Midazolam disrupts fear memory reconsolidation
The current research examines the influence of midazolam (MDZ) on memory reconsolidation using a contextual fear paradigm in rats, based on three context-shock training trials (0.7 mA, 3 s). First, we evaluate the effect of MDZ (1 mg/kg, i.p.) injected shortly after the training procedure. Second, w...
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todo:paper_03064522_v139_n3_p831_Bustos2023-10-03T15:22:22Z Midazolam disrupts fear memory reconsolidation Bustos, S.G. Maldonado, H. Molina, V.A. benzodiazepine contextual aversive conditioning GABA-A receptors reminder retrograde amnesia 4 aminobutyric acid A receptor midazolam benzodiazepine receptor affecting agent midazolam animal experiment article conditioning controlled study drug effect environmental factor fear freezing male memory consolidation nonhuman photoreactivation priority journal rat scoring system stimulus response training animal brain conditioned reflex electric shock learning long term memory memory physiology time Wistar rat Animals Association Learning Brain Conditioning, Classical Electroshock Fear GABA Modulators Male Memory Midazolam Rats Rats, Wistar Retention (Psychology) Time Factors The current research examines the influence of midazolam (MDZ) on memory reconsolidation using a contextual fear paradigm in rats, based on three context-shock training trials (0.7 mA, 3 s). First, we evaluate the effect of MDZ (1 mg/kg, i.p.) injected shortly after the training procedure. Second, we examined the influence of MDZ after a brief exposure (90 s) either in the training context (reactivation procedure) or in a neutral environment (no reactivation procedure) and one day later, freezing behavior was scored when rats were re-exposed to the training environment. Third, we investigate both the effect of MDZ administered at different times following reactivation on fear memory and the persistence of such effect 10 days after reactivation. Finally, we test whether the MDZ effect could be reverted by a single weak training trial (0.2 mA, 3 s) or by the presentation of the same unconditioned stimulus in the absence of the conditioned stimulus as a reminder which proves to induce significant freezing in rats not previously trained. Results show that MDZ interferes with the formation of a contextual fear memory only when administered after the reactivation procedure but not after the training procedure. This interference was effective up to 60 min after reactivation and not at a later time. No spontaneous recovery of freezing behavior was observed 11 days after MDZ injection which was not reverted by a weak training trial and by the unconditioned stimulus alone. All these data support the idea that stimulating GABA A receptor sites via MDZ selectively disrupts the reconsolidation process of a contextual fear memory. © 2006 IBRO. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03064522_v139_n3_p831_Bustos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
benzodiazepine contextual aversive conditioning GABA-A receptors reminder retrograde amnesia 4 aminobutyric acid A receptor midazolam benzodiazepine receptor affecting agent midazolam animal experiment article conditioning controlled study drug effect environmental factor fear freezing male memory consolidation nonhuman photoreactivation priority journal rat scoring system stimulus response training animal brain conditioned reflex electric shock learning long term memory memory physiology time Wistar rat Animals Association Learning Brain Conditioning, Classical Electroshock Fear GABA Modulators Male Memory Midazolam Rats Rats, Wistar Retention (Psychology) Time Factors |
| spellingShingle |
benzodiazepine contextual aversive conditioning GABA-A receptors reminder retrograde amnesia 4 aminobutyric acid A receptor midazolam benzodiazepine receptor affecting agent midazolam animal experiment article conditioning controlled study drug effect environmental factor fear freezing male memory consolidation nonhuman photoreactivation priority journal rat scoring system stimulus response training animal brain conditioned reflex electric shock learning long term memory memory physiology time Wistar rat Animals Association Learning Brain Conditioning, Classical Electroshock Fear GABA Modulators Male Memory Midazolam Rats Rats, Wistar Retention (Psychology) Time Factors Bustos, S.G. Maldonado, H. Molina, V.A. Midazolam disrupts fear memory reconsolidation |
| topic_facet |
benzodiazepine contextual aversive conditioning GABA-A receptors reminder retrograde amnesia 4 aminobutyric acid A receptor midazolam benzodiazepine receptor affecting agent midazolam animal experiment article conditioning controlled study drug effect environmental factor fear freezing male memory consolidation nonhuman photoreactivation priority journal rat scoring system stimulus response training animal brain conditioned reflex electric shock learning long term memory memory physiology time Wistar rat Animals Association Learning Brain Conditioning, Classical Electroshock Fear GABA Modulators Male Memory Midazolam Rats Rats, Wistar Retention (Psychology) Time Factors |
| description |
The current research examines the influence of midazolam (MDZ) on memory reconsolidation using a contextual fear paradigm in rats, based on three context-shock training trials (0.7 mA, 3 s). First, we evaluate the effect of MDZ (1 mg/kg, i.p.) injected shortly after the training procedure. Second, we examined the influence of MDZ after a brief exposure (90 s) either in the training context (reactivation procedure) or in a neutral environment (no reactivation procedure) and one day later, freezing behavior was scored when rats were re-exposed to the training environment. Third, we investigate both the effect of MDZ administered at different times following reactivation on fear memory and the persistence of such effect 10 days after reactivation. Finally, we test whether the MDZ effect could be reverted by a single weak training trial (0.2 mA, 3 s) or by the presentation of the same unconditioned stimulus in the absence of the conditioned stimulus as a reminder which proves to induce significant freezing in rats not previously trained. Results show that MDZ interferes with the formation of a contextual fear memory only when administered after the reactivation procedure but not after the training procedure. This interference was effective up to 60 min after reactivation and not at a later time. No spontaneous recovery of freezing behavior was observed 11 days after MDZ injection which was not reverted by a weak training trial and by the unconditioned stimulus alone. All these data support the idea that stimulating GABA A receptor sites via MDZ selectively disrupts the reconsolidation process of a contextual fear memory. © 2006 IBRO. |
| format |
JOUR |
| author |
Bustos, S.G. Maldonado, H. Molina, V.A. |
| author_facet |
Bustos, S.G. Maldonado, H. Molina, V.A. |
| author_sort |
Bustos, S.G. |
| title |
Midazolam disrupts fear memory reconsolidation |
| title_short |
Midazolam disrupts fear memory reconsolidation |
| title_full |
Midazolam disrupts fear memory reconsolidation |
| title_fullStr |
Midazolam disrupts fear memory reconsolidation |
| title_full_unstemmed |
Midazolam disrupts fear memory reconsolidation |
| title_sort |
midazolam disrupts fear memory reconsolidation |
| url |
http://hdl.handle.net/20.500.12110/paper_03064522_v139_n3_p831_Bustos |
| work_keys_str_mv |
AT bustossg midazolamdisruptsfearmemoryreconsolidation AT maldonadoh midazolamdisruptsfearmemoryreconsolidation AT molinava midazolamdisruptsfearmemoryreconsolidation |
| _version_ |
1807322465621245952 |