Glyphosate resistance in perennial Sorghum halepense [Johnsongrass], endowed by reduced glyphosate translocation and leaf uptake
Background: In a large cropping area of northern Argentina, Sorghum halepense [Johnsongrass] has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSP...
| Otros Autores: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2012VilaAiub.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
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| 024 | |a 10.1002/ps.2286 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | 0 | |a Glyphosate resistance in perennial Sorghum halepense [Johnsongrass], endowed by reduced glyphosate translocation and leaf uptake |
| 520 | |a Background: In a large cropping area of northern Argentina, Sorghum halepense [Johnsongrass] has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. Results: Individuals of all resistant [R] accessions exhibited significantly less glyphosate translocation to root [11 percent versus 29 percent] and stem [9 percent versus 26 percent] meristems when compared with susceptible [S] plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants [63 percent] than in the treated leaves of S plants [27 percent]. In addition, individuals of S. halepense accession R 2 consistently showed lower glyphosate absorption rates in both adaxial [10-20 percent] and abaxial [20-25 percent] leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101-106 residues were found in any of the evaluated R accessions. Conclusion: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. | ||
| 653 | 0 | |a GLYPHOSATE LEAF UPTAKE | |
| 653 | 0 | |a GLYPHOSATE TRANSLOCATION | |
| 653 | 0 | |a NON-TARGET-SITE RESISTANCE MECHANISM | |
| 653 | 0 | |a PERENNIAL JOHNSONGRASS WEED | |
| 653 | 0 | |a DRUG DERIVATIVE | |
| 653 | 0 | |a GLYCINE | |
| 653 | 0 | |a GLYPHOSATE | |
| 653 | 0 | |a HERBICIDE | |
| 653 | 0 | |a VEGETABLE PROTEIN | |
| 653 | 0 | |a ASSESSMENT METHOD | |
| 653 | 0 | |a BIOCHEMICAL COMPOSITION | |
| 653 | 0 | |a BIOLOGICAL UPTAKE | |
| 653 | 0 | |a CARBON ISOTOPE | |
| 653 | 0 | |a MOLECULAR ANALYSIS | |
| 653 | 0 | |a PESTICIDE RESISTANCE | |
| 653 | 0 | |a SORGHUM | |
| 653 | 0 | |a TRANSLOCATION | |
| 653 | 0 | |a DRUG EFFECT | |
| 653 | 0 | |a GENETICS | |
| 653 | 0 | |a HERBICIDE RESISTANCE | |
| 653 | 0 | |a METABOLISM | |
| 653 | 0 | |a PLANT LEAF | |
| 653 | 0 | |a TRANSPORT AT THE CELLULAR LEVEL | |
| 653 | 0 | |a BIOLOGICAL TRANSPORT | |
| 653 | 0 | |a HERBICIDE RESISTANCE | |
| 653 | 0 | |a PLANT LEAVES | |
| 653 | 0 | |a PLANT PROTEINS | |
| 653 | 0 | |a ARGENTINA | |
| 653 | 0 | |a SORGHUM HALEPENSE | |
| 653 | 0 | |a SORGHUM X ALMUM | |
| 700 | 1 | |9 9201 |a Vila Aiub, Martín Miguel | |
| 700 | 1 | |a Balbi, María Celeste |9 12411 | |
| 700 | 1 | |a Distéfano, Ana J. |9 69516 | |
| 700 | 1 | |a Fernández, Luis |9 69517 | |
| 700 | 1 | |9 41118 |a Hopp, Horacio Esteban | |
| 700 | 1 | |9 67249 |a Yu, Qin | |
| 700 | 1 | |9 67250 |a Powles, Stephen B. | |
| 773 | |t Pest Management Science |g Vol.68, no.3 (2012), p.430-436 | ||
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| 900 | |a ^tGlyphosate resistance in perennial Sorghum halepense [Johnsongrass], endowed by reduced glyphosate translocation and leaf uptake | ||
