Datos convertidos
{
"id": "I19-R120-10915-98333",
"record_format": "dspace",
"spelling": [
"I19-R120-10915-983332020-10-27T04:07:42Z http:\/\/sedici.unlp.edu.ar\/handle\/10915\/98333 https:\/\/ri.conicet.gov.ar\/11336\/93853 https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1042\/CBI20100507 issn:1065-6995 Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations Anchordoquy, Juan Mateo Picco, Sebasti\u00e1n Julio Seoane, Anal\u00eda Isabel Anchordoquy, Juan Patricio Ponzinibbio, Mar\u00eda Virginia Mattioli, Guillermo Alberto Peral Garc\u00eda, Pilar Furnus, Cecilia Cristina 2011-05 2020-06-16T18:52:04Z en Biolog\u00eda Annexin Apoptosis Comet assay DNA damage Cumulus cells Zinc The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 \u03bcg\/ml (Zn1); 1.1 \u03bcg\/ml (Zn2) and 1.5 \u03bcg\/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dosedependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24\u00b116.05; Zn1: 1.13\u00b15.31; Zn2: 0.10\u00b10.36; Zn3: 0.017\u00b10.06). All treatments were statistically different from the control (P50.014 for Zn1; P,0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142\u00b10.07; Zn1: 0.109\u00b10.0328; Zn2:0.102\u00b10.013; Zn3: 0.0577\u00b10.019). Statistical differences were found between control and Zn1 (P=0.0308), control and Zn2 (P=0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P=0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC. Instituto de Gen\u00e9tica Veterinaria Articulo Preprint http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application\/pdf 593-597"
],
"institution": [
"Universidad Nacional de La Plata"
],
"institution_str": "I-19",
"repository_str": "R-120",
"collection": [
"SEDICI (UNLP)"
],
"language": [
"Ingl\u00e9s"
],
"topic": [
"Biolog\u00eda",
"Annexin",
"Apoptosis",
"Comet assay",
"DNA damage",
"Cumulus cells",
"Zinc"
],
"spellingShingle": [
"Biolog\u00eda",
"Annexin",
"Apoptosis",
"Comet assay",
"DNA damage",
"Cumulus cells",
"Zinc",
"Anchordoquy, Juan Mateo",
"Picco, Sebasti\u00e1n Julio",
"Seoane, Anal\u00eda Isabel",
"Anchordoquy, Juan Patricio",
"Ponzinibbio, Mar\u00eda Virginia",
"Mattioli, Guillermo Alberto",
"Peral Garc\u00eda, Pilar",
"Furnus, Cecilia Cristina",
"Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations"
],
"topic_facet": [
"Biolog\u00eda",
"Annexin",
"Apoptosis",
"Comet assay",
"DNA damage",
"Cumulus cells",
"Zinc"
],
"description": "The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 \u03bcg\/ml (Zn1); 1.1 \u03bcg\/ml (Zn2) and 1.5 \u03bcg\/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dosedependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24\u00b116.05; Zn1: 1.13\u00b15.31; Zn2: 0.10\u00b10.36; Zn3: 0.017\u00b10.06). All treatments were statistically different from the control (P50.014 for Zn1; P,0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142\u00b10.07; Zn1: 0.109\u00b10.0328; Zn2:0.102\u00b10.013; Zn3: 0.0577\u00b10.019). Statistical differences were found between control and Zn1 (P=0.0308), control and Zn2 (P=0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P=0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC.",
"format": [
"Articulo",
"Preprint"
],
"author": [
"Anchordoquy, Juan Mateo",
"Picco, Sebasti\u00e1n Julio",
"Seoane, Anal\u00eda Isabel",
"Anchordoquy, Juan Patricio",
"Ponzinibbio, Mar\u00eda Virginia",
"Mattioli, Guillermo Alberto",
"Peral Garc\u00eda, Pilar",
"Furnus, Cecilia Cristina"
],
"author_facet": [
"Anchordoquy, Juan Mateo",
"Picco, Sebasti\u00e1n Julio",
"Seoane, Anal\u00eda Isabel",
"Anchordoquy, Juan Patricio",
"Ponzinibbio, Mar\u00eda Virginia",
"Mattioli, Guillermo Alberto",
"Peral Garc\u00eda, Pilar",
"Furnus, Cecilia Cristina"
],
"author_sort": "Anchordoquy, Juan Mateo",
"title": "Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"title_short": "Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"title_full": "Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"title_fullStr": "Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"title_full_unstemmed": "Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"title_sort": "analysis of apoptosis and dna damage in bovine cumulus cells after exposure in vitro to different zinc concentrations",
"publishDate": [
"2011"
],
"url": [
"http:\/\/sedici.unlp.edu.ar\/handle\/10915\/98333",
"https:\/\/ri.conicet.gov.ar\/11336\/93853",
"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1042\/CBI20100507"
],
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"_version_": 1734863526096273408
}