Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose
Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2010Ramos.pdf LINK AL EDITOR. |
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| 245 | 1 | 0 | |a Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose |
| 246 | 1 | 0 | |a Producción de enzimas pectinolíticas por Colletotrichum truncatum, agente causal de antracnosis en soja |
| 520 | |a Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographic locations, as a preliminary step to establish the biological role of the pectinolytic enzymes in the Colletotrichum spp.-soybean system, yet unknown. Methods: Ten strains were screened for in vitro pectinolytic enzyme production on a defined medium based on pectin as carbon source. Results: All isolates were able to grow in this medium and polymethylgalacturonase [PMG], polygalacturonase [PG] and pectin lyase [PL] activities were detected. On the whole, the peak of polygalacturonases activities preceded the day of maximum growth, while PL activity reached its highest level afterwards. Strain BAFC 3097 [from Santa Fe province] yielded high titles of the three enzymes [1.08. U/ml PG, 1.05. U/ml PMG, 156. U/ml PL], after a short incubation period [7-10 days]. Low synthesis of polygalacturonases in cultures containing glucose as unique carbon source suggests that these enzymes are constitutive in contrast with PL, which was not detected. Conclusions: The disparity observed in enzyme production among strains cannot be related to fungal growth, since no major differences in mycelial yield were found; it was not connected with their geographic origin, but might be associated with differences in virulence among strains not yet evaluated. | ||
| 653 | 0 | |a ANTHRACNOSE | |
| 653 | 0 | |a COLLETOTRICHUM TRUNCATUM | |
| 653 | 0 | |a PECTINOLYTIC ENZYMES | |
| 653 | 0 | |a SOYBEAN | |
| 653 | 0 | |a PECTIN | |
| 653 | 0 | |a PECTIN LYASE | |
| 653 | 0 | |a POLYGALACTURONASE | |
| 653 | 0 | |a COLLETOTRICHUM | |
| 653 | 0 | |a CONTROLLED STUDY | |
| 653 | 0 | |a CULTURE MEDIUM | |
| 653 | 0 | |a ENZYME ACTIVITY | |
| 653 | 0 | |a ENZYME SYNTHESIS | |
| 653 | 0 | |a FUNGAL STRAIN | |
| 653 | 0 | |a FUNGAL VIRULENCE | |
| 653 | 0 | |a FUNGUS CULTURE | |
| 653 | 0 | |a FUNGUS GROWTH | |
| 653 | 0 | |a FUNGUS ISOLATION | |
| 653 | 0 | |a GEOGRAPHIC DISTRIBUTION | |
| 653 | 0 | |a IN VITRO STUDY | |
| 653 | 0 | |a NONHUMAN | |
| 653 | 0 | |a PH MEASUREMENT | |
| 653 | 0 | |a PLANT PATHOGEN INTERACTION | |
| 653 | 0 | |a COLLETOTRICHUM | |
| 653 | 0 | |a PECTINS | |
| 653 | 0 | |a PLANT DISEASES | |
| 653 | 0 | |a SOYBEANS | |
| 653 | 0 | |a FUNGI | |
| 653 | 0 | |a GLYCINE MAX | |
| 700 | 1 | |9 67629 |a Ramos, Araceli M. | |
| 700 | 1 | |9 15444 |a Gally, Marcela Edith | |
| 700 | |a García, María Celia |9 26202 | ||
| 700 | 1 | |a Levin, Laura |9 67794 | |
| 773 | |t Revista Iberoamericana de Micología |g Vol.27, no.4 (2010), p.186-190 | ||
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| 900 | |a ^tPectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose | ||
| 900 | |a ^tProducción de enzimas pectinolíticas por Colletotrichum truncatum, agente causal de antracnosis en soja | ||
| 900 | |a ^aRamos^bA.M. | ||
| 900 | |a ^aGally^bM. | ||
| 900 | |a ^aGarcía^bM.C. | ||
| 900 | |a ^aLevin^bL. | ||
| 900 | |a ^aRamos^bA. M. | ||
| 900 | |a ^aGally^bM. E. | ||
| 900 | |a ^aGarcía^bM. C. | ||
| 900 | |a ^aLevin^bL. | ||
| 900 | |a ^aRamos, A.M.^tDpto. de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, PROPLAME-PRHIDEB-CONICET, Universidad de Buenos Aires, Argentina | ||
| 900 | |a ^aGally, M.^tCátedra de Fitopatología, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina | ||
| 900 | |a ^aGarcía, M.C.^t | ||
| 900 | |a ^aLevin, L.^t | ||
| 900 | |a ^tRevista Iberoamericana de Micología^cRev. Iberoam. Micol. | ||
| 900 | |a en | ||
| 900 | |a 186 | ||
| 900 | |a ^i | ||
| 900 | |a Vol. 27, no. 4 | ||
| 900 | |a 190 | ||
| 900 | |a ANTHRACNOSE | ||
| 900 | |a COLLETOTRICHUM TRUNCATUM | ||
| 900 | |a PECTINOLYTIC ENZYMES | ||
| 900 | |a SOYBEAN | ||
| 900 | |a PECTIN | ||
| 900 | |a PECTIN LYASE | ||
| 900 | |a POLYGALACTURONASE | ||
| 900 | |a COLLETOTRICHUM | ||
| 900 | |a CONTROLLED STUDY | ||
| 900 | |a CULTURE MEDIUM | ||
| 900 | |a ENZYME ACTIVITY | ||
| 900 | |a ENZYME SYNTHESIS | ||
| 900 | |a FUNGAL STRAIN | ||
| 900 | |a FUNGAL VIRULENCE | ||
| 900 | |a FUNGUS CULTURE | ||
| 900 | |a FUNGUS GROWTH | ||
| 900 | |a FUNGUS ISOLATION | ||
| 900 | |a GEOGRAPHIC DISTRIBUTION | ||
| 900 | |a IN VITRO STUDY | ||
| 900 | |a NONHUMAN | ||
| 900 | |a PH MEASUREMENT | ||
| 900 | |a PLANT PATHOGEN INTERACTION | ||
| 900 | |a COLLETOTRICHUM | ||
| 900 | |a PECTINS | ||
| 900 | |a PLANT DISEASES | ||
| 900 | |a SOYBEANS | ||
| 900 | |a FUNGI | ||
| 900 | |a GLYCINE MAX | ||
| 900 | |a Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographic locations, as a preliminary step to establish the biological role of the pectinolytic enzymes in the Colletotrichum spp.-soybean system, yet unknown. Methods: Ten strains were screened for in vitro pectinolytic enzyme production on a defined medium based on pectin as carbon source. Results: All isolates were able to grow in this medium and polymethylgalacturonase [PMG], polygalacturonase [PG] and pectin lyase [PL] activities were detected. On the whole, the peak of polygalacturonases activities preceded the day of maximum growth, while PL activity reached its highest level afterwards. Strain BAFC 3097 [from Santa Fe province] yielded high titles of the three enzymes [1.08. U/ml PG, 1.05. U/ml PMG, 156. U/ml PL], after a short incubation period [7-10 days]. Low synthesis of polygalacturonases in cultures containing glucose as unique carbon source suggests that these enzymes are constitutive in contrast with PL, which was not detected. Conclusions: The disparity observed in enzyme production among strains cannot be related to fungal growth, since no major differences in mycelial yield were found; it was not connected with their geographic origin, but might be associated with differences in virulence among strains not yet evaluated. | ||
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