Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa

DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-f...

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Detalles Bibliográficos
Autores principales: Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T.
Formato: JOUR
Materias:
autophosphorylation
Ca2+
calmodulin
kinase
Neurospora crassa
protein kinase (calcium,calmodulin)
Ca(2+) Calmodulin Dependent Protein Kinase
calcium
cyclic AMP
egtazic acid
fungal protein
protein kinase
article
neurospora crassa
nonhuman
priority journal
enzymology
gel chromatography
ion exchange chromatography
isolation and purification
phosphorylation
polyacrylamide gel electrophoresis
protein processing
Ascomycota
Neurospora
Ca(2+)-Calmodulin Dependent Protein Kinase
Calcium
Calmodulin
Chromatography, DEAE-Cellulose
Chromatography, Gel
Cyclic AMP
Egtazic Acid
Electrophoresis, Polyacrylamide Gel
Fungal Proteins
Phosphorylation
Protein Kinases
Protein Processing, Post-Translational
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v102_n2_p155_Ulloa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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