. In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group [49.4 vs. 83.0 percent; 0 percent vs. 37.8 percent, respectively; p less than 0.05]. In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture [2.9; 10.2-10.8; 33.2 percent in single, low number of embryos [5-10], and controlgrouped, respectively; p less than 0.05]. In Experiment 5, it was observed that the last part of the culture period [day 3 onwards] seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number [5-10] of embryos culture. 
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