Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex
The protein substrates affected by staurosporine (SP), the most potent inhibitor of protein kinases yet described, are unknown. In order to approach this problem we incubated cerebral cortex tissue with 0, 20, 50 and 100 nM of SP using [32P]orthophosphate as radioactive precursor. The analysis of th...
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todo:paper_03043940_v180_n1_p17_SebastianYakisich2023-10-03T15:20:17Z Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex Sebastian Yakisich, J. Radrizzani, M. Vargas, V.I. Cerebral cortex Development Phosphoprotein Phosphorylation Protein kinase Staurosporine staurosporine adolescent animal tissue article brain cortex brain development controlled study gel electrophoresis newborn nonhuman priority journal protein phosphorylation rat Alkaloids Animal Autoradiography Cerebral Cortex Electrophoresis, Polyacrylamide Gel Indicators and Reagents Nerve Tissue Proteins Phosphorylation Protein Kinase C Rats Rats, Wistar Rosaniline Dyes Staurosporine The protein substrates affected by staurosporine (SP), the most potent inhibitor of protein kinases yet described, are unknown. In order to approach this problem we incubated cerebral cortex tissue with 0, 20, 50 and 100 nM of SP using [32P]orthophosphate as radioactive precursor. The analysis of the phosphoproteins were made with a modified high resolution two dimensional gel electrophoresis, followed by autoradiography. We detected several proteins affected by SP. Specially noticeable was an approximately 55 kDa protein which strikingly diminished the intensity of phosphorylation. However, the reverse phenomenon was also observed. To the best of our knowledge this is the first examination of protein substrates affected by SP in intact tissue. © 1994. Fil:Radrizzani, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03043940_v180_n1_p17_SebastianYakisich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cerebral cortex Development Phosphoprotein Phosphorylation Protein kinase Staurosporine staurosporine adolescent animal tissue article brain cortex brain development controlled study gel electrophoresis newborn nonhuman priority journal protein phosphorylation rat Alkaloids Animal Autoradiography Cerebral Cortex Electrophoresis, Polyacrylamide Gel Indicators and Reagents Nerve Tissue Proteins Phosphorylation Protein Kinase C Rats Rats, Wistar Rosaniline Dyes Staurosporine |
| spellingShingle |
Cerebral cortex Development Phosphoprotein Phosphorylation Protein kinase Staurosporine staurosporine adolescent animal tissue article brain cortex brain development controlled study gel electrophoresis newborn nonhuman priority journal protein phosphorylation rat Alkaloids Animal Autoradiography Cerebral Cortex Electrophoresis, Polyacrylamide Gel Indicators and Reagents Nerve Tissue Proteins Phosphorylation Protein Kinase C Rats Rats, Wistar Rosaniline Dyes Staurosporine Sebastian Yakisich, J. Radrizzani, M. Vargas, V.I. Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| topic_facet |
Cerebral cortex Development Phosphoprotein Phosphorylation Protein kinase Staurosporine staurosporine adolescent animal tissue article brain cortex brain development controlled study gel electrophoresis newborn nonhuman priority journal protein phosphorylation rat Alkaloids Animal Autoradiography Cerebral Cortex Electrophoresis, Polyacrylamide Gel Indicators and Reagents Nerve Tissue Proteins Phosphorylation Protein Kinase C Rats Rats, Wistar Rosaniline Dyes Staurosporine |
| description |
The protein substrates affected by staurosporine (SP), the most potent inhibitor of protein kinases yet described, are unknown. In order to approach this problem we incubated cerebral cortex tissue with 0, 20, 50 and 100 nM of SP using [32P]orthophosphate as radioactive precursor. The analysis of the phosphoproteins were made with a modified high resolution two dimensional gel electrophoresis, followed by autoradiography. We detected several proteins affected by SP. Specially noticeable was an approximately 55 kDa protein which strikingly diminished the intensity of phosphorylation. However, the reverse phenomenon was also observed. To the best of our knowledge this is the first examination of protein substrates affected by SP in intact tissue. © 1994. |
| format |
JOUR |
| author |
Sebastian Yakisich, J. Radrizzani, M. Vargas, V.I. |
| author_facet |
Sebastian Yakisich, J. Radrizzani, M. Vargas, V.I. |
| author_sort |
Sebastian Yakisich, J. |
| title |
Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| title_short |
Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| title_full |
Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| title_fullStr |
Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| title_full_unstemmed |
Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| title_sort |
examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex |
| url |
http://hdl.handle.net/20.500.12110/paper_03043940_v180_n1_p17_SebastianYakisich |
| work_keys_str_mv |
AT sebastianyakisichj examinationofthenaturalproteinsubstratesaffectedbystaurosporineinthedevelopingcerebralcortex AT radrizzanim examinationofthenaturalproteinsubstratesaffectedbystaurosporineinthedevelopingcerebralcortex AT vargasvi examinationofthenaturalproteinsubstratesaffectedbystaurosporineinthedevelopingcerebralcortex |
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1807316421421563904 |