Fluorescence imaging of amyloid formation in living cells by a functional, tetracysteine-tagged α-synuclein

α-synuclein is a major component of intraneuronal protein aggregates constituting a distinctive feature of Parkinson disease. To date, fluorescence imaging of dynamic processes leading to such amyloid deposits in living cells has not been feasible. To address this need, we generated a recombinant α-...

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Detalles Bibliográficos
Autores principales: Roberti, M.J., Bertoncini, C.W., Klement, R., Jares-Erijman, E.A., Jovin, T.M.
Formato: JOUR
Materias:
alpha synuclein
amyloid
cysteine
fluorescent dye
hybrid protein
reactive oxygen metabolite
article
cell stress
fluorescence analysis
fluorescence resonance energy transfer
oxidative stress
Parkinson disease
priority journal
protein aggregation
protein protein interaction
chemistry
confocal microscopy
Escherichia coli
fluorescence microscopy
gene vector
genetic procedures
genetic transfection
genetics
human
metabolism
methodology
tumor cell line
alpha-Synuclein
Amyloid
Biosensing Techniques
Cell Line, Tumor
Cysteine
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
Genetic Vectors
Humans
Microscopy, Confocal
Microscopy, Fluorescence
Oxidative Stress
Reactive Oxygen Species
Recombinant Fusion Proteins
Transfection
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15487091_v4_n4_p345_Roberti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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