Bacillus subtilis Bacterial Proteins Coat Gene Deletion Genetic Complementation Test Germination Protein Multimerization SASP-E Spores Spores, Bacterial article asparagine autofluorescence bacterial gene bacterial protein bacterium colony calcium controlled study dipicolinic acid fructose gamma type small acid soluble protein genetic complementation glucose hexadecane nonhuman phenotype plasmid potassium chloride protein determination protein function