Potato Snakin - 1 gene silencing affects cell division, primary metabolism, and cell wall composition

Mostrar otras versiones (2)

Snakin-1 [SN1] is an antimicrobial cysteine-rich peptide isolated from potato [Solanum tuberosum] that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overex...

Descripción completa

Guardado en:
Detalles Bibliográficos
Otros Autores: Nahirñak, Vanesa, Almasia, Natalia Inés, Fernández, Paula Virginia, Hopp, Horacio Esteban, Carrari, Fernando, Vazquez Rovere, Cecilia
Formato: Artículo
Lenguaje:Español
Materias:
GREEN FLUORESCENT PROTEIN
SN1 PROTEIN, SOLANUM TUBEROSUM
VEGETABLE PROTEIN
CELL DIVISION
CELL MEMBRANE
CELL WALL
CHEMISTRY
CYTOLOGY
GENE EXPRESSION REGULATION
GENE SILENCING
GENETICS
INFRARED SPECTROSCOPY
MASS FRAGMENTOGRAPHY
METABOLISM
MOLECULAR GENETICS
NUCLEOTIDE SEQUENCE
PHYSIOLOGY
PLANT EPIDERMIS
PLANT LEAF
POTATO
SOLANACEAE
TRANSGENIC PLANT
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
MOLECULAR SEQUENCE DATA
PLANT LEAVES
PLANT PROTEINS
PLANTS, GENETICALLY MODIFIED
SOLANUM TUBEROSUM
SPECTROSCOPY, FOURIER TRANSFORM INFRARED
ARABIDOPSIS
NICOTIANA BENTHAMIANA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012Nahirnak.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
LEADER 09012cab a22016697a 4500
001 AR-BaUFA000102
003 AR-BaUFA
005 20220418145042.0
008 181208t2012 |||||o|||||00||||spa d
999 |c 46536  |d 46536 
022 |a 0032-0889 
024 |a 10.1104/pp.111.186544 
040 |a AR-BaUFA  |c AR-BaUFA 
245 1 0 |a Potato Snakin - 1 gene silencing affects cell division, primary metabolism, and cell wall composition 
520 |a Snakin-1 [SN1] is an antimicrobial cysteine-rich peptide isolated from potato [Solanum tuberosum] that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70 percent to 90 percent increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. 
653 0 |a GREEN FLUORESCENT PROTEIN 
653 0 |a SN1 PROTEIN, SOLANUM TUBEROSUM 
653 0 |a VEGETABLE PROTEIN 
653 0 |a CELL DIVISION 
653 0 |a CELL MEMBRANE 
653 0 |a CELL WALL 
653 0 |a CHEMISTRY 
653 0 |a CYTOLOGY 
653 0 |a GENE EXPRESSION REGULATION 
653 0 |a GENE SILENCING 
653 0 |a GENETICS 
653 0 |a INFRARED SPECTROSCOPY 
653 0 |a MASS FRAGMENTOGRAPHY 
653 0 |a METABOLISM 
653 0 |a MOLECULAR GENETICS 
653 0 |a NUCLEOTIDE SEQUENCE 
653 0 |a PHYSIOLOGY 
653 0 |a PLANT EPIDERMIS 
653 0 |a PLANT LEAF 
653 0 |a POTATO 
653 0 |a SOLANACEAE 
653 0 |a TRANSGENIC PLANT 
653 0 |a GAS CHROMATOGRAPHY-MASS SPECTROMETRY 
653 0 |a MOLECULAR SEQUENCE DATA 
653 0 |a PLANT LEAVES 
653 0 |a PLANT PROTEINS 
653 0 |a PLANTS, GENETICALLY MODIFIED 
653 0 |a SOLANUM TUBEROSUM 
653 0 |a SPECTROSCOPY, FOURIER TRANSFORM INFRARED 
653 0 |a ARABIDOPSIS 
653 0 |a NICOTIANA BENTHAMIANA 
700 1 |a Nahirñak, Vanesa  |9 37391 
700 1 |9 69514  |a Almasia, Natalia Inés 
700 1 |9 67318  |a Fernández, Paula Virginia 
700 1 |9 41118  |a Hopp, Horacio Esteban 
700 1 |9 11158  |a Carrari, Fernando 
700 1 |a Vazquez Rovere, Cecilia   |9 58212 
773 |t Plant Physiology  |g Vol.158, no.1 (2012), p.252-263 
