Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes

Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results...

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Detalles Bibliográficos
Autores principales: Llanos, S.L., Roldán, A.
Formato: JOUR
Materias:
Astrocytes
Dexamethasone
Macrophages
NADPH-diaphorase
NO·
Phagocytosis
dexamethasone
glucocorticoid
isoenzyme
nitric oxide
nitric oxide synthase
reduced nicotinamide adenine dinucleotide phosphate dehydrogenase
zymosan
animal cell
article
astrocyte
controlled study
enzyme activation
enzyme activity
hormonal regulation
newborn
nonhuman
peritoneum macrophage
phagocytosis
protein expression
rat
Animals
Animals, Newborn
Cells, Cultured
Cerebral Cortex
Glucocorticoids
Nitric Oxide
Rats
Rats, Sprague-Dawley
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03279545_v23_n1_p29_Llanos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results corroborate the presence of both constitutive and induced NO· synthase (cNOS and iNOS) in astrocytes, while macrophages express only the inducible isoform. Dexamethasone was able to decrease NO· synthesis produced by induction of iNOS in macrophage and astrocyte phagocytosis, but not the NO· produced by the cNOS spontaneously expressed in cultured astrocytes.

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