Amino Acid Motifs 2 Amino Acid Sequence 2 Bcy1 2 Catalytic Domain 2 Cyclic AMP-Dependent Protein Kinase Catalytic Subunits 2 Cyclic AMP-Dependent Protein Kinase Type I 2 Cyclic AMP-Dependent Protein Kinases 2 Enzyme Activation 2 Escherichia coli 2 Isoenzymes 2 Molecular Sequence Data 2 Mutagenesis 2 PKB (protein kinase B)-activating kinase homologue (Pkh) 2 Phosphoinositide-dependent protein kinase 1 (PDK1) 2 Phosphorylation 2 Protein-Serine-Threonine Kinases 2 Saccharomyces cerevisiae 2 Saccharomyces cerevisiae Proteins 2 Tpk 2 Western blotting 2 article 2 cAMP-dependent protein kinase (PKA) 2 catalysis 2 culture medium 2 cyclic AMP dependent protein kinase 2 enzyme activation 2 enzyme active site 2 enzyme assay 2 enzyme phosphorylation 2 enzyme purification 2