1,10 phenanthroline Amino Acid Sequence Article Aspartic Acid Proteases Caseinolytic activity Fruit Insulin Molecular Sequence Data Peptide mass fingerprinting Plant aspartic protease Purification Salpichroa origanifolia Salpichroin Solanaceae amino acid sequence anion exchange chromatography aspartic proteinase benzylsulfonyl fluoride casein chemistry chymosin controlled study edetic acid enzyme kinetics fruit hemoglobin insulin isolation and purification matrix assisted laser desorption ionization time of flight mass spectrometry molecular genetics