Modulation of a photoswitchable dual-color quantum dot containing a photochromic FRET acceptor and an internal standard

Photoswitchable semiconductor nanoparticles, quantum dots (QDs), couple the advantages of conventional QDs with the ability to reversibly modulate the QD emission, thereby improving signal detection by rejection of background signals. Using a simple coating methodology with polymers incorporating a...

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Detalles Bibliográficos
Autores principales: Díaz, S.A., Giordano, L., Jovin, T.M., Jares-Erijman, E.A.
Formato: JOUR
Materias:
FRET
internal standard
photochromism
Quantum dots
ratiometric imaging
Background signals
Dual emissions
Fluorescence intensity ratio
Internal standards
Organic fluorophores
Photo-switchable
QD emissions
Ratiometric
Ratiometric imaging
Semiconductor nanoparticles
Organic polymers
Photochromism
Semiconductor quantum dots
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15306984_v12_n7_p3537_Diaz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Photoswitchable semiconductor nanoparticles, quantum dots (QDs), couple the advantages of conventional QDs with the ability to reversibly modulate the QD emission, thereby improving signal detection by rejection of background signals. Using a simple coating methodology with polymers incorporating a diheteroarylethene photochromic FRET acceptor as well as a spectrally distinct organic fluorophore, photoswitchable QDs were prepared that are small, biocompatible, and feature ratiometric dual emission. With programmed irradiation, the fluorescence intensity ratio can be modified by up to ∼100%. © 2012 American Chemical Society.

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