Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type

HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in...

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Detalles Bibliográficos
Otros Autores: González, Fernanda Gabriela, Capella, Matías, Ribichich, Karina Fabiana, Curín, Facundo, Giacomelli, Jorge Ignacio, Ayala, Francisco, Watson, Gerónimo, Otegui, María Elena, Chan, Raquel Lía
Formato: Artículo
Lenguaje:Inglés
Materias:
DROUGHT TOLERANCE
GRAIN YIELD DETERMINATION
HAHB4
SUNFLOWER TRANSCRIPTION FACTOR
TRANSGENIC WHEAT
WATER USE EFFICIENCY
WHEAT FIELD TRIALS
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2019gonzalez1.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Field - grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type 
520 |a HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P smaller than 0.001) and had 9.4% larger water use efficiency (P smaller than 0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P smaller than 0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts. 
653 |a DROUGHT TOLERANCE 
653 |a GRAIN YIELD DETERMINATION 
653 |a HAHB4 
653 |a SUNFLOWER TRANSCRIPTION FACTOR 
653 |a TRANSGENIC WHEAT 
653 |a WATER USE EFFICIENCY 
653 |a WHEAT FIELD TRIALS 
700 1 |9 11379  |a González, Fernanda Gabriela  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino (EEA Pergamino). Buenos Aires, Argentina.  |u Universidad Nacional del Noroeste de la provincia de Buenos Aires (UNNOBA). CITNOBA. Pergamino, Buenos Aires, Argentina.  |u CONICET - Universidad Nacional del Noroeste de la provincia de Buenos Aires (UNNOBA). CITNOBA. Pergamino, Buenos Aires, Argentina. 
700 1 |a Capella, Matías  |u Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |u CONICET - Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |9 68738 
700 1 |9 68739  |a Ribichich, Karina Fabiana  |u Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |u CONICET - Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. 
700 1 |9 68740  |a Curín, Facundo  |u Universidad Nacional del Noroeste de la provincia de Buenos Aires (UNNOBA). CITNOBA. Pergamino, Buenos Aires, Argentina.  |u CONICET - Universidad Nacional del Noroeste de la provincia de Buenos Aires (UNNOBA). CITNOBA. Pergamino, Buenos Aires, Argentina. 
700 1 |a Giacomelli, Jorge Ignacio  |u Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |u CONICET - Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |9 68741 
700 1 |a Ayala, Francisco  |u INDEAR - BIOCERES. Rosario, Argentina.  |9 68742 
700 1 |9 68743  |a Watson, Gerónimo  |u INDEAR - BIOCERES. Rosario, Argentina. 
700 1 |9 5930  |a Otegui, María Elena  |u Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.  |u CONICET. Buenos Aires, Argentina.  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Buenos Aires, Argentina. 
700 1 |9 68770  |a Chan, Raquel Lía  |u Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina.  |u CONICET - Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral. Santa Fe, Argentina. 
773 0 |t Journal of experimental botany  |w SECS000114  |g vol.70, no.5 (2019), p.1669–1681, grafs., tbls., il., mapas, fot. 
856 |f 2019gonzalez1  |i en internet  |q application/pdf  |u http://ri.agro.uba.ar/files/download/articulo/2019gonzalez1.pdf  |x ARTI201904 
856 |z LINK AL EDITOR  |u http://global.oup.com 
942 |c ARTICULO 
942 |c ENLINEA 
976 |a AAG 

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