Amino Acid Motifs 1 Amino Acid Sequence 1 Bcy1 1 Catalytic Domain 1 Cyclic AMP-Dependent Protein Kinase Catalytic Subunits 1 Cyclic AMP-Dependent Protein Kinase Type I 1 Cyclic AMP-Dependent Protein Kinases 1 Enzyme Activation 1 Escherichia coli 1 Isoenzymes 1 Molecular Sequence Data 1 Mutagenesis 1 PKB (protein kinase B)-activating kinase homologue (Pkh) 1 Phosphoinositide-dependent protein kinase 1 (PDK1) 1 Phosphorylation 1 Protein-Serine-Threonine Kinases 1 Saccharomyces cerevisiae 1 Saccharomyces cerevisiae Proteins 1 Tpk 1 Western blotting 1 article 1 cAMP-dependent protein kinase (PKA) 1 catalysis 1 culture medium 1 cyclic AMP dependent protein kinase 1 enzyme activation 1 enzyme active site 1 enzyme assay 1 enzyme phosphorylation 1 enzyme purification 1