SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus

Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Solu...

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Detalles Bibliográficos
Autores principales: Cucchi, A., De Rivas, C.S.
Formato: JOUR
Materias:
bacterial protein
amino acid sequence
article
bacillus sphaericus
bacillus thuringiensis
bacterial growth
environmental factor
nonhuman
osmoregulation
priority journal
protein determination
sporogenesis
stress
Bacillus
Bacillus thuringiensis
Bacterial Proteins
Osmotic Pressure
Peroxides
Sodium Chloride
Spores, Bacterial
Temperature
Ultraviolet Rays
Bacilli
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Posibacteria
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03438651_v36_n4_p220_Cucchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Soluble Spore Proteins), α/β and γ type, are involved in spore tolerance to heat, UV light, peroxide, and salt, they were analyzed in Bti and Bf. The molecular weight, migration pattern and amino acid composition of different SASP were determined and compared with other bacilli, in particular to B. subtilis. A relation between spore osmotolerance, SASP content, and amino acid composition was shown. In addition, the absence of τ SASP in Bti and Bf is discussed.

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