X-Y sample scanning stage and calibration method suitable for single-molecule detection

This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the typ...

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Detalles Bibliográficos
Autores principales: Treegate, K., Rasamessard, A., Osotchan, T., Hodak, J.H.
Formato: JOUR
Materias:
Fluorescence spectroscopy
Optical detection
Scanning confocal microscopy
Single molecule spectroscopy
Aluminum plates
Calibration method
Correction techniques
Cut out
Diffraction-limited resolution
Direct calibration
Dwell time
Fluorescent dyes
Focus adjustment
Imaging glass
Line profiles
Mechanical response
Microscope objective
Positioning devices
Range images
Scanning confocal microscopes
Scanning device
Scanning stages
Single molecule fluorescence
Single-molecule detection
Target molecule
Calibration
Confocal microscopy
Fluorescence
Microscopes
Piezoelectric actuators
Scanning
Molecules
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09254005_v150_n1_p239_Treegate
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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