Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity

We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein gen...

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Detalles Bibliográficos
Autores principales: Clemente, M., Curilovic, R., Sassone, A., Zelada, A., Angel, S.O., Mentaberry, A.N.
Formato: JOUR
Materias:
Plant transient expression
PVX amplicon
Toxoplasma gondii
Bacteria
Diseases
Immunology
Plants (botany)
Tobacco
Viruses
Subgenomic promoters
Antigens
coat protein
immunoglobulin G2a
membrane antigen
plant extract
signal peptide
surface antigen 1
Toxoplasma gondii vaccine
unclassified drug
virus vector
amplicon
animal experiment
animal model
animal tissue
antigen expression
antigen specificity
apoplast
blood sampling
body burden
brain cyst
controlled study
female
functional genomics
gene construct
gene expression regulation
gene expression system
gene fusion
genetic strain
heterozygote
human
humoral immunity
immunization
immunogenicity
lethality
motion
mouse
nonhuman
plant leaf
plant tissue
plant virus
potato virus x
promoter region
provocation test
review
Rhizobium
solubility
tobacco
vaccination
viral gene delivery system
Western blotting
Animals
Antibodies, Protozoan
Antigens, Protozoan
Brain
Cysts
Female
Gene Expression
Immunoglobulin G
Mice
Mice, Inbred C3H
Plant Leaves
Potexvirus
Protozoan Proteins
Toxoplasma
Toxoplasmosis
Genes
Plants
Agrobacterium
Nicotiana tabacum
Potato virus X
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10736085_v30_n1_p41_Clemente
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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