Cytogenetic studies in species of passiflora subgenus passiflora (passifloraceae)

Passifora has nearly 525 spp. with x = 9 or 6. A cytogenetic study of four species from subgenus Passifora that grow in Argentina was performed. Giemsa technique, fuorescent chromosome banding CMA/DAPI and FISH (probe 18-5,8-26S and 5S) were used in mitotic chromosomes. P. caerulea, P. tucumanensis...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Chiapero, A.L., Peñas, M.L.L., García, M.T.A., Bernardello, G.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0373580X_v48_n1_p103_Chiapero
Aporte de:
Descripción
Sumario:Passifora has nearly 525 spp. with x = 9 or 6. A cytogenetic study of four species from subgenus Passifora that grow in Argentina was performed. Giemsa technique, fuorescent chromosome banding CMA/DAPI and FISH (probe 18-5,8-26S and 5S) were used in mitotic chromosomes. P. caerulea, P. tucumanensis and P. cincinnata resulted diploid (2n = 18) while P. mooreana tetraploid (2n = 36). The karyotypes were highly symmetrical, with the formula 8 m + 1 sm- , duplicated in P. mooreana. With CMA/DAPI, diploids showed 2-3 pairs of terminal bands CMA /DAPI , whereas the tetraploid fve bands, associated t+o NOR-in all cases. With FISH, the loci 18-5.8-26S coincided in number and position with the CMA /DAPI bands. In each species, the number of sites for 5S rDNA genes agreed with the ploidy level. Data of P. tucumanensis and P. mooreana are here reported for the frst time, being this last species the frst known tetraploid for subgenus Passifora. FISH patterns and heterochromatic bands varied among the Passifora species studied, mainly with respect to the number and distribution of the signals.