Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein
Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubat...
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| Autores principales: | , , , , , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02681161_v10_n7_p1751_Brandelli |
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| Sumario: | Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (%ARt-%ARc): (%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM ≥ 0.2 was higher than in non-responders (STIM < 0.2); 72 ± 7% compared with 5 ± 3%. The overall predictive value of this test for adequate fertilization rate (>30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (>60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing ability in vitro. © 1995 Oxford University Press. |
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