Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)

In the present work, we analysed the male meiosis, the content and distribution of heterochromatin and the number and location of nucleolus organizing regions in Microtomus lunifer (Berg, 1900) by means of standard technique, C- and fluorescent bandings, and fluorescent in situ hybridization with an...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Poggio, M.G., Bressa, M.J., Papeschi, A.G.
Formato: JOUR
Materias:
Evolutionary trends
Hammacerinae
Hemiptera
M-chromosomes
Meiosis
RDNA-FISH
Reduviidae
Microtomus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19930771_v5_n1_p1_Poggio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_19930771_v5_n1_p1_Poggio
record_format dspace
spelling todo:paper_19930771_v5_n1_p1_Poggio2023-10-03T16:37:31Z Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae) Poggio, M.G. Bressa, M.J. Papeschi, A.G. Evolutionary trends Hammacerinae Hemiptera M-chromosomes Meiosis RDNA-FISH Reduviidae Hemiptera Microtomus Reduviidae In the present work, we analysed the male meiosis, the content and distribution of heterochromatin and the number and location of nucleolus organizing regions in Microtomus lunifer (Berg, 1900) by means of standard technique, C- and fluorescent bandings, and fluorescent in situ hybridization with an 18S rDNA probe. This species is the second one cytogenetically analysed within the Hammacerinae. Its male diploid chromosome number is 31 (2n=28+X 1X 2Y), including a minute pair of m-chromosomes. The diploid autosomal number and the presence of m-chromosomes are similar to those reported in M. conspicillaris (Drury, 1782) (2n=28+XY). However, M. lunifer has a multiple sex chromosome system X 1X 2Y (male) that could have originated by fragmentation of the ancestral X chromosome. Taking into account that M. conspicillaris and M. lunifer are the only two species within Reduviidae that possess m-chromosomes, the presence of this pair could be a synapomorphy for the species of this genus. C- and fluorescent bandings showed that the amount of heterochromatin in M. lunifer was small, and only a small CMA 3 bright band was observed in the largest autosomal pair at one terminal region. FISH with the 18S rDNA probe demonstrated that ribosomal genes were terminally placed on the largest autosomal pair. Our present results led us to propose that the location of rDNA genes could be associated with variants of the sex chromosome systems in relation with a kind of the sex chromosome systems within this family. Furthermore, the terminal location of NOR in the largest autosomal pair allowed us to use it as a chromosome marker and, thus, to infer that the kinetic activity of both ends is not a random process, and there is an inversion of this activity. © M.G. Poggio et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19930771_v5_n1_p1_Poggio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Evolutionary trends
Hammacerinae
Hemiptera
M-chromosomes
Meiosis
RDNA-FISH
Reduviidae
Hemiptera
Microtomus
Reduviidae
spellingShingle Evolutionary trends
Hammacerinae
Hemiptera
M-chromosomes
Meiosis
RDNA-FISH
Reduviidae
Hemiptera
Microtomus
Reduviidae
Poggio, M.G.
Bressa, M.J.
Papeschi, A.G.
Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
topic_facet Evolutionary trends
Hammacerinae
Hemiptera
M-chromosomes
Meiosis
RDNA-FISH
Reduviidae
Hemiptera
Microtomus
Reduviidae
description In the present work, we analysed the male meiosis, the content and distribution of heterochromatin and the number and location of nucleolus organizing regions in Microtomus lunifer (Berg, 1900) by means of standard technique, C- and fluorescent bandings, and fluorescent in situ hybridization with an 18S rDNA probe. This species is the second one cytogenetically analysed within the Hammacerinae. Its male diploid chromosome number is 31 (2n=28+X 1X 2Y), including a minute pair of m-chromosomes. The diploid autosomal number and the presence of m-chromosomes are similar to those reported in M. conspicillaris (Drury, 1782) (2n=28+XY). However, M. lunifer has a multiple sex chromosome system X 1X 2Y (male) that could have originated by fragmentation of the ancestral X chromosome. Taking into account that M. conspicillaris and M. lunifer are the only two species within Reduviidae that possess m-chromosomes, the presence of this pair could be a synapomorphy for the species of this genus. C- and fluorescent bandings showed that the amount of heterochromatin in M. lunifer was small, and only a small CMA 3 bright band was observed in the largest autosomal pair at one terminal region. FISH with the 18S rDNA probe demonstrated that ribosomal genes were terminally placed on the largest autosomal pair. Our present results led us to propose that the location of rDNA genes could be associated with variants of the sex chromosome systems in relation with a kind of the sex chromosome systems within this family. Furthermore, the terminal location of NOR in the largest autosomal pair allowed us to use it as a chromosome marker and, thus, to infer that the kinetic activity of both ends is not a random process, and there is an inversion of this activity. © M.G. Poggio et al.
format JOUR
author Poggio, M.G.
Bressa, M.J.
Papeschi, A.G.
author_facet Poggio, M.G.
Bressa, M.J.
Papeschi, A.G.
author_sort Poggio, M.G.
title Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
title_short Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
title_full Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
title_fullStr Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
title_full_unstemmed Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)
title_sort male meiosis, heterochromatin characterization and chromosomal location of rdna in microtomus lunifer (berg, 1900) (hemiptera: reduviidae: hammacerinae)
url http://hdl.handle.net/20.500.12110/paper_19930771_v5_n1_p1_Poggio
work_keys_str_mv AT poggiomg malemeiosisheterochromatincharacterizationandchromosomallocationofrdnainmicrotomusluniferberg1900hemipterareduviidaehammacerinae
AT bressamj malemeiosisheterochromatincharacterizationandchromosomallocationofrdnainmicrotomusluniferberg1900hemipterareduviidaehammacerinae
AT papeschiag malemeiosisheterochromatincharacterizationandchromosomallocationofrdnainmicrotomusluniferberg1900hemipterareduviidaehammacerinae
_version_ 1807323672545853440

Ejemplares similares