| 900 | |a ^aVila-Aiub^bM.M. | ||
| 900 | |a ^aBalbi^bM.C. | ||
| 900 | |a ^aDistéfano^bA.J. | ||
| 900 | |a ^aFernández^bL. | ||
| 900 | |a ^aHopp^bE. | ||
| 900 | |a ^aYu^bQ. | ||
| 900 | |a ^aPowles^bS.B. | ||
| 900 | |a ^aVila Aiub^bM. M. | ||
| 900 | |a ^aBalbi^bM. C. | ||
| 900 | |a ^aDistéfano^bA. J. | ||
| 900 | |a ^aFernández^bL. | ||
| 900 | |a ^aHopp^bH. E. | ||
| 900 | |a ^aYu^bQ. | ||
| 900 | |a ^aPowles^bS. B. | ||
| 900 | |a ^aVila-Aiub^bM.M.^tIFEVA-CONICET, Facultad de AgronomÃa, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 900 | |a ^aBalbi^bM.C.^tMonsanto Argentina, Buenos Aires, Argentina | ||
| 900 | |a ^aDistéfano^bA.J.^tInstituto de BiotecnologÃa, CICVyA, Instituto Nacional de TecnologÃa Agropecuaria [INTA Castelar], Hurlingham, Argentina | ||
| 900 | |a ^aFernández^bL.^tFacultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 900 | |a ^aHopp^bE.^tAHRI-School of Plant Biology, Institute of Agriculture, University of Western Australia, Australia | ||
| 900 | |a ^aYu^bQ. | ||
| 900 | |a ^aPowles^bS.B. | ||
| 900 | |a ^tPest Management Science^cPest Manage. Sci. | ||
| 900 | |a en | ||
| 900 | |a 430 | ||
| 900 | |a ^i | ||
| 900 | |a Vol. 68, no. 3 | ||
| 900 | |a 436 | ||
| 900 | |a GLYPHOSATE LEAF UPTAKE | ||
| 900 | |a GLYPHOSATE TRANSLOCATION | ||
| 900 | |a NON-TARGET-SITE RESISTANCE MECHANISM | ||
| 900 | |a PERENNIAL JOHNSONGRASS WEED | ||
| 900 | |a DRUG DERIVATIVE | ||
| 900 | |a GLYCINE | ||
| 900 | |a GLYPHOSATE | ||
| 900 | |a HERBICIDE | ||
| 900 | |a VEGETABLE PROTEIN | ||
| 900 | |a ASSESSMENT METHOD | ||
| 900 | |a BIOCHEMICAL COMPOSITION | ||
| 900 | |a BIOLOGICAL UPTAKE | ||
| 900 | |a CARBON ISOTOPE | ||
| 900 | |a MOLECULAR ANALYSIS | ||
| 900 | |a PESTICIDE RESISTANCE | ||
| 900 | |a SORGHUM | ||
| 900 | |a TRANSLOCATION | ||
| 900 | |a DRUG EFFECT | ||
| 900 | |a GENETICS | ||
| 900 | |a HERBICIDE RESISTANCE | ||
| 900 | |a METABOLISM | ||
| 900 | |a PLANT LEAF | ||
| 900 | |a TRANSPORT AT THE CELLULAR LEVEL | ||
| 900 | |a BIOLOGICAL TRANSPORT | ||
| 900 | |a HERBICIDE RESISTANCE | ||
| 900 | |a PLANT LEAVES | ||
| 900 | |a PLANT PROTEINS | ||
| 900 | |a ARGENTINA | ||
| 900 | |a SORGHUM HALEPENSE | ||
| 900 | |a SORGHUM X ALMUM | ||
| 900 | |a Background: In a large cropping area of northern Argentina, Sorghum halepense [Johnsongrass] has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. Results: Individuals of all resistant [R] accessions exhibited significantly less glyphosate translocation to root [11 percent versus 29 percent] and stem [9 percent versus 26 percent] meristems when compared with susceptible [S] plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants [63 percent] than in the treated leaves of S plants [27 percent]. In addition, individuals of S. halepense accession R 2 consistently showed lower glyphosate absorption rates in both adaxial [10-20 percent] and abaxial [20-25 percent] leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101-106 residues were found in any of the evaluated R accessions. Conclusion: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. | ||
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