In vitro antibacterial effect of exotic plants essential oils on the honeybee pathogen Paenibacillus larvae, causal agent of American foulbrood

Chemical composition and antimicrobial activity of exotic plants essential oils to potentially control Paenibacillus larvae, the causal agent of American foulbrood disease (AFB) were determined. AFB represents one of the main plagues that affect the colonies of honeybees Apis mellifera L. with high...

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Detalles Bibliográficos
Autores principales: Fuselli, S.R., García de la Rosa, S.B., Eguaras, M.J., Fritz, R.
Formato: JOUR
Lenguaje:Spanish
Materias:
Antimicrobial activity
Chemical composition
Cymbopogon martini
Cymbopogon nardus
Honeybees' pathogen
Melaleuca alternifolia
Melaleuca viridiflora
Apis mellifera
Bacteria (microorganisms)
Cymbopogon
Cymbopogon martinii
Melaleuca
Melaleuca quinquenervia
Myrtaceae
Paenibacillus
Paenibacillus larvae
Poaceae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1695971X_v8_n3_p651_Fuselli
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Chemical composition and antimicrobial activity of exotic plants essential oils to potentially control Paenibacillus larvae, the causal agent of American foulbrood disease (AFB) were determined. AFB represents one of the main plagues that affect the colonies of honeybees Apis mellifera L. with high negative impact on beekeepers worldwide. Essential oils tested were niaouli (Melaleuca viridiflora) and tea tree (Melaleuca alternifolia) from Myrtaceae, and citronella grass (Cymbopogon nardus) and palmarosa (Cymbopogon martinii) from Gramineae. The components of the essential oils were identified by SPME-GC/MS analysis. The antimicrobial activity of the oils against P. larvae was determined by the broth microdilution method. In vitro assays of M. viridiflora and C. nardus oils showed the inhibition of the bacterial strains at the lowest concentrations tested, with minimal inhibitory concentration (MIC) mean value about 320 mg L-1 for both oils, respectively. This property could be attributed to the kind and percentage of the components of the oils. Terpinen-4-ol (29.09%), α-pinene (21.63%) and limonene (17.4%) were predominant in M. viridiflora, while limonene (24.74%), citronelal (24.61%) and geraniol (15.79%) were the bulk of C. nardus. The use of these essential oils contributes to the screening of alternative natural compounds to control AFB in the apiaries; toxicological risks and other undesirable effects would be avoided as resistance factors, developed by the indiscriminate use of antibiotics.

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