Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose

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Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in...

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Detalles Bibliográficos
Otros Autores: Ramos, Araceli M., Gally, Marcela Edith, García, María Celia, Levin, Laura
Formato: Artículo
Lenguaje:Inglés
Materias:
ANTHRACNOSE
COLLETOTRICHUM TRUNCATUM
PECTINOLYTIC ENZYMES
SOYBEAN
PECTIN
PECTIN LYASE
POLYGALACTURONASE
COLLETOTRICHUM
CONTROLLED STUDY
CULTURE MEDIUM
ENZYME ACTIVITY
ENZYME SYNTHESIS
FUNGAL STRAIN
FUNGAL VIRULENCE
FUNGUS CULTURE
FUNGUS GROWTH
FUNGUS ISOLATION
GEOGRAPHIC DISTRIBUTION
IN VITRO STUDY
NONHUMAN
PH MEASUREMENT
PLANT PATHOGEN INTERACTION
PECTINS
PLANT DISEASES
SOYBEANS
FUNGI
GLYCINE MAX
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2010Ramos.pdf
LINK AL EDITOR.
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
Descripción
Sumario:Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographic locations, as a preliminary step to establish the biological role of the pectinolytic enzymes in the Colletotrichum spp.-soybean system, yet unknown. Methods: Ten strains were screened for in vitro pectinolytic enzyme production on a defined medium based on pectin as carbon source. Results: All isolates were able to grow in this medium and polymethylgalacturonase [PMG], polygalacturonase [PG] and pectin lyase [PL] activities were detected. On the whole, the peak of polygalacturonases activities preceded the day of maximum growth, while PL activity reached its highest level afterwards. Strain BAFC 3097 [from Santa Fe province] yielded high titles of the three enzymes [1.08. U/ml PG, 1.05. U/ml PMG, 156. U/ml PL], after a short incubation period [7-10 days]. Low synthesis of polygalacturonases in cultures containing glucose as unique carbon source suggests that these enzymes are constitutive in contrast with PL, which was not detected. Conclusions: The disparity observed in enzyme production among strains cannot be related to fungal growth, since no major differences in mycelial yield were found; it was not connected with their geographic origin, but might be associated with differences in virulence among strains not yet evaluated.
ISSN:1130-1406

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