Heterogeneous distribution of histone methylation in mature human sperm

Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We ob...

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Autores principales: La Spina, F.A., Romanato, M., Brugo-Olmedo, S., De Vincentiis, S., Julianelli, V., Rivera, R.M., Buffone, M.G.
Formato: JOUR
Materias:
Epigenetic
Histone modifications
Human sperm
Sperm subpopulations
histone
article
cell differentiation
cell structure
controlled study
ejaculation
HeLa cell
histone acetylation
histone methylation
histone modification
human
human cell
immunocytochemistry
immunofluorescence
male
normal human
nucleosome
priority journal
prospective study
protein processing
semen analysis
spermatid
spermatozoon
spermatozoon maturation
Cell Nucleus
Epigenesis, Genetic
HeLa Cells
Histone-Lysine N-Methyltransferase
Histones
Humans
Male
Methylation
Semen Analysis
Spermatogenesis
Spermatozoa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10580468_v31_n1_p45_LaSpina
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10580468_v31_n1_p45_LaSpina
record_format dspace
spelling todo:paper_10580468_v31_n1_p45_LaSpina2023-10-03T16:00:55Z Heterogeneous distribution of histone methylation in mature human sperm La Spina, F.A. Romanato, M. Brugo-Olmedo, S. De Vincentiis, S. Julianelli, V. Rivera, R.M. Buffone, M.G. Epigenetic Histone modifications Human sperm Sperm subpopulations histone article cell differentiation cell structure controlled study ejaculation HeLa cell histone acetylation histone methylation histone modification human human cell immunocytochemistry immunofluorescence male normal human nucleosome priority journal prospective study protein processing semen analysis spermatid spermatozoon spermatozoon maturation Cell Nucleus Epigenesis, Genetic HeLa Cells Histone-Lysine N-Methyltransferase Histones Humans Male Methylation Semen Analysis Spermatogenesis Spermatozoa Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm. Conclusions: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility. © 2013 Springer Science+Business Media. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10580468_v31_n1_p45_LaSpina
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Epigenetic
Histone modifications
Human sperm
Sperm subpopulations
histone
article
cell differentiation
cell structure
controlled study
ejaculation
HeLa cell
histone acetylation
histone methylation
histone modification
human
human cell
immunocytochemistry
immunofluorescence
male
normal human
nucleosome
priority journal
prospective study
protein processing
semen analysis
spermatid
spermatozoon
spermatozoon maturation
Cell Nucleus
Epigenesis, Genetic
HeLa Cells
Histone-Lysine N-Methyltransferase
Histones
Humans
Male
Methylation
Semen Analysis
Spermatogenesis
Spermatozoa
spellingShingle Epigenetic
Histone modifications
Human sperm
Sperm subpopulations
histone
article
cell differentiation
cell structure
controlled study
ejaculation
HeLa cell
histone acetylation
histone methylation
histone modification
human
human cell
immunocytochemistry
immunofluorescence
male
normal human
nucleosome
priority journal
prospective study
protein processing
semen analysis
spermatid
spermatozoon
spermatozoon maturation
Cell Nucleus
Epigenesis, Genetic
HeLa Cells
Histone-Lysine N-Methyltransferase
Histones
Humans
Male
Methylation
Semen Analysis
Spermatogenesis
Spermatozoa
La Spina, F.A.
Romanato, M.
Brugo-Olmedo, S.
De Vincentiis, S.
Julianelli, V.
Rivera, R.M.
Buffone, M.G.
Heterogeneous distribution of histone methylation in mature human sperm
topic_facet Epigenetic
Histone modifications
Human sperm
Sperm subpopulations
histone
article
cell differentiation
cell structure
controlled study
ejaculation
HeLa cell
histone acetylation
histone methylation
histone modification
human
human cell
immunocytochemistry
immunofluorescence
male
normal human
nucleosome
priority journal
prospective study
protein processing
semen analysis
spermatid
spermatozoon
spermatozoon maturation
Cell Nucleus
Epigenesis, Genetic
HeLa Cells
Histone-Lysine N-Methyltransferase
Histones
Humans
Male
Methylation
Semen Analysis
Spermatogenesis
Spermatozoa
description Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm. Conclusions: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility. © 2013 Springer Science+Business Media.
format JOUR
author La Spina, F.A.
Romanato, M.
Brugo-Olmedo, S.
De Vincentiis, S.
Julianelli, V.
Rivera, R.M.
Buffone, M.G.
author_facet La Spina, F.A.
Romanato, M.
Brugo-Olmedo, S.
De Vincentiis, S.
Julianelli, V.
Rivera, R.M.
Buffone, M.G.
author_sort La Spina, F.A.
title Heterogeneous distribution of histone methylation in mature human sperm
title_short Heterogeneous distribution of histone methylation in mature human sperm
title_full Heterogeneous distribution of histone methylation in mature human sperm
title_fullStr Heterogeneous distribution of histone methylation in mature human sperm
title_full_unstemmed Heterogeneous distribution of histone methylation in mature human sperm
title_sort heterogeneous distribution of histone methylation in mature human sperm
url http://hdl.handle.net/20.500.12110/paper_10580468_v31_n1_p45_LaSpina
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AT romanatom heterogeneousdistributionofhistonemethylationinmaturehumansperm
AT brugoolmedos heterogeneousdistributionofhistonemethylationinmaturehumansperm
AT devincentiiss heterogeneousdistributionofhistonemethylationinmaturehumansperm
AT julianelliv heterogeneousdistributionofhistonemethylationinmaturehumansperm
AT riverarm heterogeneousdistributionofhistonemethylationinmaturehumansperm
AT buffonemg heterogeneousdistributionofhistonemethylationinmaturehumansperm
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