Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors

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Intracellular transport of large cargoes, such as organelles, vesicles, or large proteins, is a complex dynamical process that involves the interplay of adenosine triphosphate-consuming molecular motors, cytoskeleton filaments, and the viscoelastic cytoplasm. In this work we investigate the motion o...

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Autores principales: Bruno, L., Levi, V., Brunstein, M., Despósito, M.A.
Formato: JOUR
Materias:
Active transport
Analytical expressions
Collective action
Cytoskeleton filaments
Dynamical process
Experimental data
Generalized Langevin equation
Intracellular transport
Living cell
Mean-square displacement
Molecular motors
Quantitative description
Spatio-temporal resolution
Theoretical expression
Theoretical models
Tracking techniques
Xenopus laevis
Adenosinetriphosphate
Cytology
Equations of motion
Linear motors
Stochastic models
Motors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15393755_v80_n1_p_Bruno
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_15393755_v80_n1_p_Bruno
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spelling todo:paper_15393755_v80_n1_p_Bruno2023-10-03T16:22:27Z Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors Bruno, L. Levi, V. Brunstein, M. Despósito, M.A. Active transport Analytical expressions Collective action Cytoskeleton filaments Dynamical process Experimental data Generalized Langevin equation Intracellular transport Living cell Mean-square displacement Molecular motors Quantitative description Spatio-temporal resolution Theoretical expression Theoretical models Tracking techniques Xenopus laevis Adenosinetriphosphate Cytology Equations of motion Linear motors Stochastic models Motors Intracellular transport of large cargoes, such as organelles, vesicles, or large proteins, is a complex dynamical process that involves the interplay of adenosine triphosphate-consuming molecular motors, cytoskeleton filaments, and the viscoelastic cytoplasm. In this work we investigate the motion of pigment organelles (melanosomes) driven by myosin-V motors in Xenopus laevis melanocytes using a high-spatio-temporal resolution tracking technique. By analyzing the obtained trajectories, we show that the melanosomes mean-square displacement undergoes a transition from a subdiffusive to a superdiffusive behavior. A stochastic theoretical model, which explicitly considers the collective action of the molecular motors, is introduced to generalize the interpretation of our data. Starting from a generalized Langevin equation, we derive an analytical expression for the mean square displacement, which also takes into account the experimental noise. By fitting theoretical expressions to experimental data we were able to discriminate the exponents that characterize the passive and active contributions to the dynamics and to estimate the "global" motor forces correctly. Then, our model gives a quantitative description of active transport in living cells with a reduced number of parameters. © 2009 The American Physical Society. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15393755_v80_n1_p_Bruno
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Active transport
Analytical expressions
Collective action
Cytoskeleton filaments
Dynamical process
Experimental data
Generalized Langevin equation
Intracellular transport
Living cell
Mean-square displacement
Molecular motors
Quantitative description
Spatio-temporal resolution
Theoretical expression
Theoretical models
Tracking techniques
Xenopus laevis
Adenosinetriphosphate
Cytology
Equations of motion
Linear motors
Stochastic models
Motors
spellingShingle Active transport
Analytical expressions
Collective action
Cytoskeleton filaments
Dynamical process
Experimental data
Generalized Langevin equation
Intracellular transport
Living cell
Mean-square displacement
Molecular motors
Quantitative description
Spatio-temporal resolution
Theoretical expression
Theoretical models
Tracking techniques
Xenopus laevis
Adenosinetriphosphate
Cytology
Equations of motion
Linear motors
Stochastic models
Motors
Bruno, L.
Levi, V.
Brunstein, M.
Despósito, M.A.
Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
topic_facet Active transport
Analytical expressions
Collective action
Cytoskeleton filaments
Dynamical process
Experimental data
Generalized Langevin equation
Intracellular transport
Living cell
Mean-square displacement
Molecular motors
Quantitative description
Spatio-temporal resolution
Theoretical expression
Theoretical models
Tracking techniques
Xenopus laevis
Adenosinetriphosphate
Cytology
Equations of motion
Linear motors
Stochastic models
Motors
description Intracellular transport of large cargoes, such as organelles, vesicles, or large proteins, is a complex dynamical process that involves the interplay of adenosine triphosphate-consuming molecular motors, cytoskeleton filaments, and the viscoelastic cytoplasm. In this work we investigate the motion of pigment organelles (melanosomes) driven by myosin-V motors in Xenopus laevis melanocytes using a high-spatio-temporal resolution tracking technique. By analyzing the obtained trajectories, we show that the melanosomes mean-square displacement undergoes a transition from a subdiffusive to a superdiffusive behavior. A stochastic theoretical model, which explicitly considers the collective action of the molecular motors, is introduced to generalize the interpretation of our data. Starting from a generalized Langevin equation, we derive an analytical expression for the mean square displacement, which also takes into account the experimental noise. By fitting theoretical expressions to experimental data we were able to discriminate the exponents that characterize the passive and active contributions to the dynamics and to estimate the "global" motor forces correctly. Then, our model gives a quantitative description of active transport in living cells with a reduced number of parameters. © 2009 The American Physical Society.
format JOUR
author Bruno, L.
Levi, V.
Brunstein, M.
Despósito, M.A.
author_facet Bruno, L.
Levi, V.
Brunstein, M.
Despósito, M.A.
author_sort Bruno, L.
title Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
title_short Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
title_full Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
title_fullStr Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
title_full_unstemmed Transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
title_sort transition to superdiffusive behavior in intracellular actin-based transport mediated by molecular motors
url http://hdl.handle.net/20.500.12110/paper_15393755_v80_n1_p_Bruno
work_keys_str_mv AT brunol transitiontosuperdiffusivebehaviorinintracellularactinbasedtransportmediatedbymolecularmotors
AT leviv transitiontosuperdiffusivebehaviorinintracellularactinbasedtransportmediatedbymolecularmotors
AT brunsteinm transitiontosuperdiffusivebehaviorinintracellularactinbasedtransportmediatedbymolecularmotors
AT despositoma transitiontosuperdiffusivebehaviorinintracellularactinbasedtransportmediatedbymolecularmotors
_version_ 1807321622981378048

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