Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and pre...
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todo:paper_19326203_v11_n7_p_Carreira2023-10-03T16:34:43Z Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster Carreira, V.P. Mensch, J. Hasson, E. Fanara, J.J. Argentina chromosome substitution strain Drosophila melanogaster gene frequency gene mutation genetic variation human human experiment morphological trait natural population phenotype quantitative trait anatomy and histology animal Drosophila ecosystem gene genetic polymorphism genetics quantitative trait locus Animals Argentina Drosophila Ecosystem Genes, Insect Phenotype Polymorphism, Genetic Quantitative Trait Loci Quantitative Trait, Heritable Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and previous work identified genomic regions associated with such variation in D. melanogaster. However, the genetic factors that orchestrate morphological variation have been barely studied. Here, our main objective was to investigate genetic variation for different morphological traits associated to the second chromosome in natural populations of D. melanogaster along latitudinal and altitudinal gradients in Argentina. Our results revealed weak clinal signals and a strong population effect on morphological variation. Moreover, most pairwise comparisons between populations were significant. Our study also showed important within-population genetic variation, which must be associated to the second chromosome, as the lines are otherwise genetically identical. Next, we examined the contribution of different candidate genes to natural variation for these traits. We performed quantitative complementation tests using a battery of lines bearing mutated alleles at candidate genes located in the second chromosome and six second chromosome substitution lines derived from natural populations which exhibited divergent phenotypes. Results of complementation tests revealed that natural variation at all candidate genes studied, invected, Fasciclin 3, toucan, Reticulon-like1, jing and CG14478, affects the studied characters, suggesting that they are Quantitative Trait Genes for morphological traits. Finally, the phenotypic patterns observed suggest that different alleles of each gene might contribute to natural variation for morphological traits. However, non-additive effects cannot be ruled out, as wild-derived strains differ at myriads of second chromosome loci that may interact epistatically with mutant alleles.Copyright: © 2016 Carreira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Carreira, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v11_n7_p_Carreira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Argentina chromosome substitution strain Drosophila melanogaster gene frequency gene mutation genetic variation human human experiment morphological trait natural population phenotype quantitative trait anatomy and histology animal Drosophila ecosystem gene genetic polymorphism genetics quantitative trait locus Animals Argentina Drosophila Ecosystem Genes, Insect Phenotype Polymorphism, Genetic Quantitative Trait Loci Quantitative Trait, Heritable |
| spellingShingle |
Argentina chromosome substitution strain Drosophila melanogaster gene frequency gene mutation genetic variation human human experiment morphological trait natural population phenotype quantitative trait anatomy and histology animal Drosophila ecosystem gene genetic polymorphism genetics quantitative trait locus Animals Argentina Drosophila Ecosystem Genes, Insect Phenotype Polymorphism, Genetic Quantitative Trait Loci Quantitative Trait, Heritable Carreira, V.P. Mensch, J. Hasson, E. Fanara, J.J. Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| topic_facet |
Argentina chromosome substitution strain Drosophila melanogaster gene frequency gene mutation genetic variation human human experiment morphological trait natural population phenotype quantitative trait anatomy and histology animal Drosophila ecosystem gene genetic polymorphism genetics quantitative trait locus Animals Argentina Drosophila Ecosystem Genes, Insect Phenotype Polymorphism, Genetic Quantitative Trait Loci Quantitative Trait, Heritable |
| description |
Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and previous work identified genomic regions associated with such variation in D. melanogaster. However, the genetic factors that orchestrate morphological variation have been barely studied. Here, our main objective was to investigate genetic variation for different morphological traits associated to the second chromosome in natural populations of D. melanogaster along latitudinal and altitudinal gradients in Argentina. Our results revealed weak clinal signals and a strong population effect on morphological variation. Moreover, most pairwise comparisons between populations were significant. Our study also showed important within-population genetic variation, which must be associated to the second chromosome, as the lines are otherwise genetically identical. Next, we examined the contribution of different candidate genes to natural variation for these traits. We performed quantitative complementation tests using a battery of lines bearing mutated alleles at candidate genes located in the second chromosome and six second chromosome substitution lines derived from natural populations which exhibited divergent phenotypes. Results of complementation tests revealed that natural variation at all candidate genes studied, invected, Fasciclin 3, toucan, Reticulon-like1, jing and CG14478, affects the studied characters, suggesting that they are Quantitative Trait Genes for morphological traits. Finally, the phenotypic patterns observed suggest that different alleles of each gene might contribute to natural variation for morphological traits. However, non-additive effects cannot be ruled out, as wild-derived strains differ at myriads of second chromosome loci that may interact epistatically with mutant alleles.Copyright: © 2016 Carreira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| format |
JOUR |
| author |
Carreira, V.P. Mensch, J. Hasson, E. Fanara, J.J. |
| author_facet |
Carreira, V.P. Mensch, J. Hasson, E. Fanara, J.J. |
| author_sort |
Carreira, V.P. |
| title |
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| title_short |
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| title_full |
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| title_fullStr |
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| title_full_unstemmed |
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| title_sort |
natural genetic variation and candidate genes for morphological traits in drosophila melanogaster |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v11_n7_p_Carreira |
| work_keys_str_mv |
AT carreiravp naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster AT menschj naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster AT hassone naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster AT fanarajj naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster |
| _version_ |
1807322125155958784 |