Uroporhyrinogen decarboxylase from mouse mammary carcinoma and liver of normal and tumor-bearing mouse

1. 1. URO-D was investigated in crude extracts from mouse mammary carcinoma, normal mouse (NM) liver and tumor-bearing mouse (TBM) liver. 2. 2. URO-D from TBM liver and tumor appears to be more sensitive to increasing concentrations of UROgen than the NM liver enzyme. 3. 3. In tumor the rate-limitin...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Navone, N.M., Afonso, S.G., Polo, C.F., del C. Battle, A.M.
Formato: JOUR
Materias:
uroporphyrinogen decarboxylase
animal tissue
article
breast cancer
liver
mouse
nonhuman
ph
priority journal
Animal
Hydrogen-Ion Concentration
Kinetics
Liver
Male
Mammary Neoplasms, Experimental
Mice
Mice, Inbred BALB C
Substrate Specificity
Support, Non-U.S. Gov't
Temperature
Uroporphyrinogen Decarboxylase
Uroporphyrinogens
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03050491_v102_n1_p87_Navone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. URO-D was investigated in crude extracts from mouse mammary carcinoma, normal mouse (NM) liver and tumor-bearing mouse (TBM) liver. 2. 2. URO-D from TBM liver and tumor appears to be more sensitive to increasing concentrations of UROgen than the NM liver enzyme. 3. 3. In tumor the rate-limiting step seems to be the decarboxylation of the first carboxyl group, but this was not so clear for the NM and the TBM liver URO-D. 4. 4. URO-D activity was enhanced when incubated at higher temperatures in the presence of its substrate, suggesting that UROgen might afford some protection of the enzyme against heat inactivation. 5. 5. The optimum pH for all three sources is around 7.0. © 1992.

Ejemplares similares