Shiga toxin - producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective:...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2017martorelli.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04080nab a22004337a 4500 | ||
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| 001 | 20180601092240.0 | ||
| 003 | AR-BaUFA | ||
| 005 | 20220318104306.0 | ||
| 008 | 180601t2017 ne |||||o|||| 00| | eng d | ||
| 999 | |c 45555 |d 45555 | ||
| 999 | |d 45555 | ||
| 999 | |d 45555 | ||
| 999 | |d 45555 | ||
| 999 | |d 45555 | ||
| 022 | |a 0378-1135 | ||
| 024 | |a 10.1016/j.vetmic.2017.06.021 | ||
| 040 | |a AR-BaUFA | ||
| 245 | 1 | 0 | |a Shiga toxin - producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo |
| 520 | |a Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection. In order to assess the basis of the interference, three STEC strains were isolated from STEC persistently-colonized healthy cattle from a dairy farm in Buenos Aires, Argentina. Methods and results: The three isolated strains are E. coli O22:H8 and carry the stx1 and stx2d genes. The activatable activity of Stx2d was demonstrated in vitro. The three strains carry the adhesins iha, ehaA and lpfO113. E. coli O22:H8 formed stronger biofilms in abiotic surface than E. coli O157:H7 (eae+, stx2+) and displayed a more adherent phenotype in vitro towards HeLa cells. Furthermore, when both serotypes were cultured together O22:H8 could reduce O157:H7 adherence in vitro. When calves were intragastrically pre-challenged with 108 CFU of a mixture of the three STEC strains and two days later challenged with the same dose of the strain E. coli O157:H7 438/99, the shedding of the pathogen was significantly reduced. Conclusions: These results suggest that E. coli O22:H8, a serotype rarely associated with human illness, might compete with O157:H7 at the bovine recto-anal junction, making non O157 carrying-calves less susceptible to O157:H7 colonization and shedding of the bacteria to the environment | ||
| 653 | |a ESCHERICHIA COLI | ||
| 653 | |a STEC | ||
| 653 | |a EHEC | ||
| 653 | |a BOVINE | ||
| 653 | |a COLONIZATION | ||
| 653 | |a ADHESION | ||
| 700 | 1 | |9 67271 |a Martorelli, L. |u Instituto de Patobiología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Argentina | |
| 700 | 1 | |9 67272 |a Albanese, A. |u Laboratorio de Fisiopatogenia, Departamento de Fisiología, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina | |
| 700 | 1 | |a Vilte, D. |u Instituto de Patobiología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Argentina |9 67273 | |
| 700 | 1 | |9 12817 |a Cantet, Rodolfo Juan Carlos |u Facultad de Agronomía, Universidad de Buenos Aires-CONICET, Buenos Aires 1427, Argentina | |
| 700 | 1 | |a Bentancor, A. |u Cátedra de Microbiología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires 1427, Argentina |9 67274 | |
| 700 | 1 | |a Zolezzi, G. |u Servicio Fisiopatogenia, INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires 1282, Argentina |9 67275 | |
| 700 | 1 | |a Chinen, I. |u Servicio Fisiopatogenia, INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires 1282, Argentina |9 67276 | |
| 700 | 1 | |9 67277 |a Ibarra, C. |u Laboratorio de Fisiopatogenia, Departamento de Fisiología, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina | |
| 773 | |t Veterinary Microbiology |g Vol.208 (2017), p.8-17, tbls., grafs. | ||
| 856 | |f 2017martorelli |i en reservorio |q application/pdf |u http://ri.agro.uba.ar/files/intranet/articulo/2017martorelli.pdf |x ARTI201806 | ||
| 856 | |z LINK AL EDITOR |u http://www.elsevier.com | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||