Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)

In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β-hydroxysteroid dehydrogenase Type 1 (11β-HSD1). In the testes of the toad Rhinella a...

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Autores principales: Czuchlej, S.C., Volonteri, M.C., Regueira, E., Ceballos, N.R.
Formato: JOUR
Materias:
amphibians
androgens
glucocorticoids (GC)
steroidogenesis
testes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_24715638_v331_n1_p17_Czuchlej
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_24715638_v331_n1_p17_Czuchlej
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spelling todo:paper_24715638_v331_n1_p17_Czuchlej2023-10-03T16:42:26Z Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura) Czuchlej, S.C. Volonteri, M.C. Regueira, E. Ceballos, N.R. amphibians androgens glucocorticoids (GC) steroidogenesis testes In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β-hydroxysteroid dehydrogenase Type 1 (11β-HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β-HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone-induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17-hydroxylase, C17,20-lyase (Cyp450 c17) without affecting the 3β-hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450 c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450 c17 activity via a GR-mediated mechanism. © 2018 Wiley Periodicals, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_24715638_v331_n1_p17_Czuchlej
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic amphibians
androgens
glucocorticoids (GC)
steroidogenesis
testes
spellingShingle amphibians
androgens
glucocorticoids (GC)
steroidogenesis
testes
Czuchlej, S.C.
Volonteri, M.C.
Regueira, E.
Ceballos, N.R.
Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
topic_facet amphibians
androgens
glucocorticoids (GC)
steroidogenesis
testes
description In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β-hydroxysteroid dehydrogenase Type 1 (11β-HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β-HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone-induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17-hydroxylase, C17,20-lyase (Cyp450 c17) without affecting the 3β-hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450 c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450 c17 activity via a GR-mediated mechanism. © 2018 Wiley Periodicals, Inc.
format JOUR
author Czuchlej, S.C.
Volonteri, M.C.
Regueira, E.
Ceballos, N.R.
author_facet Czuchlej, S.C.
Volonteri, M.C.
Regueira, E.
Ceballos, N.R.
author_sort Czuchlej, S.C.
title Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_short Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_fullStr Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_sort effect of glucocorticoids on androgen biosynthesis in the testes of the toad rhinella arenarum (amphibia, anura)
url http://hdl.handle.net/20.500.12110/paper_24715638_v331_n1_p17_Czuchlej
work_keys_str_mv AT czuchlejsc effectofglucocorticoidsonandrogenbiosynthesisinthetestesofthetoadrhinellaarenarumamphibiaanura
AT volonterimc effectofglucocorticoidsonandrogenbiosynthesisinthetestesofthetoadrhinellaarenarumamphibiaanura
AT regueirae effectofglucocorticoidsonandrogenbiosynthesisinthetestesofthetoadrhinellaarenarumamphibiaanura
AT ceballosnr effectofglucocorticoidsonandrogenbiosynthesisinthetestesofthetoadrhinellaarenarumamphibiaanura
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