Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection
An estimated one third of the world's population is affected by latent tuberculosis (TB), which once active represents a leading cause of death among infectious diseases. Human immunodeficiency virus (HIV) infection is a main predisposing factor to TB reactivation. Individuals HIV-TB co-infecte...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Vecchione |
| Aporte de: |
| id |
todo:paper_20452322_v8_n1_p_Vecchione |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_20452322_v8_n1_p_Vecchione2023-10-03T16:38:29Z Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection Vecchione, M.B. Eiras, J. Suarez, G.V. Angerami, M.T. Marquez, C. Sued, O. Ben, G. Pérez, H.M. Gonzalez, D. Maidana, P. Mesch, V. Quiroga, M.F. Bruttomesso, A.C. An estimated one third of the world's population is affected by latent tuberculosis (TB), which once active represents a leading cause of death among infectious diseases. Human immunodeficiency virus (HIV) infection is a main predisposing factor to TB reactivation. Individuals HIV-TB co-infected develop a chronic state of inflammation associated with hypothalamic-pituitary-adrenal (HPA) axis dysregulation. This results in a hormonal imbalance, disturbing the physiological levels of cortisol and dehydroepiandrosterone (DHEA). DHEA and its oxygenated metabolites androstenediol (AED), androstenetriol (AET) and 7-oxo-DHEA are immunomodulatory compounds that may regulate physiopathology in HIV-TB co-infection. In order to study possible changes in plasma levels of these hormones, we developed an approach based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). To our knowledge, this represents the first report of their simultaneous measurement in HIV-TB individuals and the comparison with healthy donors, obtaining statistically higher plasma levels of DHEA, AET and 7-oxo-DHEA in patients. Moreover, we found that concentrations of 7-oxo-DHEA positively correlated with absolute CD4+ T cell counts, nadir CD4+ T cell values and with individuals who presented TB restricted to the lungs. This research contributes to understanding the role of these hormones in HIV-TB and emphasizes the importance of deepening their study in this context. © 2018 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Vecchione |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
An estimated one third of the world's population is affected by latent tuberculosis (TB), which once active represents a leading cause of death among infectious diseases. Human immunodeficiency virus (HIV) infection is a main predisposing factor to TB reactivation. Individuals HIV-TB co-infected develop a chronic state of inflammation associated with hypothalamic-pituitary-adrenal (HPA) axis dysregulation. This results in a hormonal imbalance, disturbing the physiological levels of cortisol and dehydroepiandrosterone (DHEA). DHEA and its oxygenated metabolites androstenediol (AED), androstenetriol (AET) and 7-oxo-DHEA are immunomodulatory compounds that may regulate physiopathology in HIV-TB co-infection. In order to study possible changes in plasma levels of these hormones, we developed an approach based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). To our knowledge, this represents the first report of their simultaneous measurement in HIV-TB individuals and the comparison with healthy donors, obtaining statistically higher plasma levels of DHEA, AET and 7-oxo-DHEA in patients. Moreover, we found that concentrations of 7-oxo-DHEA positively correlated with absolute CD4+ T cell counts, nadir CD4+ T cell values and with individuals who presented TB restricted to the lungs. This research contributes to understanding the role of these hormones in HIV-TB and emphasizes the importance of deepening their study in this context. © 2018 The Author(s). |
| format |
JOUR |
| author |
Vecchione, M.B. Eiras, J. Suarez, G.V. Angerami, M.T. Marquez, C. Sued, O. Ben, G. Pérez, H.M. Gonzalez, D. Maidana, P. Mesch, V. Quiroga, M.F. Bruttomesso, A.C. |
| spellingShingle |
Vecchione, M.B. Eiras, J. Suarez, G.V. Angerami, M.T. Marquez, C. Sued, O. Ben, G. Pérez, H.M. Gonzalez, D. Maidana, P. Mesch, V. Quiroga, M.F. Bruttomesso, A.C. Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| author_facet |
Vecchione, M.B. Eiras, J. Suarez, G.V. Angerami, M.T. Marquez, C. Sued, O. Ben, G. Pérez, H.M. Gonzalez, D. Maidana, P. Mesch, V. Quiroga, M.F. Bruttomesso, A.C. |
| author_sort |
Vecchione, M.B. |
| title |
Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| title_short |
Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| title_full |
Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| title_fullStr |
Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| title_full_unstemmed |
Determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during HIV-TB coinfection |
| title_sort |
determination of dehydroepiandrosterone and its biologically active oxygenated metabolites in human plasma evinces a hormonal imbalance during hiv-tb coinfection |
| url |
http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Vecchione |
| work_keys_str_mv |
AT vecchionemb determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT eirasj determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT suarezgv determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT angeramimt determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT marquezc determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT suedo determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT beng determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT perezhm determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT gonzalezd determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT maidanap determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT meschv determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT quirogamf determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection AT bruttomessoac determinationofdehydroepiandrosteroneanditsbiologicallyactiveoxygenatedmetabolitesinhumanplasmaevincesahormonalimbalanceduringhivtbcoinfection |
| _version_ |
1807319119124496384 |