856 |u http://ri.agro.uba.ar/files/intranet/articulo/2012Nahirnak.pdf  |i En reservorio  |q application/pdf  |f 2012Nahirnak  |x MIGRADOS2018 
856 |u http://my.aspb.org/  |x MIGRADOS2018  |z LINK AL EDITOR 
900 |a as 
900 |a 20131220 
900 |a N 
900 |a SCOPUS 
900 |a EMBARGO 
900 |a a 
900 |a s 
900 |a ARTICULO 
900 |a EN LINEA 
900 |a 00320889 
900 |a 10.1104/pp.111.186544 
900 |a ^tPotato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition 
900 |a ^aNahirñak^bV. 
900 |a ^aAlmasia^bN.I. 
900 |a ^aFernández^bP.V. 
900 |a ^aHopp^bH.E. 
900 |a ^aEstevez^bJ.M. 
900 |a ^aCarrari^bF. 
900 |a ^aVazquez-Rovere^bC. 
900 |a ^aNahirñak^bV. 
900 |a ^aAlmasia^bN. I. 
900 |a ^aFernández^bP. V. 
900 |a ^aHopp^bH. E. 
900 |a ^aEstevez^bJ. M. 
900 |a ^aCarrari^bF. 
900 |a ^aVazquez Rovere^bC. 
900 |a ^aNahirñak^bV.^tInstituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnologia Agropecuaria, Repetto y De Los Reseros s/n, CP 1686, Hurlingham, Buenos Aires, Argentina 
900 |a ^aAlmasia^bN.I.^tConsejo Nacional de Investigaciones Científicas y Técnicas, C1033AAJ Buenos Aires, Argentina 
900 |a ^aFernandez^bP.V.^tCátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, CP C1417DSE, Buenos Aires, Argentina 
900 |a ^aHopp^bH.E.^tInstituto de Fisiología, Biología Molecular y Neurociencias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, CP 1428, Buenos Aires, Argentina 
900 |a ^aEstevez^bJ.M. 
900 |a ^aCarrari^bF. 
900 |a ^aVazquez-Rovere^bC. 
900 |a ^tPlant Physiology^cPlant Physiol. 
900 |a eng 
900 |a 252 
900 |a ^i 
900 |a Vol. 158, no. 1 
900 |a 263 
900 |a GREEN FLUORESCENT PROTEIN 
900 |a SN1 PROTEIN, SOLANUM TUBEROSUM 
900 |a VEGETABLE PROTEIN 
900 |a CELL DIVISION 
900 |a CELL MEMBRANE 
900 |a CELL WALL 
900 |a CHEMISTRY 
900 |a CYTOLOGY 
900 |a GENE EXPRESSION REGULATION 
900 |a GENE SILENCING 
900 |a GENETICS 
900 |a INFRARED SPECTROSCOPY 
900 |a MASS FRAGMENTOGRAPHY 
900 |a METABOLISM 
900 |a MOLECULAR GENETICS 
900 |a NUCLEOTIDE SEQUENCE 
900 |a PHYSIOLOGY 
900 |a PLANT EPIDERMIS 
900 |a PLANT LEAF 
900 |a POTATO 
900 |a SOLANACEAE 
900 |a TRANSGENIC PLANT 
900 |a GAS CHROMATOGRAPHY-MASS SPECTROMETRY 
900 |a MOLECULAR SEQUENCE DATA 
900 |a PLANT LEAVES 
900 |a PLANT PROTEINS 
900 |a PLANTS, GENETICALLY MODIFIED 
900 |a SOLANUM TUBEROSUM 
900 |a SPECTROSCOPY, FOURIER TRANSFORM INFRARED 
900 |a ARABIDOPSIS 
900 |a NICOTIANA BENTHAMIANA 
900 |a Snakin-1 [SN1] is an antimicrobial cysteine-rich peptide isolated from potato [Solanum tuberosum] that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70 percent to 90 percent increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. 
900 |a 158 
900 |a 1 
900 |a 2012 
900 |a ^cH 
900 |a AAG 
900 |a AGROVOC 
900 |a 2012Nahirnak 
900 |a AAG 
900 |a http://ri.agro.uba.ar/files/intranet/articulo/2012Nahirnak.pdf 
900 |a 2012Nahirnak.pdf 
900 |a http://my.aspb.org/ 
900 |a http://www.scopus.com/inward/record.url?eid=2-s2.0-84855265971&partnerID=40&md5=21458cfcf08b9d8cbc3e5cd017e1f111 
900 |a ^a^b^c^d^e^f^g^h^i 
900 |a OS 
942 0 0 |c ARTICULO  |2 udc 
942 0 0 |c ENLINEA  |2 udc 

Ejemplares similares